GapMind for catabolism of small carbon sources

 

Aligments for a candidate for lysN in Caulobacter crescentus NA1000

Align 2-aminoadipate transaminase (EC 2.6.1.39) (characterized)
to candidate CCNA_00620 CCNA_00620 acetylornithine aminotransferase/succinyldiaminopimelate aminotransferase

Query= reanno::Putida:PP_4108
         (416 letters)



>lcl|FitnessBrowser__Caulo:CCNA_00620 CCNA_00620 acetylornithine
           aminotransferase/succinyldiaminopimelate
           aminotransferase
          Length = 392

 Score =  179 bits (453), Expect = 2e-49
 Identities = 137/410 (33%), Positives = 198/410 (48%), Gaps = 44/410 (10%)

Query: 16  ITLSHGRNAEVWDTDGKRYIDFVGGIGVLNLGHCNPAVVEAIQAQATRLTHYAFNAAPHG 75
           I +  G    ++D DG+ Y+D   G+ V  LGH +P +V+A++ QA  L H A N     
Sbjct: 13  IDIVRGEGCWLYDQDGRDYLDLAAGVAVNTLGHGDPRLVQALKTQADILWH-ASNLYRLP 71

Query: 76  PYLALMEQLSQFVPVSYPLAGMLTNSGAEAAENALKVAR---GATGKRA---IIAFDGGF 129
              AL  +L+     ++       NSGAEA E A+K AR   GA G+     ++ F   F
Sbjct: 72  AQEALATKLTD---ATFADRVFFANSGAEAVEAAIKTARRWQGAKGRPERYRVLTFGNAF 128

Query: 130 HGRTLATLNLNGKVAPYKQRVGELPGPVYHLPYPSADTGV--TCEQALKAMDRLFSVELA 187
           HGRTLAT++   ++   K R G  P       Y + DT      E A +A+         
Sbjct: 129 HGRTLATISATDQM---KVREGFTP------LYDAFDTTPFNDIEGAARAI--------- 170

Query: 188 VEDVAAFIFEPVQGEGGFLALDPAFAQALRRFCDERGILIIIDEIQSGFGRTGQRFAFPR 247
               AA + EP+QGEGG     P F   LR  CD+  +L+I+DE+Q+G GRTG  FA   
Sbjct: 171 TPQTAAILVEPIQGEGGLTPATPGFLAGLRALCDQHDLLLILDEVQTGIGRTGHLFAHEL 230

Query: 248 LGIEPDLLLLAKSIAGGMPLGAVVGRKELMAALPKGGLGGTYSGNPISCAAALASL-AQM 306
            G+ PD++ +AK + GG P+GA +  ++  + +  G  G TY GNP++CA A A L A +
Sbjct: 231 YGVRPDIIAVAKGLGGGFPIGACLATEDAASGMTPGSHGSTYGGNPLACAVASAVLDAVL 290

Query: 307 TDENLATWGERQEQAIVSRYERWKASGLSPYIGRLTGVGAMRGIEFANADGSPAPAQLAK 366
               L T   R+  A+V           S    R  G G MRG++          A    
Sbjct: 291 APGFLET--VRERAALVDALLERLLRRHSDLFVRAQGHGLMRGLQ--------VRASARD 340

Query: 367 VMEAARARGLLLMPSGKARHIIRLLAPLTI-EAEVLEEGLDILEQCLAEL 415
           V+   R  G++ + +G    ++RLL PLTI E E+ E    +L    A L
Sbjct: 341 VVAHLRDFGVMTVAAG--ADVVRLLPPLTISELEIAEAEARLLRAAEAWL 388


Lambda     K      H
   0.320    0.137    0.402 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 379
Number of extensions: 14
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 416
Length of database: 392
Length adjustment: 31
Effective length of query: 385
Effective length of database: 361
Effective search space:   138985
Effective search space used:   138985
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory