Align ABC-type maltose transporter (EC 7.5.2.1) (characterized)
to candidate CCNA_03235 CCNA_03235 spermidine/putrescine transport ATP-binding protein potA
Query= BRENDA::Q70HW1 (384 letters) >FitnessBrowser__Caulo:CCNA_03235 Length = 381 Score = 229 bits (585), Expect = 7e-65 Identities = 127/273 (46%), Positives = 171/273 (62%), Gaps = 5/273 (1%) Query: 21 VKDFNLDIQDKEFTVFVGPSGCGKTTTLRMIAGLEDITEGNLYIGDRRVNDVPPKDRDIA 80 V + +L + + EF +GPSGCGKTT LRM+AG E TEG + I + +++VPP R + Sbjct: 30 VDNVSLTVNEGEFFALLGPSGCGKTTLLRMLAGFETPTEGRILIDGQDISNVPPNKRPVN 89 Query: 81 MVFQNYALYPHMTVYQNMAFGLKLRKVPKAEIDRRVQEAAKILDIAHLLDRKPKALSGGQ 140 MVFQ+YA++PHMTV N+A+GLK+ VPKAE + RV EA +++ + L RKP LSGGQ Sbjct: 90 MVFQSYAVFPHMTVADNVAYGLKVDNVPKAEREARVAEALELVQLGGLGGRKPDQLSGGQ 149 Query: 141 RQRVALGRAIVREPQVFLMDEPLSNLDAKLRVQMRAEIRKLHQRLQTTVIYVTHDQTEAM 200 RQRVAL RA+V+ P+V L+DEPLS LDAKLR QMR E+ L +++ T I VTHDQ EA+ Sbjct: 150 RQRVALARALVKRPRVLLLDEPLSALDAKLREQMRTELCTLQEKVGITFIMVTHDQDEAL 209 Query: 201 TMGDRIVVMRDGVIQQADTPQVVYSQPKNMFVAGFIGSPAMNFIRGEIVQDGDAFYFRAP 260 + R VM G++QQ TP +Y P + FVA FIG +N G + D + A Sbjct: 210 ALASRCAVMSKGLLQQVATPSDLYEFPNSRFVADFIGQ--VNLFEGVLAVDEPS---HAV 264 Query: 261 SISLRLPEGRYGVLKASGAIGKPVVLGVRPEDL 293 S LP + +G G V +RPE + Sbjct: 265 IKSPDLPVDIFLDHGVTGPRGGTVWAAIRPEKI 297 Lambda K H 0.321 0.138 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 374 Number of extensions: 16 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 384 Length of database: 381 Length adjustment: 30 Effective length of query: 354 Effective length of database: 351 Effective search space: 124254 Effective search space used: 124254 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 50 (23.9 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory