Align Energy-coupling factor transporter ATP-binding protein EcfA2; Short=ECF transporter A component EcfA2; EC 7.-.-.- (characterized, see rationale)
to candidate CCNA_02751 CCNA_02751 ABC transporter ATP-binding protein
Query= uniprot:P70970 (276 letters) >FitnessBrowser__Caulo:CCNA_02751 Length = 332 Score = 127 bits (319), Expect = 3e-34 Identities = 77/215 (35%), Positives = 126/215 (58%), Gaps = 5/215 (2%) Query: 9 ALYDINASIKEGSYVAVIGHTGSGKSTLLQHLNGLLKPTKGQISL-GSTVIQAGKKNKDL 67 AL ++ S+K G VIG +G+GKSTL++ +NGL P+ GQ+ + G V G L Sbjct: 18 ALSGVSLSVKAGEVFGVIGASGAGKSTLIRLINGLETPSAGQVIVDGDDVAALGVAG--L 75 Query: 68 KKLRKKVGIVFQFPEHQLFEETVLKDISFGPMNFGVKKEDAEQKAREMLQLVGLSEELLD 127 + LR++VG++FQ + L +TV ++++F G + + + E+L+ VGLS Sbjct: 76 RALRRRVGMIFQH-FNLLSGKTVAQNVAFPLKLAGRPAAEVKARTAELLERVGLSAHA-G 133 Query: 128 RSPFELSGGQMRRVAIAGVLAMDPEVLVLDEPTAGLDPRGRKEIMDMFYELHQRGNLTTI 187 + P +LSGGQ +RV IA LA +P+VL+ DE T+ LDP ++I+D+ L++ LT + Sbjct: 134 KYPAQLSGGQKQRVGIARALATNPKVLLCDEATSALDPETTEQILDLIAGLNRELGLTIV 193 Query: 188 LVTHSMEDAAAYADEMIVMHKGTIQASGSPRDLFL 222 L+TH M+ D + V+ G + G+ ++FL Sbjct: 194 LITHEMDVVRRVCDRVAVLDAGRVVEEGAVEEVFL 228 Lambda K H 0.318 0.136 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 205 Number of extensions: 10 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 276 Length of database: 332 Length adjustment: 27 Effective length of query: 249 Effective length of database: 305 Effective search space: 75945 Effective search space used: 75945 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 48 (23.1 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory