Alignments for a candidate for gamP in Caulobacter crescentus NA1000

GapMind for catabolism of small carbon sources

Alignments for a candidate for gamP in Caulobacter crescentus NA1000

Align Putative PTS system glucosamine-specific EIICBA component; EC 2.7.1.193 (characterized)
to candidate CCNA_00458 CCNA_00458 PTS system, N-acetylglucosamine-specific IIBC component

Query= SwissProt::P39816
         (631 letters)



>FitnessBrowser__Caulo:CCNA_00458
          Length = 584

 Score =  364 bits (935), Expect = e-105
 Identities = 215/517 (41%), Positives = 310/517 (59%), Gaps = 47/517 (9%)

Query: 3   KKAFQILQQLGRALMTPVAVLPAAGLLLRFGDKDLLNIPIIKDA---------------G 47
           +   +ILQ LGRALM P+AVLP A LLLR G  DLL  P +  A               G
Sbjct: 2   RSPLEILQPLGRALMLPIAVLPVAALLLRIGQPDLLGAPALAAATHGMSLSVANTFGAAG 61

Query: 48  GVVFDNLPLIFAVGVAIGLAG-GEGVAGLAAVIGYLILTVTLDNMGKLLGLQPP------ 100
           G +F +L LIFA+GVA+GLA    G AGLA V+ Y+I T  ++     L + PP      
Sbjct: 62  GAIFASLGLIFAIGVAVGLARENHGAAGLAGVVCYVIATKGVE----ALLVAPPEVAAKA 117

Query: 101 YEGAEHLI----------DMGVFGGIIIGLLAAYLYKRFSSIELHPVLGFFSGKRFVPII 150
            EGA+ L            + +  GI+ G+++  LY R+S+I+L   L FF G+RFVPI+
Sbjct: 118 VEGAKDLAIAAWKAKEIGKLSIPVGILSGVISGALYNRYSTIQLPEYLAFFGGRRFVPIV 177

Query: 151 TSVSSLVIGVIFSFVWPLIQNGINAASSLI-ADSTVGLFFYATIYRLLIPFGLHHIFYTP 209
             ++ +V+ ++F   W  ++ G++  S L+ A   +GL  Y  + RLLI  GLHHI    
Sbjct: 178 AGLAGVVLALLFGAFWSTLEAGVDGLSGLVTASGDLGLVVYGLLNRLLIVTGLHHILNNV 237

Query: 210 FYFMMGEYTDPSTGNTVTGDLTRFFAGDPTAGRFMMGDFPYMIFCLPAVALAIIHTARPE 269
            +F++G++      N VTGDL RF AGD TAG FM G FP M+F LPA  LA++HTARPE
Sbjct: 238 VWFILGDF------NGVTGDLNRFAAGDKTAGAFMSGFFPVMMFGLPAACLAMLHTARPE 291

Query: 270 KKKMISGVMISAALTSMLTGITEPVEFSFLFVAPVLYLINSILAGVIFVVCDLFHVRHGY 329
           ++K ++G++ S ALTS LTG+TEP+EF+F+F+APVL+ I+++L G+   + ++  V+ G+
Sbjct: 292 RRKAVAGMLGSLALTSFLTGVTEPIEFTFMFLAPVLFAIHALLTGLSMALMNMLDVKLGF 351

Query: 330 TFSGGGIDYVLNYGLSTNGWVVIPVGIVFAFIYYYLFRFAILKWNLKTPGRETDEDGQNE 389
            FS G  DYVLN+  +T   ++IPVG+V+  +YY +FRFAIL+++LKTPGRE  ++    
Sbjct: 352 GFSAGLFDYVLNFNKATRPLLLIPVGLVYGALYYGVFRFAILRFDLKTPGRE--DEAPPS 409

Query: 390 EKAPVAKDQLAFHVLQALGGQQNIANLDACITRLRVTVHQPSQVCKDELKRLGAVGVLEV 449
            +A          +L ALGG  N+ ++DAC TRLR+ V     V +  LK LGA GV+  
Sbjct: 410 AQAVTTGGGRGADMLAALGGAANLVSVDACTTRLRLIVVDQGLVNEPALKALGARGVVRP 469

Query: 450 NNN-FQAIFGTKSDALKDDIK-TIMAGGVPATAAALD 484
           ++   Q + G  +D +  +I+  I A   PA A A D
Sbjct: 470 SDKALQVVLGPIADTVAGEIRHAISAPPAPAPAPAPD 506



 Score = 34.3 bits (77), Expect = 2e-05
 Identities = 19/55 (34%), Positives = 29/55 (52%)

Query: 398 QLAFHVLQALGGQQNIANLDACITRLRVTVHQPSQVCKDELKRLGAVGVLEVNNN 452
           Q A  +L ALGG  N+ +L    +RLRV ++ P +V +  L   G  G + V  +
Sbjct: 509 QAAKALLAALGGADNLRDLSVHASRLRVVLNDPQRVDQAALHTAGVRGFVRVGEH 563


Lambda     K      H
   0.324    0.142    0.421 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 948
Number of extensions: 56
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 631
Length of database: 584
Length adjustment: 37
Effective length of query: 594
Effective length of database: 547
Effective search space:   324918
Effective search space used:   324918
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources