Aligments for a candidate for liuD in Caulobacter crescentus NA1000

GapMind for catabolism of small carbon sources

Aligments for a candidate for liuD in Caulobacter crescentus NA1000

Align Methylcrotonoyl-CoA carboxylase (EC 6.4.1.4) (characterized)
to candidate CCNA_02252 CCNA_02252 methylcrotonyl-CoA carboxylase carboxyl transferase subunit

Query= reanno::Smeli:SM_b21122
         (535 letters)



>lcl|FitnessBrowser__Caulo:CCNA_02252 CCNA_02252 methylcrotonyl-CoA
           carboxylase carboxyl transferase subunit
          Length = 530

 Score =  737 bits (1903), Expect = 0.0
 Identities = 361/535 (67%), Positives = 431/535 (80%), Gaps = 5/535 (0%)

Query: 1   MTVLRSHISPSSEEFKANRAAMTEAIATIEDAVRLAAAGGGETARERHVSRGKLLPRDRL 60
           M  L S I  SS+ F  N A     +  +   V  AA GG E+ARERH +RGKLLPR+R+
Sbjct: 1   MPKLNSVIDASSDAFAKNAAHNRALVDELRAKVAQAALGGPESARERHTARGKLLPRERV 60

Query: 61  ATLIDPGTPFLEVGATAAYGMYNDDAPGAGLITGIGRISARECMIVCNDPTVKGGTYYPL 120
             L+DPG+PFLEVG  AA  +YN +APGAG+I G+GR+S RE MIV NDPTVKGG Y+P+
Sbjct: 61  ERLLDPGSPFLEVGQLAACDLYNGEAPGAGMICGVGRVSGREVMIVANDPTVKGGAYFPM 120

Query: 121 TVKKHLRAQEIAAENRLPCVYLVDSGGANLPNQDEVFPDRDHFGRIFYNQANMSAAGIPQ 180
           TVKKHLRAQEIAA+NRLPC+YLVDSGGANLP+Q EVFPDRDHFGRIF+NQA MSAA IPQ
Sbjct: 121 TVKKHLRAQEIAAQNRLPCLYLVDSGGANLPHQAEVFPDRDHFGRIFFNQARMSAASIPQ 180

Query: 181 IAVVMGSCTAGGAYVPAMSDEAIIVEKQGTIFLAGPPLVRAATGEVVSAEDLGGADVHTR 240
           IA VMGSCTAGGAYVPAMSDE +IV  QGTIFLAGPPLV+AATGEV+SAE+LGGA+ H R
Sbjct: 181 IACVMGSCTAGGAYVPAMSDETVIVRNQGTIFLAGPPLVKAATGEVISAEELGGAETHGR 240

Query: 241 LSGVADHLARDDAHALALARRAVSALNREKPWTVERIEPEPPLYDPEEIAGIVPADLKTP 300
            SGV DH+A +D HAL + R  V+ LN  KP  +   +PEPP YDPEE+ GIVP D++ P
Sbjct: 241 RSGVVDHVAENDEHALEIVRSIVANLNTTKPDQLVLADPEPPAYDPEELYGIVPTDVRAP 300

Query: 301 YEIREVIARLVDGSRFDEFKARFGTTLVCGFAHVHGIPVGIVANNGVLFSESAVKGAHFV 360
           Y++REVIAR+VDGS+FDEFKA +GTTLVCGFA + G PV I+ANNGVLFSESA+KGAHF+
Sbjct: 301 YDVREVIARIVDGSQFDEFKALYGTTLVCGFARIWGQPVAILANNGVLFSESALKGAHFI 360

Query: 361 ELCAQRRIPLVFLQNITGFMVGRKYETEGIAKHGAKLVTAVATVKVPKITMLVGGSFGAG 420
           EL  +R+IPL+FLQNI+GFMVG KYE  GIAK GAKLVTAVA+ +VPK T+L+GGSFGAG
Sbjct: 361 ELACKRKIPLIFLQNISGFMVGGKYEAGGIAKDGAKLVTAVASAEVPKFTVLIGGSFGAG 420

Query: 421 NYGMCGRAFSPRFLWTWPNSRISVMGGEQAAGVLSSVRGEALKRSGKPWSEEEEARFRQP 480
           NYGMCGRA+SPRFL+TWPNSRISVMGGEQAA VL++V  +A K     WS EE   F+ P
Sbjct: 421 NYGMCGRAYSPRFLFTWPNSRISVMGGEQAASVLATVHRDAAK-----WSPEEAEAFKAP 475

Query: 481 VLDLFERQSHPLYASARLWDDGVIDPRKSRDVLALSLSAALNAPIEETRFGLFRM 535
           +   +E + +P +A+ARLWDDG+IDP ++RD L L++SA+LNAPI ET FG+FRM
Sbjct: 476 IRQRYEDEGNPYHATARLWDDGIIDPAQTRDALGLAISASLNAPIPETTFGVFRM 530


Lambda     K      H
   0.320    0.136    0.404 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 893
Number of extensions: 34
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 535
Length of database: 530
Length adjustment: 35
Effective length of query: 500
Effective length of database: 495
Effective search space:   247500
Effective search space used:   247500
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources