Align subunit of β-ketoadipyl CoA thiolase (EC 2.3.1.174; EC 2.3.1.16) (characterized)
to candidate CCNA_02490 CCNA_02490 3-ketoacyl-CoA thiolase
Query= metacyc::MONOMER-3207 (400 letters) >FitnessBrowser__Caulo:CCNA_02490 Length = 399 Score = 564 bits (1454), Expect = e-165 Identities = 286/400 (71%), Positives = 331/400 (82%), Gaps = 1/400 (0%) Query: 1 MRDVFICDAIRTPIGRFGGALAGVRADDLAAVPLKALIEPNPAVQWDQVDEVFFGCANQA 60 M +ICD IRTPIGR+GG+L+ VRADDLAA+PLKAL+ NP++ +DE+ G ANQA Sbjct: 1 MTAAYICDGIRTPIGRYGGSLSKVRADDLAAIPLKALVARNPSLDLAAIDEIVLGSANQA 60 Query: 61 GEDNRNVARMALLLAGLPESIPGVTLNRLCASGMDAIGTAFRAIASGEMELAIAGGVESM 120 GEDNRNVARMALLLAG P S+PGVT+NRLCASG++A+G A RAIASG +L IAGGVESM Sbjct: 61 GEDNRNVARMALLLAGYPVSVPGVTVNRLCASGLEAVGYAARAIASGHNDLVIAGGVESM 120 Query: 121 SRAPFVMGKAESGYSRNMKLEDTTIGWRFINPLMKSQYGVDSMPETADNVADDYQVSRAD 180 SRAPFVMGKA+S +SR+ ++ DTTIGWRF+NP M+ YGVDSMPETA+NVA DY V+R D Sbjct: 121 SRAPFVMGKADSAFSRSAEIFDTTIGWRFVNPAMRKLYGVDSMPETAENVATDYGVNRED 180 Query: 181 QDAFALRSQQKAAAAQAAGFFAEEIVPVRIAHKKGETIVERDEHLRPETTLEALTKLKPV 240 QDAFALRSQ + AAAQA GF A EI PV I K G TIV+RDEH R ETT+EAL KLKP+ Sbjct: 181 QDAFALRSQARTAAAQANGFLAGEITPVEIPGKAGPTIVDRDEHPR-ETTMEALAKLKPI 239 Query: 241 NGPDKTVTAGNASGVNDGAAALILASAEAVKKHGLTPRARVLGMASGGVAPRVMGIGPVP 300 TVTAGNASGVNDGA AL++AS +AVK+HGLTPRAR+ G AS GV PRVMGIGPVP Sbjct: 240 VREGGTVTAGNASGVNDGAVALVIASEDAVKRHGLTPRARITGYASAGVEPRVMGIGPVP 299 Query: 301 AVRKLTERLGVAVSDFDVIELNEAFASQGLAVLRELGVADDAPQVNPNGGAIALGHPLGM 360 AVRKL + G+A+ DFDV+ELNEAFA+QGLAVLR+LG+ DD VN NGGAIALGHPLG Sbjct: 300 AVRKLMAKTGLAIGDFDVVELNEAFAAQGLAVLRQLGLPDDGAHVNANGGAIALGHPLGA 359 Query: 361 SGARLVLTALHQLEKSGGRKGLATMCVGVGQGLALAIERV 400 SGARLVLTAL QLE SGG++GLAT+C+GVGQG ALA ERV Sbjct: 360 SGARLVLTALRQLEASGGQRGLATLCIGVGQGAALAFERV 399 Lambda K H 0.318 0.134 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 551 Number of extensions: 17 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 400 Length of database: 399 Length adjustment: 31 Effective length of query: 369 Effective length of database: 368 Effective search space: 135792 Effective search space used: 135792 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 50 (23.9 bits)
Align candidate CCNA_02490 CCNA_02490 (3-ketoacyl-CoA thiolase)
to HMM TIGR01930 (acetyl-CoA C-acyltransferase (EC 2.3.1.16))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR01930.hmm # target sequence database: /tmp/gapView.1778504.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01930 [M=385] Accession: TIGR01930 Description: AcCoA-C-Actrans: acetyl-CoA C-acyltransferase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 8e-139 448.8 2.8 9e-139 448.6 2.8 1.0 1 lcl|FitnessBrowser__Caulo:CCNA_02490 CCNA_02490 3-ketoacyl-CoA thiola Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__Caulo:CCNA_02490 CCNA_02490 3-ketoacyl-CoA thiolase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 448.6 2.8 9e-139 9e-139 1 385 [] 6 397 .. 6 397 .. 0.95 Alignments for each domain: == domain 1 score: 448.6 bits; conditional E-value: 9e-139 TIGR01930 1 ivdavRtpigklggslkelsaedLlaavikellera.gldpekidevilGnvlqageq.aniaReaalaaglp 71 i d++Rtpig++ggsl++++a+dL+a +k+l++r+ ld ++ide++lG + qage+ n+aR+a+l ag p lcl|FitnessBrowser__Caulo:CCNA_02490 6 ICDGIRTPIGRYGGSLSKVRADDLAAIPLKALVARNpSLDLAAIDEIVLGSANQAGEDnRNVARMALLLAGYP 78 789*******99**********************************************9************** PP TIGR01930 72 esvpaltvnrvCaSglqAvalaaqkikaGeadvvvaGGvEsmSrvpillkaslrreslklgkakledqllkdl 144 svp++tvnr+CaSgl+Av aa++i++G++d+v+aGGvEsmSr+p+++ ++ +s+ ++a+ d+++ + lcl|FitnessBrowser__Caulo:CCNA_02490 79 VSVPGVTVNRLCASGLEAVGYAARAIASGHNDLVIAGGVESMSRAPFVMGKA---DSAFSRSAEIFDTTI--G 146 ***********************************************99997...444445555555333..1 PP TIGR01930 145 ..........vktklsmgetAenlakkygisReeqDeyalrShqkaakAieegkfkdeivpvevkgk..kkvv 205 + sm etAen+a++yg++Re+qD++alrS+ ++a+A+++g+++ ei+pve++gk +++v lcl|FitnessBrowser__Caulo:CCNA_02490 147 wrfvnpamrkLYGVDSMPETAENVATDYGVNREDQDAFALRSQARTAAAQANGFLAGEITPVEIPGKagPTIV 219 3467888888667789********************************************************* PP TIGR01930 206 skDegirpnttlekLakLkpafkekkgstvtAgNssqlnDGAaalllmseevakelgltplarivsaavagvd 278 ++De++r +tt+e+LakLkp+ +e g tvtAgN+s++nDGA+al+++se+++k++gltp ari ++a agv+ lcl|FitnessBrowser__Caulo:CCNA_02490 220 DRDEHPR-ETTMEALAKLKPIVRE-GG-TVTAGNASGVNDGAVALVIASEDAVKRHGLTPRARITGYASAGVE 289 *******.89************96.68.7******************************************** PP TIGR01930 279 peemglgpvpAiekaLkkaglsisdidlvEinEAFAaqvlavekelgsld.lekvNvnGGAiAlGHPlGasGa 350 p++mg+gpvpA++k+++k+gl i d+d+vE+nEAFAaq lav ++lg+ d + vN nGGAiAlGHPlGasGa lcl|FitnessBrowser__Caulo:CCNA_02490 290 PRVMGIGPVPAVRKLMAKTGLAIGDFDVVELNEAFAAQGLAVLRQLGLPDdGAHVNANGGAIALGHPLGASGA 362 ***********************************************854699******************** PP TIGR01930 351 rivltllkeLkergkkyGlatlCvggGqGaAvile 385 r+vlt l++L+ g+++GlatlC+g GqGaA+ +e lcl|FitnessBrowser__Caulo:CCNA_02490 363 RLVLTALRQLEASGGQRGLATLCIGVGQGAALAFE 397 *******************************9886 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (385 nodes) Target sequences: 1 (399 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.00 # Mc/sec: 23.62 // [ok]
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory