GapMind for catabolism of small carbon sources

 

Alignments for a candidate for badI in Caulobacter crescentus NA1000

Align BadI (characterized)
to candidate CCNA_00006 CCNA_00006 enoyl-CoA hydratase

Query= metacyc::MONOMER-892
         (260 letters)



>FitnessBrowser__Caulo:CCNA_00006
          Length = 262

 Score =  112 bits (280), Expect = 8e-30
 Identities = 89/263 (33%), Positives = 128/263 (48%), Gaps = 13/263 (4%)

Query: 2   QFEDLIYEIRN---GVAWIIINRPDKMNAFRGTTCDELIKALYKAGYDKDVGAIVLAGAG 58
           QF+ LI E      GV  I +NRP+ +NA       EL +AL  A  D  VG IVL G+ 
Sbjct: 3   QFQTLIVEAPESAPGVTLIRLNRPEALNALNTALLGELAQALAAAQADDSVGCIVLTGSA 62

Query: 59  DRAFCTGGD-QSTHDGNYDGRGTVGLPMEELHTAIRDVPKPVIARVQGYAIGGGNVLATI 117
            +AF  G D +   D  Y               AI    KP+IA V GYA+GGG  LA +
Sbjct: 63  -KAFAAGADIKEMSDKTYAQMFKADFFTAGAR-AIEQCRKPIIAAVAGYALGGGCELAMM 120

Query: 118 CDLTICSEKAIFGQVGPKMGSVDPGY-GTAFLARVVGEKKAREIWYMCKRYSGKEAEAMG 176
           CD  + ++ A FGQ    +G V PG  GT  L R VG+ KA ++    +    +EAE  G
Sbjct: 121 CDFILAADTAKFGQPEINLG-VAPGIGGTQRLTRFVGKSKAMDMILTGRMMGAEEAERSG 179

Query: 177 LANLCVPHDELDAEVQKWGEELCERSPTALAIAKRSFNMDTAHQAGIAGMGMYALKLYYD 236
           L +   P D L  E      ++  +SP A+A+ K    ++ A++  +        +L++ 
Sbjct: 180 LVSRIFPADSLVDETLAIAAKIAGQSPLAVAMNKEL--VEAAYETTLTTGVALERRLFHS 237

Query: 237 T---DESREGVKALQEKRKPEFR 256
               ++ +EG+ A  EKRKP F+
Sbjct: 238 LFAFEDQKEGMTAFVEKRKPLFK 260


Lambda     K      H
   0.319    0.138    0.418 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 136
Number of extensions: 8
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 260
Length of database: 262
Length adjustment: 25
Effective length of query: 235
Effective length of database: 237
Effective search space:    55695
Effective search space used:    55695
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 47 (22.7 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory