Align 5-dehydro-2-deoxygluconokinase (EC 2.7.1.92); possible 5-dehydro-2-deoxyphosphogluconate aldolase DUF2090 (EC 4.1.2.29) (characterized)
to candidate CCNA_01356 CCNA_01356 5-dehydro-2-deoxygluconokinase IolC
Query= reanno::Caulo:CCNA_01356 (642 letters) >FitnessBrowser__Caulo:CCNA_01356 Length = 642 Score = 1283 bits (3320), Expect = 0.0 Identities = 642/642 (100%), Positives = 642/642 (100%) Query: 1 MAQDAKTLDLIAVGRSSVDLYGQQVGGRLEDMGSFAKYLGGSPTNTAAGGARLGLKTGLL 60 MAQDAKTLDLIAVGRSSVDLYGQQVGGRLEDMGSFAKYLGGSPTNTAAGGARLGLKTGLL Sbjct: 1 MAQDAKTLDLIAVGRSSVDLYGQQVGGRLEDMGSFAKYLGGSPTNTAAGGARLGLKTGLL 60 Query: 61 TRVGADHMGRFIREQLEREGVDVAGVLSDPDRLTALVILGIRDRVNFPLIFYRENCADMA 120 TRVGADHMGRFIREQLEREGVDVAGVLSDPDRLTALVILGIRDRVNFPLIFYRENCADMA Sbjct: 61 TRVGADHMGRFIREQLEREGVDVAGVLSDPDRLTALVILGIRDRVNFPLIFYRENCADMA 120 Query: 121 LEPSDVDEAWFAQAGAVLINGTHLSQPNVYETSLKAARAVKAAGGRVAFDIDYRPVLWGL 180 LEPSDVDEAWFAQAGAVLINGTHLSQPNVYETSLKAARAVKAAGGRVAFDIDYRPVLWGL Sbjct: 121 LEPSDVDEAWFAQAGAVLINGTHLSQPNVYETSLKAARAVKAAGGRVAFDIDYRPVLWGL 180 Query: 181 TGKDAGENRFVENQQVTAKLQEVVALCDLIVGTEEEIHILGGSTDTIAALRAIRRASDAL 240 TGKDAGENRFVENQQVTAKLQEVVALCDLIVGTEEEIHILGGSTDTIAALRAIRRASDAL Sbjct: 181 TGKDAGENRFVENQQVTAKLQEVVALCDLIVGTEEEIHILGGSTDTIAALRAIRRASDAL 240 Query: 241 LVCKRGPEGCVAFPGAIPDALDEGVSARGFKVEVFNVLGAGDAFMAGFLRGWLRHESVET 300 LVCKRGPEGCVAFPGAIPDALDEGVSARGFKVEVFNVLGAGDAFMAGFLRGWLRHESVET Sbjct: 241 LVCKRGPEGCVAFPGAIPDALDEGVSARGFKVEVFNVLGAGDAFMAGFLRGWLRHESVET 300 Query: 301 CCEWGNACGAIVVSRHGCTPAMPTWIELQAFLSERERPFRLREDAELEHIHWATTRERVY 360 CCEWGNACGAIVVSRHGCTPAMPTWIELQAFLSERERPFRLREDAELEHIHWATTRERVY Sbjct: 301 CCEWGNACGAIVVSRHGCTPAMPTWIELQAFLSERERPFRLREDAELEHIHWATTRERVY 360 Query: 361 DELTVLAIDHRSQFEDLIAETGGDAERIPDFKRLALRAVQMVAGRDETRFGMLLDGRFGF 420 DELTVLAIDHRSQFEDLIAETGGDAERIPDFKRLALRAVQMVAGRDETRFGMLLDGRFGF Sbjct: 361 DELTVLAIDHRSQFEDLIAETGGDAERIPDFKRLALRAVQMVAGRDETRFGMLLDGRFGF 420 Query: 421 EALAEAADHDYWIARPIELPKSRPLEFECSADVATELLEWPLNQVVKCLAFYHPDDESDL 480 EALAEAADHDYWIARPIELPKSRPLEFECSADVATELLEWPLNQVVKCLAFYHPDDESDL Sbjct: 421 EALAEAADHDYWIARPIELPKSRPLEFECSADVATELLEWPLNQVVKCLAFYHPDDESDL 480 Query: 481 RERQERQLLRLADACRKTRHELLLELILPRGMSSDSRTVARAIRRLYALGIRPDWWKLEP 540 RERQERQLLRLADACRKTRHELLLELILPRGMSSDSRTVARAIRRLYALGIRPDWWKLEP Sbjct: 481 RERQERQLLRLADACRKTRHELLLELILPRGMSSDSRTVARAIRRLYALGIRPDWWKLEP 540 Query: 541 LTDPDAWREIEIAIAENDPLCRGVVLLGLSAPEAELVASFEVVAPFPIVKGFAVGRTIFY 600 LTDPDAWREIEIAIAENDPLCRGVVLLGLSAPEAELVASFEVVAPFPIVKGFAVGRTIFY Sbjct: 541 LTDPDAWREIEIAIAENDPLCRGVVLLGLSAPEAELVASFEVVAPFPIVKGFAVGRTIFY 600 Query: 601 DVARAWLSNQIDDDAAVTALAAKFKVLVDAWRRLRGSVEKAA 642 DVARAWLSNQIDDDAAVTALAAKFKVLVDAWRRLRGSVEKAA Sbjct: 601 DVARAWLSNQIDDDAAVTALAAKFKVLVDAWRRLRGSVEKAA 642 Lambda K H 0.322 0.138 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1512 Number of extensions: 38 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 642 Length of database: 642 Length adjustment: 38 Effective length of query: 604 Effective length of database: 604 Effective search space: 364816 Effective search space used: 364816 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 54 (25.4 bits)
Align candidate CCNA_01356 CCNA_01356 (5-dehydro-2-deoxygluconokinase IolC)
to HMM TIGR04382 (iolC: 5-dehydro-2-deoxygluconokinase (EC 2.7.1.92))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR04382.hmm # target sequence database: /tmp/gapView.4370.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR04382 [M=309] Accession: TIGR04382 Description: myo_inos_iolC_N: 5-dehydro-2-deoxygluconokinase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.2e-128 414.7 0.1 3.7e-128 413.1 0.0 1.7 2 lcl|FitnessBrowser__Caulo:CCNA_01356 CCNA_01356 5-dehydro-2-deoxygluc Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__Caulo:CCNA_01356 CCNA_01356 5-dehydro-2-deoxygluconokinase IolC # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 413.1 0.0 3.7e-128 3.7e-128 2 309 .] 8 332 .. 7 332 .. 0.99 2 ? -1.3 0.0 0.053 0.053 149 194 .. 352 404 .. 344 413 .. 0.70 Alignments for each domain: == domain 1 score: 413.1 bits; conditional E-value: 3.7e-128 TIGR04382 2 ldlitiGRvgvDlyaqqigasledvksfakylGGspaNiavgaarlGlktalitkvgddqlGrfvreelereg 74 ldli++GR++vDly+qq+g +led+ sfakylGGsp+N+a+g arlGlkt+l+t+vg+d++Grf+re+lereg lcl|FitnessBrowser__Caulo:CCNA_01356 8 LDLIAVGRSSVDLYGQQVGGRLEDMGSFAKYLGGSPTNTAAGGARLGLKTGLLTRVGADHMGRFIREQLEREG 80 8************************************************************************ PP TIGR04382 75 vdtshvvtdkeartslvlleikdpdefpllfYRenaaDlaltvddvdeeliaeakallvsgtalskepsreav 147 vd+++v +d++++t+lv+l+i+d+ +fpl+fYRen+aD+al+++dvde+++a+a a+l++gt+ls++++ e++ lcl|FitnessBrowser__Caulo:CCNA_01356 81 VDVAGVLSDPDRLTALVILGIRDRVNFPLIFYRENCADMALEPSDVDEAWFAQAGAVLINGTHLSQPNVYETS 153 ************************************************************************* PP TIGR04382 148 lkalelakkagvkvvlDiDYRpvlWk............skeeasaalqlvlkkvdviiGteeEfeiaagekdd 208 lka+++ k+ag +v++DiDYRpvlW+ ++++++a+lq+v++++d+i+GteeE++i+ g++d+ lcl|FitnessBrowser__Caulo:CCNA_01356 154 LKAARAVKAAGGRVAFDIDYRPVLWGltgkdagenrfvENQQVTAKLQEVVALCDLIVGTEEEIHILGGSTDT 226 ************************************************************************* PP TIGR04382 209 eaaakallelgaelvvvKrGeeGslvytkd.....eeevevkgfkvevlkvlGaGDaFasgllygllegedle 276 aa++a+++++++l+v+KrG+eG++++ ++ +e v+++gfkvev++vlGaGDaF++g+l+g+l++e++e lcl|FitnessBrowser__Caulo:CCNA_01356 227 IAALRAIRRASDALLVCKRGPEGCVAFPGAipdalDEGVSARGFKVEVFNVLGAGDAFMAGFLRGWLRHESVE 299 *****************************9999**************************************** PP TIGR04382 277 kalelanAagaivvsrlscaeamptleeleefl 309 +++e++nA+gaivvsr++c++ampt+ el++fl lcl|FitnessBrowser__Caulo:CCNA_01356 300 TCCEWGNACGAIVVSRHGCTPAMPTWIELQAFL 332 ******************************997 PP == domain 2 score: -1.3 bits; conditional E-value: 0.053 TIGR04382 149 kalelakkagvkvvlDiDYRpvlWk.......skeeasaalqlvlkkvdviiG 194 a ++ + ++ +vl iD+R+++ + ++e++ +l+l+ v+++ G lcl|FitnessBrowser__Caulo:CCNA_01356 352 WATTRERVYDELTVLAIDHRSQFEDliaetggDAERIPDFKRLALRAVQMVAG 404 566778888999********987666666665555565556666666665555 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (309 nodes) Target sequences: 1 (642 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01 # Mc/sec: 11.16 // [ok]
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory