Alignments for a candidate for srlD in Caulobacter crescentus NA1000

GapMind for catabolism of small carbon sources

Alignments for a candidate for srlD in Caulobacter crescentus NA1000

Align Sorbitol-6-phosphate 2-dehydrogenase (EC 1.1.1.140) (characterized)
to candidate CCNA_01892 CCNA_01892 short chain dehydrogenase

Query= reanno::Koxy:BWI76_RS01745
         (267 letters)



>FitnessBrowser__Caulo:CCNA_01892
          Length = 546

 Score =  115 bits (287), Expect = 3e-30
 Identities = 80/262 (30%), Positives = 128/262 (48%), Gaps = 22/262 (8%)

Query: 8   KEKIITVTGGASGIGLAIVDELLAQGANVQMIDIH---GGDKHQSSG-NYNFWPTDISSA 63
           + +++ VTGGA GIG A        G  V + D +     ++  S G +++    D+SS 
Sbjct: 31  QSRVVLVTGGADGIGWAACQRFARAGDQVLVADRNVERARERADSLGPDHHAIAMDVSSE 90

Query: 64  SEVHKTVDHIIQRFGRIDGLVNNAGVNFPRLLVDEKAPSGRYELNEAAFEKMVNINQKGV 123
           +++ +  + + + FGR+D LVNNAGV  P+       P+   +       ++  IN  G 
Sbjct: 91  AQIREGFEQLHREFGRLDVLVNNAGVTDPQ-------PTATLDQTAEEVARLQAINVTGA 143

Query: 124 FLMSQAVARQMVKQRSGVIVNVSSESGLEGSEGQSCYAATKAALNSFTRSWSKELGKHGI 183
           FL ++   R M++Q  G I+N++S +GL     ++ Y+A+KAA+ S TR+ + E    G+
Sbjct: 144 FLAAREAGRLMIEQGHGAIINLASGAGLVALAKRTSYSASKAAVISLTRTLACEWAAKGV 203

Query: 184 RVVGVAPGILEKTGLRTPEYEEALAWTRNITVEQLREGYSKNSIPLGRSGRLTEVADFVC 243
           RV  V PG           Y         I    L      + IPLGR G   E+A+   
Sbjct: 204 RVNAVLPG-----------YTRTQMVQDQIDAGLLDPSIVLSRIPLGRMGEPEEMAEGAF 252

Query: 244 YLLSERASYMTGVTTNIAGGKT 265
           +L S+ ASY+ G T  + GG T
Sbjct: 253 FLASDAASYVVGATLVVDGGYT 274



 Score = 96.3 bits (238), Expect = 1e-24
 Identities = 84/266 (31%), Positives = 119/266 (44%), Gaps = 37/266 (13%)

Query: 10  KIITVTGGASGIGLAIVDELLAQGANVQMI--DIHG--------GDKHQSSGNYNFWPTD 59
           ++  +TGG  GIG  +VD   A G  + +I  D  G        GD+H           D
Sbjct: 296 RVSAITGGGRGIGRCVVDLFHAAGDRLLVIERDAEGAKALAEALGDEHIVV------QAD 349

Query: 60  ISSASEVHKTVDHIIQRFGRIDGLVNNAGVNFPRLLVDEKAPSGRYELNEAAFEKMVNIN 119
           I+  + V         R+GR+D L+NNAG        D   PS   E     F  + ++N
Sbjct: 350 ITDVAAVEAAFAQAQARWGRLDVLINNAGA------ADVFKPS--LEQTAQDFTSVYDLN 401

Query: 120 QKGVFLMSQAVARQMVKQRSGVIVNVSSESGLEGSEGQSCYAATKAALNSFTRSWSKELG 179
             G    ++A AR M   + GVIVN+ S +GL     ++ Y A KAA+   +RS + E  
Sbjct: 402 FSGPLATAKAAARLM--SQGGVIVNLGSIAGLGALPQRNAYCAAKAAVTMMSRSLACEWA 459

Query: 180 KHGIRVVGVAPGILEKTGLRTPEYEEALAWTRNITVEQLREGYSKNSIPLGRSGRLTEVA 239
             GIRV  VAPG +E   +       AL        +++R        P+GR G   EVA
Sbjct: 460 SAGIRVNTVAPGYIETPAVL------ALKSAGRAQFDKIR-----RRAPIGRLGDPMEVA 508

Query: 240 DFVCYLLSERASYMTGVTTNIAGGKT 265
             + +L S  ASY+ G T  + GG T
Sbjct: 509 RTIAFLASPAASYVAGATLTVDGGWT 534


Lambda     K      H
   0.315    0.132    0.378 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 362
Number of extensions: 19
Number of successful extensions: 8
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 267
Length of database: 546
Length adjustment: 30
Effective length of query: 237
Effective length of database: 516
Effective search space:   122292
Effective search space used:   122292
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources