Alignments for a candidate for paaK in Caulobacter crescentus NA1000

GapMind for catabolism of small carbon sources

Alignments for a candidate for paaK in Caulobacter crescentus NA1000

Align medium-chain acyl-CoA ligase (EC 6.2.1.2); phenylacetate-CoA ligase (EC 6.2.1.30) (characterized)
to candidate CCNA_02483 CCNA_02483 4-coumarate--CoA ligase

Query= BRENDA::O74725
         (578 letters)



>FitnessBrowser__Caulo:CCNA_02483
          Length = 496

 Score =  146 bits (369), Expect = 2e-39
 Identities = 146/491 (29%), Positives = 217/491 (44%), Gaps = 67/491 (13%)

Query: 85  LAVFALNTIDSLPLFWAVHRLGGVLTPANASYSAAELTHQLLDSKAKALVTCVPLLSISL 144
           LAV A N +  + L  A  RLG +  P N   SA+EL H L++    A++       +  
Sbjct: 56  LAVLAKNQVLLVILHLACARLGAMFAPLNWRLSASEL-HALIEDADPAMI-------VGD 107

Query: 145 EAAAKAGLPKNRIYLLDVPEQLLGGVKPPAGYKSVSELTQAGKSLPPVDELRWSAGEGAR 204
           +  A AGL       LDV    +    P    ++  E                       
Sbjct: 108 DQLAAAGLDGVD---LDVLRAEIDCADPDTRARADRE----------------------- 141

Query: 205 RTAFVCYSSGTSGLPKGVMISHRNVIANTLQIKAFEQNYRDGGGTKPASTEVALGLLPQS 264
           R + + Y+SGTSG PKG ++S RN+    +           G   K     V L   P  
Sbjct: 142 RPSLILYTSGTSGRPKGALLSERNLDQTAINF---------GRLGKVTHESVFLVDAPMF 192

Query: 265 HIYALVVIGHAGAYRGDQTIVLPKFELKSYLNAIQQ--YKISALFLVPPIIIHMLGTQDV 322
           HI  L+         G   +V   FE    L  +      I+  F VP +   ML  Q  
Sbjct: 193 HIIGLITSIRPVLMHGGAILVSDGFEPARTLGRLGDPTLGITHYFCVPQMAA-MLRRQPA 251

Query: 323 CSKYDLSSVTSLFTGAAPLGMETAADFLK-LYPNILIRQGYGLTETCTVVSSTHPHDIWL 381
                L  +T++FTG AP     A D    L   I +  GYG++E  TV     P D  L
Sbjct: 252 FDASALRRLTAIFTGGAP---HPAPDIRAWLAEGIPMVDGYGMSEAGTVFGM--PADATL 306

Query: 382 -----GSSGALLPGVEARIVTPENKEITTYDSPGELVVRSPSVVLGYLNNEKATAETFV- 435
                GS+G  +P V  RIV  ++++      PGEL+++  +V  GY    + TA  F  
Sbjct: 307 IDARAGSAGLCMPPVFTRIVDEQDRDCPP-GVPGELLLKGDNVFRGYWRRPEDTARAFTE 365

Query: 436 DGWMRTGDEAVIRRSPKGIEHVFIVDRIKELIKVKGLQVAPAELEAHILAHPDVSDCAVI 495
           DGW RTGD A+         + ++VDR K++    G  V PAE+EA +  HP + +CAV+
Sbjct: 366 DGWFRTGDIALADAEG----YHWLVDRKKDMFISGGENVYPAEIEAALADHPAILECAVV 421

Query: 496 AIPDDRAGEVPKAIVVKSASAGSDESVSQALVKYVEDHKARHKWLKGGIRFVDAIPKSPS 555
            +PD R GEV   +V     A  D ++   ++ ++ED  AR+K L   +  V A+P++ S
Sbjct: 422 GVPDPRWGEVGHLVVTCREGAVLDLAL---ILSHLEDRLARYK-LPKALTLVAALPRTAS 477

Query: 556 GKILRRLIRDQ 566
           GKI + ++R++
Sbjct: 478 GKIQKTVLRER 488


Lambda     K      H
   0.316    0.134    0.392 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 687
Number of extensions: 49
Number of successful extensions: 8
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 578
Length of database: 496
Length adjustment: 35
Effective length of query: 543
Effective length of database: 461
Effective search space:   250323
Effective search space used:   250323
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources