Aligments for a candidate for N515DRAFT_2924 in Caulobacter crescentus NA1000

GapMind for catabolism of small carbon sources

Aligments for a candidate for N515DRAFT_2924 in Caulobacter crescentus NA1000

Align Basic amino acid/polyamine antiporter, APA family (characterized, see rationale)
to candidate CCNA_01242 CCNA_01242 amino acid permease

Query= uniprot:A0A1I1Y8J0
         (492 letters)



>lcl|FitnessBrowser__Caulo:CCNA_01242 CCNA_01242 amino acid permease
          Length = 531

 Score =  437 bits (1123), Expect = e-127
 Identities = 243/508 (47%), Positives = 320/508 (62%), Gaps = 37/508 (7%)

Query: 16  ADLPGGGAHGEATLKRALTARHLVLLGIGAIIGAGIFVITGQAAAEHAGPAIVLSFVFAG 75
           A +    AH +  LKR L   +L+ LG+GAIIGAGIFV+TGQ A+ +AGPAI+LSF+ AG
Sbjct: 13  ASIQKEAAHSQ--LKRTLGPINLMSLGVGAIIGAGIFVLTGQVASANAGPAIMLSFIVAG 70

Query: 76  IACALAALCYAEFAAMLPVSGSAYSYSYATLGEYVAWFVGWSLVLEYLFTVATVAAGWSG 135
           IACALA LCYAE A+ +PVSGSAY+Y+Y TLGE  AW +GW LVLEY    +TVA GWSG
Sbjct: 71  IACALAGLCYAELASTMPVSGSAYTYAYGTLGEVFAWIMGWLLVLEYGVAASTVAVGWSG 130

Query: 136 YFNKLLALIS------GWIGHDVSLPQT-LAAAPFTVVDGHIQATGMFINLPAVAIIAAI 188
           Y    L  +          G D  +  T L  A      G + A    +NL A   IA +
Sbjct: 131 YVVSTLHALGINFPMIQVAGADAPMWATPLIQAVAAPGGGTMFAMTGTLNLVAAIGIAMV 190

Query: 189 TGLCYVGITQSAFVNSIIVAIKVTVILLFIAFATKYINPDNWHPFIPASEG-ASKYGWAG 247
           + L  VG+++SA VN+ IV IKV V++ FIA   +YINP NWHPFIP   G   ++G  G
Sbjct: 191 SALLVVGVSESANVNNAIVVIKVIVLVTFIAVGAQYINPANWHPFIPEPTGQPGEFGIGG 250

Query: 248 VGRAAAIVFFSYIGFDAVSTAAGEAKNPQRDMPIGIIGSLILCTILYIIVAGILTGIADF 307
           + R AAI+FF+Y+GF+AVSTAA EAKNP RD+PIGI+G+LI+CT++Y+ VA ++TG+  F
Sbjct: 251 IFRGAAIIFFAYVGFEAVSTAAAEAKNPSRDVPIGILGALIICTLIYMAVAAVMTGVVPF 310

Query: 308 RLLGTPEPVSTALDNYPSLHW---------------LQIIVVIGAVTGLSSVMLVMLMGQ 352
           R L +P P++ A+D    L W               L   + IGA+TGLSSVMLV+  GQ
Sbjct: 311 RELASPAPIAVAIDRM-GLEWADIPYAAAEGGKLNLLSFAIKIGAITGLSSVMLVLCYGQ 369

Query: 353 PRIFYSMARDGLIPAVFGRIHQKFRTPHVGTVVVGVLAAALGGLFNIGVLGEMVAMGTLL 412
            RIFY+MARDGL+P VF  IH KFRTP +GT+++GV+ A       I +LG++V++GT +
Sbjct: 370 TRIFYTMARDGLLPKVFAEIHPKFRTPWLGTILLGVVIAIAASFLPISLLGDLVSLGTAV 429

Query: 413 AFATVCIGVLVLRYTRPELPRAFRVPVPWIVCPLGALACMALFLQSF---LEH------- 462
           AF+ VC+ V+ LR   P+LPR F+VP        G  AC+ L  Q+F   + H       
Sbjct: 430 AFSIVCLSVIYLRIKHPDLPRPFKVPGGIFTAAAGIAACLFLPYQNFQPMIVHAMNDNPL 489

Query: 463 -WRWMLAWIAIGQAIYFLYGYSHSKLRK 489
               +  + A+G  IY  YGY HSKL K
Sbjct: 490 PLMILGGYAAVGAIIYIAYGYWHSKLAK 517


Lambda     K      H
   0.328    0.141    0.441 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 804
Number of extensions: 55
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 492
Length of database: 531
Length adjustment: 35
Effective length of query: 457
Effective length of database: 496
Effective search space:   226672
Effective search space used:   226672
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources