GapMind for catabolism of small carbon sources

 

Aligments for a candidate for iolA in Caulobacter crescentus NA1000

Align malonate-semialdehyde dehydrogenase (acetylating) (EC 1.2.1.18) (characterized)
to candidate CCNA_03243 CCNA_03243 NADP+-dependent gamma-glutamyl-gamma-aminobutyraldehyde dehydrogenase

Query= reanno::Smeli:SMc00781
         (498 letters)



>lcl|FitnessBrowser__Caulo:CCNA_03243 CCNA_03243 NADP+-dependent
           gamma-glutamyl-gamma-aminobutyraldehyde dehydrogenase
          Length = 499

 Score =  207 bits (526), Expect = 9e-58
 Identities = 146/466 (31%), Positives = 231/466 (49%), Gaps = 27/466 (5%)

Query: 8   IDGKRVAGTSGRVSNIFNPATGEVQGTVALASDADLAAAVESAKAA--QPKWAATNPQRR 65
           IDG  V   SG   +  +P  G+V   V      D+  AV  A+AA    +W    P+++
Sbjct: 25  IDGDLVEAASGATFHNVSPRDGQVLNLVTACQADDVERAVAGARAAFEDGRWRDQGPRQK 84

Query: 66  ARVFMKFVQLLNDNMNELAEMLSREHGKTIDDAKG-DIVRGLEVCEFVIGIPHLQKSEFT 124
             V  +  +L+  + +ELA + S + GK I DA+  DI   +  C +      L K    
Sbjct: 85  KAVLFRLAELMERDADELALLESLDVGKPISDARNVDIPLAINTCRWYAEA--LDKVYGE 142

Query: 125 EGAGPGIDM-YSIRQPVGIGAGITPFNFPGMIPMWMFAPAIACGNAFILKPSERDPSVPI 183
            G  P   + Y++ +P+G+   I P+NFP  + MW  APA+A GN+ +LKP+E+ P   +
Sbjct: 143 VGTSPADRLSYAVHEPLGVIGAIVPWNFPLHMAMWKVAPALAMGNSVVLKPAEQSPLTAL 202

Query: 184 RLAELMIEAGLPAGILNVVNGDKG-AVDAILTHPDIAAVSFVGSTPIARYVYG-TAAMNG 241
           +L  L +EAGLP G+LNV+ G  G A +A+    D+  ++F GS P+ R +   +A  N 
Sbjct: 203 KLGALALEAGLPPGVLNVIPGLGGVAGEALALSMDVDMIAFTGSGPVGRRLMEYSARSNL 262

Query: 242 KRAQCFGGAKNHMIIMPDA-DLDQAANALIGAGYGSAGERCMAIS---VAVPVGEETANR 297
           KR     G K+  I+  D  DL+ AA A     + + GE C A S   V  P+ +    R
Sbjct: 263 KRVSLELGGKSPQIVFADCPDLEAAAQAAAWGVFYNQGEVCTAASRLLVEAPIKDAFLAR 322

Query: 298 LIDKLVPMVESLRIGPYTDEKADMGPVVTKEAEQRIRSLIDSGIEQGAKLVVDGRDFKLQ 357
           +I+    + + +++G   D     G +V++         I +   QGA+ V+ G+  +++
Sbjct: 323 VIE----VAKGMKVGDPLDPSTQFGAMVSERQMNTALDYIATADSQGARRVLGGQ--RVR 376

Query: 358 GYENGHFIGGCLFDDVTPDMDIYKTEIFGPVLSVVRARNYEEALSLPMKHEYGNGVAIYT 417
               G ++   +FD V PD  + + E+FGPVL V+   + +EA+ L     YG    ++T
Sbjct: 377 QEAGGFYVEPTIFDQVRPDQTLAREEVFGPVLGVMTFSSEDEAMRLANDTVYGLAAGLWT 436

Query: 418 RDGDAARDFASRINIGMVGVN----VPIPVPLAYHSFGGWKSSSFG 459
            D   A   A R+  G+V VN      I +P     FGG+K S FG
Sbjct: 437 ADVSKALRGARRLKAGLVWVNGWDACDITMP-----FGGFKQSGFG 477


Lambda     K      H
   0.319    0.137    0.409 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 523
Number of extensions: 25
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 498
Length of database: 499
Length adjustment: 34
Effective length of query: 464
Effective length of database: 465
Effective search space:   215760
Effective search space used:   215760
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory