GapMind for catabolism of small carbon sources

 

Aligments for a candidate for sdh in Caulobacter crescentus NA1000

Align L-iditol 2-dehydrogenase (EC 1.1.1.14) (characterized)
to candidate CCNA_01892 CCNA_01892 short chain dehydrogenase

Query= BRENDA::Q1J2J0
         (255 letters)



>lcl|FitnessBrowser__Caulo:CCNA_01892 CCNA_01892 short chain
           dehydrogenase
          Length = 546

 Score =  169 bits (429), Expect = 9e-47
 Identities = 109/254 (42%), Positives = 145/254 (57%), Gaps = 19/254 (7%)

Query: 15  RLDGRHALVTGGAQGIGFEIARGLAQAGARVTIADLNPDVGEGAARELDGTFERLNVTDA 74
           +   R  LVTGGA GIG+   +  A+AG +V +AD N +     A  L G        D 
Sbjct: 29  KAQSRVVLVTGGADGIGWAACQRFARAGDQVLVADRNVERARERADSL-GPDHHAIAMDV 87

Query: 75  DAVAD-------LARRLPDVDVLVNNAGIVRNAPAE--DTPDDDWRAVLSVNLDGVFWCC 125
            + A        L R    +DVLVNNAG+    P    D   ++   + ++N+ G F   
Sbjct: 88  SSEAQIREGFEQLHREFGRLDVLVNNAGVTDPQPTATLDQTAEEVARLQAINVTGAFLAA 147

Query: 126 REFGRTMLARGRGAIVSTASMSGLISNHPQPQAAYNASKAAVIHLTRSLAGEWASRGVRV 185
           RE GR M+ +G GAI++ AS +GL++     + +Y+ASKAAVI LTR+LA EWA++GVRV
Sbjct: 148 REAGRLMIEQGHGAIINLASGAGLVAL--AKRTSYSASKAAVISLTRTLACEWAAKGVRV 205

Query: 186 NAVAPGYTATPLTR----RGLETPEWRETWLKETPLGRLAEPREIAPAVLYLASDAASFV 241
           NAV PGYT T + +     GL  P      L   PLGR+ EP E+A    +LASDAAS+V
Sbjct: 206 NAVLPGYTRTQMVQDQIDAGLLDPS---IVLSRIPLGRMGEPEEMAEGAFFLASDAASYV 262

Query: 242 TGHTLVVDGGYTVW 255
            G TLVVDGGYTV+
Sbjct: 263 VGATLVVDGGYTVY 276



 Score =  122 bits (305), Expect = 2e-32
 Identities = 90/246 (36%), Positives = 135/246 (54%), Gaps = 14/246 (5%)

Query: 19  RHALVTGGAQGIGFEIARGLAQAGARVTIADLNPDVGEGAAREL--DGTFERLNVTDADA 76
           R + +TGG +GIG  +      AG R+ + + + +  +  A  L  +    + ++TD  A
Sbjct: 296 RVSAITGGGRGIGRCVVDLFHAAGDRLLVIERDAEGAKALAEALGDEHIVVQADITDVAA 355

Query: 77  V----ADLARRLPDVDVLVNNAGIVRN-APAEDTPDDDWRAVLSVNLDGVFWCCREFGRT 131
           V    A    R   +DVL+NNAG      P+ +    D+ +V  +N  G     +   R 
Sbjct: 356 VEAAFAQAQARWGRLDVLINNAGAADVFKPSLEQTAQDFTSVYDLNFSGPLATAKAAARL 415

Query: 132 MLARGRGAIVSTASMSGLISNHPQPQAAYNASKAAVIHLTRSLAGEWASRGVRVNAVAPG 191
           M ++G G IV+  S++GL    PQ + AY A+KAAV  ++RSLA EWAS G+RVN VAPG
Sbjct: 416 M-SQG-GVIVNLGSIAGL-GALPQ-RNAYCAAKAAVTMMSRSLACEWASAGIRVNTVAPG 471

Query: 192 YTATP--LTRRGLETPEWRETWLKETPLGRLAEPREIAPAVLYLASDAASFVTGHTLVVD 249
           Y  TP  L  +     ++ +   +  P+GRL +P E+A  + +LAS AAS+V G TL VD
Sbjct: 472 YIETPAVLALKSAGRAQF-DKIRRRAPIGRLGDPMEVARTIAFLASPAASYVAGATLTVD 530

Query: 250 GGYTVW 255
           GG+T +
Sbjct: 531 GGWTAF 536


Lambda     K      H
   0.319    0.134    0.408 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 433
Number of extensions: 26
Number of successful extensions: 9
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 255
Length of database: 546
Length adjustment: 30
Effective length of query: 225
Effective length of database: 516
Effective search space:   116100
Effective search space used:   116100
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 49 (23.5 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory