GapMind for catabolism of small carbon sources

 

Protein Echvi_0343 in Echinicola vietnamensis KMM 6221, DSM 17526

Annotation: Echvi_0343 Enoyl-CoA hydratase/carnithine racemase

Length: 258 amino acids

Source: Cola in FitnessBrowser

Candidate for 20 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
L-leucine catabolism liuC hi Methylglutaconyl-CoA hydratase (EC 4.2.1.18) (characterized) 48% 100% 239.6 3-hydroxypropionyl-CoA dehydratase (EC 4.2.1.116) 32% 133.7
L-isoleucine catabolism hpcD lo 3-hydroxypropionyl-CoA dehydratase (EC 4.2.1.116) (characterized) 32% 98% 133.7 Methylglutaconyl-CoA hydratase (EC 4.2.1.18) 48% 239.6
propionate catabolism hpcD lo 3-hydroxypropionyl-CoA dehydratase (EC 4.2.1.116) (characterized) 32% 98% 133.7 Methylglutaconyl-CoA hydratase (EC 4.2.1.18) 48% 239.6
L-threonine catabolism hpcD lo 3-hydroxypropionyl-CoA dehydratase (EC 4.2.1.116) (characterized) 32% 98% 133.7 Methylglutaconyl-CoA hydratase (EC 4.2.1.18) 48% 239.6
L-valine catabolism hpcD lo 3-hydroxypropionyl-CoA dehydratase (EC 4.2.1.116) (characterized) 32% 98% 133.7 Methylglutaconyl-CoA hydratase (EC 4.2.1.18) 48% 239.6
4-hydroxybenzoate catabolism ech lo 3-hydroxybutyryl-CoA dehydratase; EC 4.2.1.55 (characterized) 34% 98% 129.4 Methylglutaconyl-CoA hydratase (EC 4.2.1.18) 48% 239.6
L-arginine catabolism ech lo 3-hydroxybutyryl-CoA dehydratase; EC 4.2.1.55 (characterized) 34% 98% 129.4 Methylglutaconyl-CoA hydratase (EC 4.2.1.18) 48% 239.6
L-citrulline catabolism ech lo 3-hydroxybutyryl-CoA dehydratase; EC 4.2.1.55 (characterized) 34% 98% 129.4 Methylglutaconyl-CoA hydratase (EC 4.2.1.18) 48% 239.6
L-lysine catabolism ech lo 3-hydroxybutyryl-CoA dehydratase; EC 4.2.1.55 (characterized) 34% 98% 129.4 Methylglutaconyl-CoA hydratase (EC 4.2.1.18) 48% 239.6
phenylacetate catabolism ech lo 3-hydroxybutyryl-CoA dehydratase; EC 4.2.1.55 (characterized) 34% 98% 129.4 Methylglutaconyl-CoA hydratase (EC 4.2.1.18) 48% 239.6
L-phenylalanine catabolism ech lo 3-hydroxybutyryl-CoA dehydratase; EC 4.2.1.55 (characterized) 34% 98% 129.4 Methylglutaconyl-CoA hydratase (EC 4.2.1.18) 48% 239.6
L-proline catabolism ech lo 3-hydroxybutyryl-CoA dehydratase; EC 4.2.1.55 (characterized) 34% 98% 129.4 Methylglutaconyl-CoA hydratase (EC 4.2.1.18) 48% 239.6
L-valine catabolism ech lo 3-hydroxybutyryl-CoA dehydratase; EC 4.2.1.55 (characterized) 34% 98% 129.4 Methylglutaconyl-CoA hydratase (EC 4.2.1.18) 48% 239.6
L-isoleucine catabolism ech lo short chain enoyl-CoA hydratase subunit (EC 4.2.1.17) (characterized) 34% 88% 126.3 Methylglutaconyl-CoA hydratase (EC 4.2.1.18) 48% 239.6
4-hydroxybenzoate catabolism paaF lo trans-2,3-dehydroadipyl-CoA hydratase (EC 4.2.1.17) (characterized) 32% 97% 125.9 Methylglutaconyl-CoA hydratase (EC 4.2.1.18) 48% 239.6
phenylacetate catabolism paaF lo trans-2,3-dehydroadipyl-CoA hydratase (EC 4.2.1.17) (characterized) 32% 97% 125.9 Methylglutaconyl-CoA hydratase (EC 4.2.1.18) 48% 239.6
L-phenylalanine catabolism paaF lo trans-2,3-dehydroadipyl-CoA hydratase (EC 4.2.1.17) (characterized) 32% 97% 125.9 Methylglutaconyl-CoA hydratase (EC 4.2.1.18) 48% 239.6
4-hydroxybenzoate catabolism dch lo cyclohexa-1,5-dienecarbonyl-CoA hydratase (EC 4.2.1.100) (characterized) 30% 99% 112.5 Methylglutaconyl-CoA hydratase (EC 4.2.1.18) 48% 239.6
phenylacetate catabolism dch lo cyclohexa-1,5-dienecarbonyl-CoA hydratase (EC 4.2.1.100) (characterized) 30% 99% 112.5 Methylglutaconyl-CoA hydratase (EC 4.2.1.18) 48% 239.6
L-phenylalanine catabolism dch lo cyclohexa-1,5-dienecarbonyl-CoA hydratase (EC 4.2.1.100) (characterized) 30% 99% 112.5 Methylglutaconyl-CoA hydratase (EC 4.2.1.18) 48% 239.6

Sequence Analysis Tools

View Echvi_0343 at FitnessBrowser

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search PFam (including for weak hits, up to E = 1)

Predict protein localization: PSORTb (Gram negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the SEED with FIGfam search

Fitness BLAST: loading...

Sequence

MEEHVKFEQKGRIGYVILSRPEKRNALNAAMVSGLQAALDRCEAIETVKVVIIKAEGKVF
CSGADLQDIERMQDNTFDENLADSEGLKKLFLRLYTFPKVVIAQVQGHALAGGCGLVSVC
DFAYAVPTAKLGYTEVRIGFVPAMVLVFLVRKLGEGKAKELLLTGELLVAPAAKEIGLIQ
EVFEPAALDGAVQEIAERLISQNSGESMALTKKMIAAVQDLPLEEALEYAARMNAKARET
EDCKRGIAAFLGKKKIEW

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory