GapMind for catabolism of small carbon sources

 

Aligments for a candidate for livG in Caulobacter crescentus NA1000

Align ABC transporter ATP-binding protein (characterized, see rationale)
to candidate CCNA_00903 CCNA_00903 inositol transport ATP-binding protein IatA

Query= uniprot:A0A165KC86
         (260 letters)



>lcl|FitnessBrowser__Caulo:CCNA_00903 CCNA_00903 inositol transport
           ATP-binding protein IatA
          Length = 515

 Score =  126 bits (316), Expect = 1e-33
 Identities = 76/243 (31%), Positives = 134/243 (55%), Gaps = 13/243 (5%)

Query: 9   VLKVAGISKRFGGLQALSDVGITIKRGQVYGLIGPNGAGKTTFFNVITGLYTPDAGTFEL 68
           +L V+ +SK F G++AL  V + +  G+V+ L+G NGAGK+T   +++  +  DAGT   
Sbjct: 3   LLDVSQVSKSFPGVRALDQVDLVVGVGEVHALLGENGAGKSTLIKILSAAHAADAGTVTF 62

Query: 69  AGKPYEPT-AVHEVAKAGIARTFQNIRLFAEMTALENVMVGRHIRTGSGLFGAVFRTKGF 127
           AG+  +P  A     + GIA  +Q   LF E++  EN+ +GR  R      G V      
Sbjct: 63  AGQVLDPRDAPLRRQQLGIATIYQEFNLFPELSVAENMYLGREPRR----LGLV------ 112

Query: 128 KAEEAAIAKRAQELLDYVGIGKFADYKARTLSYGDQRRLEIARALATDPQLIALDEPAAG 187
             + + +   AQ LL+ +G+    D   R L+  +Q+ +EIA+A+  + +LI +DEP A 
Sbjct: 113 --DWSRLRADAQALLNDLGLPLNPDAPVRGLTVAEQQMVEIAKAMTLNARLIIMDEPTAA 170

Query: 188 MNATEKVQLRELIDRIRNDNRTILLIEHDVKLVMGLCDRVTVLDYGKQIAEGNPAEVQKN 247
           ++  E  +L  +I  ++  + +++ + H +  V  +CDR TV+  G+ +A G+ A+V+  
Sbjct: 171 LSGREVDRLHAIIAGLKARSVSVIYVSHRLGEVKAMCDRYTVMRDGRFVASGDVADVEVA 230

Query: 248 EKV 250
           + V
Sbjct: 231 DMV 233



 Score = 69.7 bits (169), Expect = 1e-16
 Identities = 65/259 (25%), Positives = 117/259 (45%), Gaps = 20/259 (7%)

Query: 8   VVLKVAGISKRFGGLQA---LSDVGITIKRGQVYGLIGPNGAGKTTFFNVITGLYTPDAG 64
           VVLKV G++     L A   L  V    + G++ GL G  GAG+T    +I G     AG
Sbjct: 255 VVLKVEGVTPAAPRLSAPGYLRQVSFAARGGEIVGLAGLVGAGRTDLARLIFGADPIAAG 314

Query: 65  TFELAGKPYEPTAVHEVAKAGIARTFQNIRLFAEMTALENVMVGRHIRTGSGL-----FG 119
              +  KP    +  +  +AGI        L  E    +   +   IR    L       
Sbjct: 315 RVLVDDKPLRLRSPRDAIQAGIM-------LVPEDRKQQGCFLDHSIRRNLSLPSLKALS 367

Query: 120 AVFRTKGFKAEEAAIAKRAQELLDYVGIGKFADYKARTLSYGDQRRLEIARALATDPQLI 179
           A+ +    +AE   +    Q+L   +   + A  K   LS G+Q+++ + RA+A  P+++
Sbjct: 368 ALGQWVDERAERDLVETYRQKLRIKMADAETAIGK---LSGGNQQKVLLGRAMALTPKVL 424

Query: 180 ALDEPAAGMNATEKVQLRELIDRIRNDNRTILLIEHDVKLVMGLCDRVTVLDYGKQIAEG 239
            +DEP  G++   K ++ +++  + +    +++I  ++  VM + DR+ V   G  +A+ 
Sbjct: 425 IVDEPTRGIDIGAKAEVHQVLSDLADLGVAVVVISSELAEVMAVSDRIVVFREGVIVADL 484

Query: 240 NPAEVQKNEKVIEAYLGTG 258
           +       E+ + AY+ TG
Sbjct: 485 DAQTA--TEEGLMAYMATG 501


Lambda     K      H
   0.319    0.137    0.384 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 304
Number of extensions: 21
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 260
Length of database: 515
Length adjustment: 29
Effective length of query: 231
Effective length of database: 486
Effective search space:   112266
Effective search space used:   112266
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 49 (23.5 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory