Alignments for a candidate for nupA in Echinicola vietnamensis KMM 6221, DSM 17526

GapMind for catabolism of small carbon sources

Alignments for a candidate for nupA in Echinicola vietnamensis KMM 6221, DSM 17526

Align Purine/cytidine ABC transporter ATP-binding protein, component of General nucleoside uptake porter, NupABC/BmpA (transports all common nucleosides as well as 5-fluorocytidine, inosine, deoxyuridine and xanthosine) (Martinussen et al., 2010) (Most similar to 3.A.1.2.12). NupA is 506aas with two ABC (C) domains. NupB has 8 predicted TMSs, NupC has 9 or 10 predicted TMSs in a 4 + 1 (or 2) + 4 arrangement (characterized)
to candidate Echvi_1282 Echvi_1282 ABC-type sugar transport system, ATPase component

Query= TCDB::A2RKA7
         (506 letters)



>FitnessBrowser__Cola:Echvi_1282
          Length = 502

 Score =  298 bits (763), Expect = 3e-85
 Identities = 173/487 (35%), Positives = 278/487 (57%), Gaps = 5/487 (1%)

Query: 11  DVTKRFGDFVANDKVNLELKKGEIHALLGENGAGKSTLMNILSGLLEPSEGEVHVKGKLE 70
           ++TK F    A D V+LEL+ G + A+LGENGAGKSTLM ILSG+    +G ++  G   
Sbjct: 6   NITKEFVGVKALDDVSLELQAGRVTAILGENGAGKSTLMKILSGVYPDYKGTIYYNGDPV 65

Query: 71  NIDSPSKAANLGIGMVHQHFMLVDAFTVTENIILGNEVTKGINL-DLKTAKKKILELSER 129
              +   A   GI ++HQ   L+   ++ ENI LG E    + L D+    K+  +L  R
Sbjct: 66  KFQNTRDAQEKGINIIHQELNLIPYLSIRENIFLGREPETPMGLLDVAKMHKEAAQLLHR 125

Query: 130 YGLSVEPDALIRDISVGQQQRVEILKTLYRGADILIFDEPTAVLTPAEITELMQIMKNLI 189
             L+V+P+  +  + VGQQQ VEI K L   + ++I DEPT+ ++  E+  L  I++ L 
Sbjct: 126 LKLNVDPETPVSQLKVGQQQLVEIAKALSLESQVIIMDEPTSAISDQEVEILFGIIRALR 185

Query: 190 KEGKSIILITHKLDEIRAVADRITVIRRGKSIDTVELGDKTNQELAELMVGRSVSFITEK 249
            EGK+I  I+HKLDE+ A+ADR  V+R GK I++ E+   T + L + MVGR +      
Sbjct: 186 AEGKAIAYISHKLDELFAIADRYVVLRDGKMIESGEMEGMTEEALIQKMVGREIVIERSC 245

Query: 250 AAAQPKDVVLEIKDLNIKESRGSLK--VKGLSLDVRAGEIVGVAGIDGNGQTELVKAITG 307
           +  Q  + VL +K L +K  + + K  ++ ++ ++  GE++G+ G+ G G+TEL++A+ G
Sbjct: 246 SGRQFDETVLSVKHLTVKHPKIADKFLLQDINFELGKGEVLGIFGLMGAGRTELMEALFG 305

Query: 308 LTKVDSGSIKLHNKDITNQRPRKITEQSVGHVPEDRHRDGLVLEMTVAENIALQTYYKPP 367
           +       I L  K    Q+P++  +  +  VPEDR +DGLVL M +  N +L       
Sbjct: 306 VLPHQGAEITLAGKVHEFQKPQEAMDAGLALVPEDRKQDGLVLCMDLCTNSSLTVV--DS 363

Query: 368 MSKYGFLDYNKINSHARELMEEFDVRGAGEWVSASSLSGGNQQKAIIAREIDRNPDLLIV 427
           +   G LD  K    A++ M E  ++ +        LSGGNQQK ++A+ +   P +L++
Sbjct: 364 ILSGGLLDDKKEKGLAQKYMGELKIKASSHRQLVEKLSGGNQQKVVLAKWLATRPKVLML 423

Query: 428 SQPTRGLDVGAIEYIHKRLIQARDEGKAVLVISFELDEILNVSDRIAVIHDGQIQGIVSP 487
            +PTRG+D+ A   I+K + Q  +EG  ++V+S EL EIL VSDR+ V+ +G++   +  
Sbjct: 424 DEPTRGIDINAKNEIYKLIRQLANEGLGLIVVSSELPEILAVSDRVLVMAEGRLTANIPI 483

Query: 488 ETTTKQE 494
           +  T ++
Sbjct: 484 DAQTSED 490



 Score = 93.2 bits (230), Expect = 2e-23
 Identities = 67/247 (27%), Positives = 124/247 (50%), Gaps = 8/247 (3%)

Query: 258 VLEIKDLNIKESRGSLKVKGLSLDVRAGEIVGVAGIDGNGQTELVKAITGLTKVDSGSIK 317
           +L +K++  KE  G   +  +SL+++AG +  + G +G G++ L+K ++G+     G+I 
Sbjct: 1   MLTVKNIT-KEFVGVKALDDVSLELQAGRVTAILGENGAGKSTLMKILSGVYPDYKGTIY 59

Query: 318 LHNKDITNQRPRKITEQSVGHVPEDRHRDGLVLEMTVAENIALQTYYKPPMSKYGFLDYN 377
            +   +  Q  R   E+ +  + ++ +   L+  +++ ENI L    + PM   G LD  
Sbjct: 60  YNGDPVKFQNTRDAQEKGINIIHQELN---LIPYLSIRENIFLGREPETPM---GLLDVA 113

Query: 378 KINSHARELMEEFDVRGAGEWVSASSLSGGNQQKAIIAREIDRNPDLLIVSQPTRGLDVG 437
           K++  A +L+    +    E    S L  G QQ   IA+ +     ++I+ +PT  +   
Sbjct: 114 KMHKEAAQLLHRLKLNVDPE-TPVSQLKVGQQQLVEIAKALSLESQVIIMDEPTSAISDQ 172

Query: 438 AIEYIHKRLIQARDEGKAVLVISFELDEILNVSDRIAVIHDGQIQGIVSPETTTKQELGI 497
            +E +   +   R EGKA+  IS +LDE+  ++DR  V+ DG++      E  T++ L  
Sbjct: 173 EVEILFGIIRALRAEGKAIAYISHKLDELFAIADRYVVLRDGKMIESGEMEGMTEEALIQ 232

Query: 498 LMVGGNI 504
            MVG  I
Sbjct: 233 KMVGREI 239


Lambda     K      H
   0.315    0.135    0.365 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 646
Number of extensions: 27
Number of successful extensions: 8
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 506
Length of database: 502
Length adjustment: 34
Effective length of query: 472
Effective length of database: 468
Effective search space:   220896
Effective search space used:   220896
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources