Align 3-oxopimeloyl-CoA:CoA acetyltransferase (characterized)
to candidate CCNA_00544 CCNA_00544 acetyl-CoA acetyltransferase
Query= metacyc::MONOMER-20679 (395 letters) >FitnessBrowser__Caulo:CCNA_00544 Length = 391 Score = 279 bits (714), Expect = 9e-80 Identities = 172/403 (42%), Positives = 240/403 (59%), Gaps = 21/403 (5%) Query: 1 MTEAVIVSTARTPIGKAYRGALNATEGATLLGHAIEHAVKRAGIDPKEVEDVVMGAAMQQ 60 M+E VIVS ARTP+G ++ GAL + + L IE AV RAGI P +V++V++G +Q Sbjct: 1 MSEIVIVSAARTPVG-SFNGALASLPASELGKAVIEAAVSRAGIAPSDVDEVILGQVLQ- 58 Query: 61 GATGGNIARKALLRAGLPVTTAGTTIDRQCASGLQAIALAARSVLFDGVEIAVGGGGESI 120 A G AR+A ++AG+PV ++++ C SGL+A+ALAA+ + ++ V GG ES+ Sbjct: 59 AAAGQGPARQASVKAGIPVEAPAWSLNQLCGSGLRAVALAAQQIADGSAKVVVAGGQESM 118 Query: 121 S-------LVQNDKMNTFHAVDPALEAIKGDVYMA--MLDTAETVAKRYGISRERQDEYS 171 S L KM VD ++ D + M TAE +A R+ I+RE QD+++ Sbjct: 119 SQAPHAQNLRGGQKMGDLQFVDTMIKDGLWDAFHGYHMGQTAENIASRWQITREDQDKFA 178 Query: 172 LESQRRTAAAQQGGKFNDEIAPISTKMGVVDKATGAVSFKDITLSQDEGPRPETTAEGLA 231 + SQ R AAQ+ GKF+DEI PI+ K G D + +DE R T E + Sbjct: 179 VTSQNRAEAAQKAGKFDDEIVPITIK--------GRKG--DTIVDKDEFIRHGATIESVQ 228 Query: 232 GLKAVRGEGFTITAGNASQLSDGASATVIMSDKTAAAKGLKPLGIFRGMVSYGCEPDEMG 291 GLK V + ++TA NAS L+DGA+A V+MS + AA +GLKPL + G EP+ MG Sbjct: 229 GLKPVFNKEGSVTAANASGLNDGAAALVLMSAEEAAKRGLKPLARIASWANAGVEPEIMG 288 Query: 292 IGPVFAVPRLLKRHGLSVDDIGLWELNEAFAVQVLYCRDKLGIDPEKLNVNGGAISVGHP 351 GP+ A + L++ G SV D+ L E NEAFA Q L +LG+DP K+NVNGGAI++GHP Sbjct: 289 TGPIPASKKALEKAGWSVSDLDLVESNEAFAAQALCVVRELGLDPAKVNVNGGAIAIGHP 348 Query: 352 YGMSGARLAGHALIEGRRRKAKYAVVTMCVGGGMGSAGLFEIV 394 G SGAR+ + E +R AK + T+CVGGGMG A E V Sbjct: 349 IGASGARILTTLVHEMKRSGAKKGLATLCVGGGMGVAMCVEAV 391 Lambda K H 0.316 0.134 0.378 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 438 Number of extensions: 24 Number of successful extensions: 6 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 395 Length of database: 391 Length adjustment: 31 Effective length of query: 364 Effective length of database: 360 Effective search space: 131040 Effective search space used: 131040 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 50 (23.9 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory