Alignments for a candidate for fba in Echinicola vietnamensis KMM 6221, DSM 17526

GapMind for catabolism of small carbon sources

Alignments for a candidate for fba in Echinicola vietnamensis KMM 6221, DSM 17526

Align Fructose-bisphosphate aldolase class 1; Fructose-bisphosphate aldolase class I; FBP aldolase; EC 4.1.2.13 (characterized)
to candidate Echvi_3158 Echvi_3158 DhnA-type fructose-1,6-bisphosphate aldolase and related enzymes

Query= SwissProt::P0A991
         (350 letters)



>FitnessBrowser__Cola:Echvi_3158
          Length = 353

 Score =  447 bits (1149), Expect = e-130
 Identities = 214/348 (61%), Positives = 271/348 (77%)

Query: 3   DIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNNRPPAVLRNMQTLYNTGRL 62
           +I +LLG+ A   L+H C  I  D+L  P  + +D+V  ++NR P VLR++  LYN G L
Sbjct: 6   NIVELLGEKASFYLEHICEKITKDELQTPSKNSIDKVFGNSNRNPQVLRSLSQLYNHGNL 65

Query: 63  AGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAIEAGCNCVASTYGVLASVSRR 122
           AGTGYLSILPVDQG+EHSA  SF  NP YFDP+NI++LA+EAGCN VAST+GVL   +R+
Sbjct: 66  AGTGYLSILPVDQGIEHSAAYSFYKNPDYFDPENIIKLALEAGCNGVASTFGVLGLNARK 125

Query: 123 YAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMGAVAVGATIYFGSEESRRQIEEI 182
           YAH+IPF+VK+NHNE L+YPN YDQTL+  V+ A++MGA+AVGATIYFGS ES RQ++EI
Sbjct: 126 YAHKIPFIVKINHNELLTYPNKYDQTLFGKVKTAWDMGAIAVGATIYFGSAESNRQLKEI 185

Query: 183 SAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSADLTGQANHLAATIGADIVKQKMAE 242
           + AFE AH LGM T+LW Y RN AFK +  DYH +AD+TGQANHL  TI ADI+KQK+  
Sbjct: 186 AEAFEEAHNLGMATILWCYTRNEAFKTEKEDYHAAADVTGQANHLGVTIQADIIKQKLPT 245

Query: 243 NNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQLANCYMGRAGLINSGGAAGGETDLSDA 302
           NN G+K I +G  DD +Y  LT+++PIDL R Q+ANCYMG+ GLINSGG + GE+DL +A
Sbjct: 246 NNFGFKEIGFGKYDDEMYKTLTTDHPIDLCRLQVANCYMGKIGLINSGGGSKGESDLVEA 305

Query: 303 VRTAVINKRAGGMGLILGRKAFKKSMADGVKLINAVQDVYLDSKITIA 350
           + TAVINKRAGG GLI+GRKAF+K   +G++L+  VQDVYLD KI+IA
Sbjct: 306 ITTAVINKRAGGSGLIMGRKAFQKPFTEGIELLRFVQDVYLDQKISIA 353


Lambda     K      H
   0.318    0.134    0.386 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 400
Number of extensions: 9
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 350
Length of database: 353
Length adjustment: 29
Effective length of query: 321
Effective length of database: 324
Effective search space:   104004
Effective search space used:   104004
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 49 (23.5 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources