Align Methylmalonate-semialdehyde dehydrogenase [inositol] (EC 1.2.1.27) (characterized)
to candidate CCNA_01360 CCNA_01360 malonate-semialdehyde dehydrogenase IolA
Query= reanno::Caulo:CCNA_01360 (500 letters) >FitnessBrowser__Caulo:CCNA_01360 Length = 500 Score = 1000 bits (2585), Expect = 0.0 Identities = 500/500 (100%), Positives = 500/500 (100%) Query: 1 MMRDIPHFIGGQKVDGASGRFGEVFDPNTGKVQARVALASAGELNTAIANAKVAQAAWAA 60 MMRDIPHFIGGQKVDGASGRFGEVFDPNTGKVQARVALASAGELNTAIANAKVAQAAWAA Sbjct: 1 MMRDIPHFIGGQKVDGASGRFGEVFDPNTGKVQARVALASAGELNTAIANAKVAQAAWAA 60 Query: 61 TNPQRRARVMFEFKRLLEVHMDELAALLSSEHGKVIADSKGDIQRGLEVIEFACGVPHLL 120 TNPQRRARVMFEFKRLLEVHMDELAALLSSEHGKVIADSKGDIQRGLEVIEFACGVPHLL Sbjct: 61 TNPQRRARVMFEFKRLLEVHMDELAALLSSEHGKVIADSKGDIQRGLEVIEFACGVPHLL 120 Query: 121 KGEYTQGAGPGIDVYSMRQPLGVVAGITPFNFPAMIPMWMFGPAIATGNAFILKPSERDP 180 KGEYTQGAGPGIDVYSMRQPLGVVAGITPFNFPAMIPMWMFGPAIATGNAFILKPSERDP Sbjct: 121 KGEYTQGAGPGIDVYSMRQPLGVVAGITPFNFPAMIPMWMFGPAIATGNAFILKPSERDP 180 Query: 181 SVPVRLAELMIEAGLPPGVLNVVHGDKDCVEAILDHPDIKAVSFVGSSDIAQSVFQRAGA 240 SVPVRLAELMIEAGLPPGVLNVVHGDKDCVEAILDHPDIKAVSFVGSSDIAQSVFQRAGA Sbjct: 181 SVPVRLAELMIEAGLPPGVLNVVHGDKDCVEAILDHPDIKAVSFVGSSDIAQSVFQRAGA 240 Query: 241 AGKRVQAMGGAKNHGLVMPDADLDQAVADIIGAAYGSAGERCMALPVVVPVGEKTATALR 300 AGKRVQAMGGAKNHGLVMPDADLDQAVADIIGAAYGSAGERCMALPVVVPVGEKTATALR Sbjct: 241 AGKRVQAMGGAKNHGLVMPDADLDQAVADIIGAAYGSAGERCMALPVVVPVGEKTATALR 300 Query: 301 EKLVAAIGGLRVGVSTDPDAHYGPVVSAAHKARIESYIQMGVDEGAELVVDGRGFSLQGH 360 EKLVAAIGGLRVGVSTDPDAHYGPVVSAAHKARIESYIQMGVDEGAELVVDGRGFSLQGH Sbjct: 301 EKLVAAIGGLRVGVSTDPDAHYGPVVSAAHKARIESYIQMGVDEGAELVVDGRGFSLQGH 360 Query: 361 EEGFFVGPTLFDHVKPTSRSYHDEIFGPVLQMVRAESLEEGIALASRHQYGNGVAIFTRN 420 EEGFFVGPTLFDHVKPTSRSYHDEIFGPVLQMVRAESLEEGIALASRHQYGNGVAIFTRN Sbjct: 361 EEGFFVGPTLFDHVKPTSRSYHDEIFGPVLQMVRAESLEEGIALASRHQYGNGVAIFTRN 420 Query: 421 GDAAREFADQVEVGMVGINVPIPVPVAYHSFGGWKRSGFGDLNQYGMDGVRFYTRTKTVT 480 GDAAREFADQVEVGMVGINVPIPVPVAYHSFGGWKRSGFGDLNQYGMDGVRFYTRTKTVT Sbjct: 421 GDAAREFADQVEVGMVGINVPIPVPVAYHSFGGWKRSGFGDLNQYGMDGVRFYTRTKTVT 480 Query: 481 QRWPKGGAVLDQSFVIPTMR 500 QRWPKGGAVLDQSFVIPTMR Sbjct: 481 QRWPKGGAVLDQSFVIPTMR 500 Lambda K H 0.320 0.137 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1076 Number of extensions: 31 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 500 Length of database: 500 Length adjustment: 34 Effective length of query: 466 Effective length of database: 466 Effective search space: 217156 Effective search space used: 217156 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 52 (24.6 bits)
Align candidate CCNA_01360 CCNA_01360 (malonate-semialdehyde dehydrogenase IolA)
to HMM TIGR01722 (mmsA: methylmalonate-semialdehyde dehydrogenase (acylating) (EC 1.2.1.27))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR01722.hmm # target sequence database: /tmp/gapView.18792.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01722 [M=477] Accession: TIGR01722 Description: MMSDH: methylmalonate-semialdehyde dehydrogenase (acylating) Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 3.2e-206 671.5 0.7 3.6e-206 671.3 0.7 1.0 1 lcl|FitnessBrowser__Caulo:CCNA_01360 CCNA_01360 malonate-semialdehyde Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__Caulo:CCNA_01360 CCNA_01360 malonate-semialdehyde dehydrogenase IolA # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 671.3 0.7 3.6e-206 3.6e-206 2 477 .] 6 483 .. 5 483 .. 0.99 Alignments for each domain: == domain 1 score: 671.3 bits; conditional E-value: 3.6e-206 TIGR01722 2 khlidGkfvegksdkyipvsnpatnevlakvaeasaeevdaavasaretfaawaetsvaerarvllryqallk 74 h+i+G+ v+g+s+++++v +p t++v a+va asa e+++a+a a+ + aawa t+ +rarv++ ++ ll+ lcl|FitnessBrowser__Caulo:CCNA_01360 6 PHFIGGQKVDGASGRFGEVFDPNTGKVQARVALASAGELNTAIANAKVAQAAWAATNPQRRARVMFEFKRLLE 78 59*********************************************************************** PP TIGR01722 75 ehrdeiaklisaeqGktledakGdvarGlevvehacsvtslllGetvesvakdvdvysirqplGvvaGitpfn 147 h de+a l+s+e+Gk+++d+kGd+ rGlev+e+ac+v+ ll+Ge ++ +dvys+rqplGvvaGitpfn lcl|FitnessBrowser__Caulo:CCNA_01360 79 VHMDELAALLSSEHGKVIADSKGDIQRGLEVIEFACGVPHLLKGEYTQGAGPGIDVYSMRQPLGVVAGITPFN 151 ************************************************************************* PP TIGR01722 148 fpamiplwmfplaiacGntfvlkpsekvpsaavklaellseaGapdGvlnvvhGdkeavdrllehpdvkavsf 220 fpamip+wmf aia Gn+f+lkpse++ps v+lael+ eaG+p GvlnvvhGdk+ v+ +l+hpd+kavsf lcl|FitnessBrowser__Caulo:CCNA_01360 152 FPAMIPMWMFGPAIATGNAFILKPSERDPSVPVRLAELMIEAGLPPGVLNVVHGDKDCVEAILDHPDIKAVSF 224 ************************************************************************* PP TIGR01722 221 vGsvavgeyiyetgsahgkrvqalaGaknhmvvlpdadkeaaldalvgaavGaaGqrcmaisaavlvGaa..k 291 vGs+++++ +++++ a gkrvqa++Gaknh++v+pdad+++a+ +++gaa+G+aG+rcma+ ++v vG+ lcl|FitnessBrowser__Caulo:CCNA_01360 225 VGSSDIAQSVFQRAGAAGKRVQAMGGAKNHGLVMPDADLDQAVADIIGAAYGSAGERCMALPVVVPVGEKtaT 297 ********************************************************************96447 PP TIGR01722 292 elveeireraekvrvgagddpgaelGplitkqakervasliasgakeGaevlldGrgykveGyeeGnfvGitl 364 l e+++ ++ +rvg +dp a++Gp+++ ++k+r++s+i+ g++eGae+++dGrg+ ++G+eeG fvG+tl lcl|FitnessBrowser__Caulo:CCNA_01360 298 ALREKLVAAIGGLRVGVSTDPDAHYGPVVSAAHKARIESYIQMGVDEGAELVVDGRGFSLQGHEEGFFVGPTL 370 8889********************************************************************* PP TIGR01722 365 lervkpdmkiykeeifGpvlvvleadtleeaiklinespyGnGtaiftsdGaaarkfqheievGqvGvnvpip 437 +++vkp+ + y++eifGpvl +++a++lee+i+l yGnG aift++G aar+f ++evG+vG+nvpip lcl|FitnessBrowser__Caulo:CCNA_01360 371 FDHVKPTSRSYHDEIFGPVLQMVRAESLEEGIALASRHQYGNGVAIFTRNGDAAREFADQVEVGMVGINVPIP 443 ************************************************************************* PP TIGR01722 438 vplpffsftGwkdslfGdlhiyGkqGvrfytrlktvtarw 477 vp++++sf+Gwk s fGdl+ yG +Gvrfytr+ktvt rw lcl|FitnessBrowser__Caulo:CCNA_01360 444 VPVAYHSFGGWKRSGFGDLNQYGMDGVRFYTRTKTVTQRW 483 **************************************** PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (477 nodes) Target sequences: 1 (500 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.02 # Mc/sec: 8.99 // [ok]
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory