Alignments for a candidate for SLC1A4 in Echinicola vietnamensis KMM 6221, DSM 17526

GapMind for catabolism of small carbon sources

Alignments for a candidate for SLC1A4 in Echinicola vietnamensis KMM 6221, DSM 17526

Align neutral amino acid transporter A (characterized)
to candidate Echvi_1519 Echvi_1519 Na+/H+-dicarboxylate symporters

Query= CharProtDB::CH_091534
         (532 letters)



>FitnessBrowser__Cola:Echvi_1519
          Length = 444

 Score =  213 bits (541), Expect = 2e-59
 Identities = 148/430 (34%), Positives = 234/430 (54%), Gaps = 39/430 (9%)

Query: 52  VLAGAGLGAALRGLSLSRTQVT------YLAFPGEMLLRMLRMIILPLVVCSLVSGAASL 105
           ++ G  LG    GL + +TQ++      Y+   G + +  L+MI +PLV+ SL+ G ++L
Sbjct: 11  IIIGLVLGLVF-GLIVIKTQMSPDFTMDYIKPIGTIFINGLKMIAVPLVLASLIVGVSNL 69

Query: 106 -DASCLGRLGGIAVAYFGLTTLSASALAVALAFIIKPGSGAQTLQSSDLGLEDSGPPPVP 164
            D + LGR+GG  +  + +TT+ A  + + L  +  PG                    +P
Sbjct: 70  GDITKLGRIGGKTIGAYMMTTVIAVTVGLLLVNVFAPGKS------------------LP 111

Query: 165 KETVDSFLDLARNLFPSNLVVAAFRTYATDYKVVTQNSSSGNVTHEKIPIGTEIEGMNIL 224
            ET +  ++   +      VV    + A + K  T      ++  E + +     G ++L
Sbjct: 112 IETRERLMEQFDD------VVGDKSSQAAELKEQTPLKPLVDMVPENVFLAAADNG-SML 164

Query: 225 GLVLFALVLGVALKKLG-SEGEDLIRFFNSLNEATMVLVSWIMWYVPVGIMFLVGSKIVE 283
            +V FA+++G+AL ++  S+   +I FF+ LN+  + +V +IM   P G+  L+ S IVE
Sbjct: 165 QVVFFAIIVGIALLEIPKSKASPVIAFFDGLNDVIIKIVGYIMLIAPYGVFALMASLIVE 224

Query: 284 MK-----DIIVLVTSLGKYIFASILGHVIHGGIVLPLIYFVFTRKNPFRFLLGLLAPFAT 338
           +        I L+ +L KY    + G ++   +V P I  +FT+     F   L      
Sbjct: 225 IAGDNPDSAIELLFALLKYSLLVVAGLLLMIVLVYPTILKLFTKVKYKDFFAALRPAQLL 284

Query: 339 AFATCSSSATLPSMMKCIEENNGVDKRISRFILPIGATVNMDGAAIFQCVAAVFIAQLNN 398
           AF+T SSSATLP  MK +EE  GV + +S F+LP+GAT+NMDG  ++Q VAAVFIAQ   
Sbjct: 285 AFSTSSSSATLPVTMKQVEEEIGVSEEVSSFVLPLGATINMDGTCLYQGVAAVFIAQALG 344

Query: 399 VELNAGQIFTILVTATASSVGAAGVPAGGVLTIAIILEAIGLPTHDLPLILAVDWIVDRT 458
           ++L+  Q   I++TAT +S+G AGVP  G++ + I+LE+IG+P+  L LILA D I+D  
Sbjct: 345 LDLSLTQQLMIVLTATLASIGTAGVPGAGLIMLLIVLESIGVPSAGLALILAPDRILDMF 404

Query: 459 TTVVNVEGDA 468
            TVVNV GDA
Sbjct: 405 RTVVNVTGDA 414


Lambda     K      H
   0.320    0.136    0.390 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 405
Number of extensions: 14
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 532
Length of database: 444
Length adjustment: 34
Effective length of query: 498
Effective length of database: 410
Effective search space:   204180
Effective search space used:   204180
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources