Align Energy-coupling factor transporter ATP-binding protein EcfA1; Short=ECF transporter A component EcfA; EC 7.-.-.- (characterized, see rationale)
to candidate CCNA_03235 CCNA_03235 spermidine/putrescine transport ATP-binding protein potA
Query= uniprot:P40735 (281 letters) >FitnessBrowser__Caulo:CCNA_03235 Length = 381 Score = 125 bits (315), Expect = 1e-33 Identities = 85/227 (37%), Positives = 132/227 (58%), Gaps = 7/227 (3%) Query: 6 LISVEDIVFRYRKDAERRALDGVSLQVYEGEWLAIVGHNGSGKSTLARALNGLILPESGD 65 +I+ E++ R+ K A A+D VSL V EGE+ A++G +G GK+TL R L G P G Sbjct: 14 IITFENVTKRFGKLA---AVDNVSLTVNEGEFFALLGPSGCGKTTLLRMLAGFETPTEGR 70 Query: 66 IEVAGIQLTEESVWEVRKKIGMVFQNPDNQFVGTTVRDDVAFGLENNGVPREEMIERVDW 125 I + G ++ +V ++ + MVFQ+ F TV D+VA+GL+ + VP+ E RV Sbjct: 71 ILIDGQDIS--NVPPNKRPVNMVFQSYA-VFPHMTVADNVAYGLKVDNVPKAEREARVAE 127 Query: 126 AVKQVNMQDFLDQEPHHLSGGQKQRVAIAGVIAARPDIIILDEATSMLDPIGREEVLETV 185 A++ V + ++P LSGGQ+QRVA+A + RP +++LDE S LD RE++ + Sbjct: 128 ALELVQLGGLGGRKPDQLSGGQRQRVALARALVKRPRVLLLDEPLSALDAKLREQMRTEL 187 Query: 186 RHLKEQGMATVISITHDLNEA-AKADRIIVMNGGKKYAEGPPEEIFK 231 L+E+ T I +THD +EA A A R VM+ G P ++++ Sbjct: 188 CTLQEKVGITFIMVTHDQDEALALASRCAVMSKGLLQQVATPSDLYE 234 Lambda K H 0.316 0.135 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 226 Number of extensions: 11 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 281 Length of database: 381 Length adjustment: 28 Effective length of query: 253 Effective length of database: 353 Effective search space: 89309 Effective search space used: 89309 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 48 (23.1 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory