Align HutV aka HISV aka R02702 aka SMC00670, component of Uptake system for hisitidine, proline, proline-betaine and glycine-betaine (characterized)
to candidate CCNA_03810 CCNA_03810 organic solvent resistance transport system ATP-binding protein
Query= TCDB::Q9KKE1 (275 letters) >FitnessBrowser__Caulo:CCNA_03810 Length = 256 Score = 130 bits (328), Expect = 2e-35 Identities = 85/236 (36%), Positives = 127/236 (53%), Gaps = 6/236 (2%) Query: 43 LNDVSLKIGAGKIFVIMGLSGSGKSTLVRHINRLIEPTSGEVLFDGDNILDLGAKALRAF 102 L+ + L + G+ VI+G SG GKS ++ L+ P +G + DG N++ L R Sbjct: 23 LDQLDLSVAPGRSLVIIGGSGQGKSVTIKTALGLLRPEAGAIELDGKNVVGLSETQRRKL 82 Query: 103 RMRRVSMVFQSFALMPHRTVLQNVVYGQ-RVRGVSKDDAREIGMKWIDTVGLSGYDA-KF 160 RV ++FQ AL T+ +NV + GV + AR+ ++ ++ V LS A +F Sbjct: 83 -FSRVGVLFQGAALFDSLTIWENVAFRLINADGVPRRKARDRAIEALEQVRLSADVAERF 141 Query: 161 PHQLSGGMKQRVGLARALAADTDVILMDEAFSALDPLIRGDMQDQLLQLQRNLAKTIVFI 220 P +LSGGM++R GLARA+ A +++ DE + LDP+ + + R L T V I Sbjct: 142 PSELSGGMQKRAGLARAVVARPEILFFDEPTTGLDPITAAAINQLISDQVRRLGSTAVSI 201 Query: 221 THDLDEALRIGSEIAILRDGQVVQVGTPNDILDNPANDYVARFVQRRHERP--QTH 274 THDL A IG EIA+L G+++ G P L N YV +FV+ R E P +TH Sbjct: 202 THDLASAQTIGDEIAMLHGGKIIWRG-PAAELATTDNPYVRQFVEGRAEGPISETH 256 Lambda K H 0.323 0.139 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 174 Number of extensions: 11 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 275 Length of database: 256 Length adjustment: 25 Effective length of query: 250 Effective length of database: 231 Effective search space: 57750 Effective search space used: 57750 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 47 (22.7 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory