Alignments for a candidate for frcA in Caulobacter crescentus NA1000

GapMind for catabolism of small carbon sources

Alignments for a candidate for frcA in Caulobacter crescentus NA1000

Align Fructose import ATP-binding protein FrcA; EC 7.5.2.- (characterized)
to candidate CCNA_00903 CCNA_00903 inositol transport ATP-binding protein IatA

Query= SwissProt::Q9F9B0
         (260 letters)



>FitnessBrowser__Caulo:CCNA_00903
          Length = 515

 Score =  142 bits (357), Expect = 2e-38
 Identities = 85/245 (34%), Positives = 141/245 (57%), Gaps = 10/245 (4%)

Query: 6   ILTARGLVKRYGRVTALDRADFDLYPGEILAVIGDNGAGKSSMIKAISGAVTPDEGEIRL 65
           +L    + K +  V ALD+ D  +  GE+ A++G+NGAGKS++IK +S A   D G +  
Sbjct: 3   LLDVSQVSKSFPGVRALDQVDLVVGVGEVHALLGENGAGKSTLIKILSAAHAADAGTVTF 62

Query: 66  EGKPIQFR-SPMEARQAGIETVYQNLALSPALSIADNMFLGREIRKPGIMGKWFRSLDRA 124
            G+ +  R +P+  +Q GI T+YQ   L P LS+A+NM+LGRE R+ G++       D +
Sbjct: 63  AGQVLDPRDAPLRRQQLGIATIYQEFNLFPELSVAENMYLGREPRRLGLV-------DWS 115

Query: 125 AMEKQARAKLSELGLMTIQNINQAVETLSGGQRQGVAVARAAAFGSKVVIMDEPTAALGV 184
            +   A+A L++LGL    N +  V  L+  ++Q V +A+A    ++++IMDEPTAAL  
Sbjct: 116 RLRADAQALLNDLGLPL--NPDAPVRGLTVAEQQMVEIAKAMTLNARLIIMDEPTAALSG 173

Query: 185 KESRRVLELILDVRRRGLPIVLISHNMPHVFEVADRIHIHRLGRRLCVINPKDYTMSDAV 244
           +E  R+  +I  ++ R + ++ +SH +  V  + DR  + R GR +   +  D  ++D V
Sbjct: 174 REVDRLHAIIAGLKARSVSVIYVSHRLGEVKAMCDRYTVMRDGRFVASGDVADVEVADMV 233

Query: 245 AFMTG 249
             M G
Sbjct: 234 RLMVG 238



 Score = 93.6 bits (231), Expect = 8e-24
 Identities = 70/248 (28%), Positives = 122/248 (49%), Gaps = 11/248 (4%)

Query: 6   ILTARGLVKRYGRVTA---LDRADFDLYPGEILAVIGDNGAGKSSMIKAISGAVTPDEGE 62
           +L   G+     R++A   L +  F    GEI+ + G  GAG++ + + I GA     G 
Sbjct: 256 VLKVEGVTPAAPRLSAPGYLRQVSFAARGGEIVGLAGLVGAGRTDLARLIFGADPIAAGR 315

Query: 63  IRLEGKPIQFRSPMEARQAGIETVYQNLALSPAL---SIADNMFLGREIRKPGIMGKWFR 119
           + ++ KP++ RSP +A QAGI  V ++          SI  N+ L   ++    +G+W  
Sbjct: 316 VLVDDKPLRLRSPRDAIQAGIMLVPEDRKQQGCFLDHSIRRNLSLP-SLKALSALGQWVD 374

Query: 120 SLDRAAMEKQARAKLSELGLMTIQNINQAVETLSGGQRQGVAVARAAAFGSKVVIMDEPT 179
                 + +  R KL     + + +   A+  LSGG +Q V + RA A   KV+I+DEPT
Sbjct: 375 ERAERDLVETYRQKLR----IKMADAETAIGKLSGGNQQKVLLGRAMALTPKVLIVDEPT 430

Query: 180 AALGVKESRRVLELILDVRRRGLPIVLISHNMPHVFEVADRIHIHRLGRRLCVINPKDYT 239
             + +     V +++ D+   G+ +V+IS  +  V  V+DRI + R G  +  ++ +  T
Sbjct: 431 RGIDIGAKAEVHQVLSDLADLGVAVVVISSELAEVMAVSDRIVVFREGVIVADLDAQTAT 490

Query: 240 MSDAVAFM 247
               +A+M
Sbjct: 491 EEGLMAYM 498


Lambda     K      H
   0.321    0.136    0.383 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 324
Number of extensions: 17
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 260
Length of database: 515
Length adjustment: 29
Effective length of query: 231
Effective length of database: 486
Effective search space:   112266
Effective search space used:   112266
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 49 (23.5 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources