GapMind for catabolism of small carbon sources

 

Alignments for a candidate for fadB in Echinicola vietnamensis KMM 6221, DSM 17526

Align 3-hydroxyacyl-CoA dehydrogenase type-2; 17-beta-hydroxysteroid dehydrogenase 10; 17-beta-HSD 10; 3-hydroxy-2-methylbutyryl-CoA dehydrogenase; 3-hydroxyacyl-CoA dehydrogenase type II; Mitochondrial ribonuclease P protein 2; Mitochondrial RNase P protein 2; Scully protein; Type II HADH; EC 1.1.1.35; EC 1.1.1.51; EC 1.1.1.178 (characterized)
to candidate Echvi_2940 Echvi_2940 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Query= SwissProt::O18404
         (255 letters)



>FitnessBrowser__Cola:Echvi_2940
          Length = 251

 Score =  111 bits (277), Expect = 2e-29
 Identities = 91/269 (33%), Positives = 127/269 (47%), Gaps = 46/269 (17%)

Query: 2   IKNAVSLVTGGASGLGRATAERLAKQGASVILADLPSSKGNEVAKEL---GDKVVFVPVD 58
           +KN   L+TGGASG+G A  +R A++G +V   D     G +VA+EL   G +V F+  D
Sbjct: 1   MKNKTILITGGASGIGLAMTKRFAEEGGNVYFIDYDQKTGEKVAEELTSKGHRVTFLQGD 60

Query: 59  VTSEKDVSAALQTAKDKFGRLDLTVNCAGTATAVKTFNFNKNVAHRLEDFQRVININTVG 118
           V+  +++    QT     G +D+ VN AG +      N  +      EDF R+  +N  G
Sbjct: 61  VSQTEEMK---QTISSISGSIDVLVNNAGISHVGNLENTAE------EDFDRLYQVNVKG 111

Query: 119 TFNVIRLSAGLMGANEPNQDGQRGVIVNTASVAAFDGQIGQAAYSASKAAVVGMTLPIAR 178
            +N    S        P    + G I+N ASVA+  G   + AYS +K AV  MTL +AR
Sbjct: 112 IYNCSLASL-------PKMKEKGGSIINMASVASTMGLPDRFAYSMTKGAVFSMTLSMAR 164

Query: 179 DLSTQGIRICTIAPGLFNTPMLAALPEKVRTFLAKSIPFPQ--------------RLGEP 224
           D     IR+ +IAPG  +TP        V  FLAK+ P  +              R+G+P
Sbjct: 165 DYVEYNIRVNSIAPGRVHTPF-------VDGFLAKNYPGKEKEMFDKLAATQPIGRMGKP 217

Query: 225 SEYAHLVQAIY----ENPLLNGEVIRIDG 249
            E A    A+Y    E   L G    IDG
Sbjct: 218 EEIA--AMAVYLSSDEASFLTGGNYPIDG 244


Lambda     K      H
   0.317    0.133    0.369 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 153
Number of extensions: 8
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 255
Length of database: 251
Length adjustment: 24
Effective length of query: 231
Effective length of database: 227
Effective search space:    52437
Effective search space used:    52437
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 46 (22.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory