Alignments for a candidate for dopDH in Echinicola vietnamensis KMM 6221, DSM 17526

GapMind for catabolism of small carbon sources

Alignments for a candidate for dopDH in Echinicola vietnamensis KMM 6221, DSM 17526

Align α-ketoglutaric semialdehyde dehydrogenase subunit (EC 1.2.1.26) (characterized)
to candidate Echvi_3952 Echvi_3952 NAD-dependent aldehyde dehydrogenases

Query= metacyc::G1G01-1343-MONOMER
         (525 letters)



>FitnessBrowser__Cola:Echvi_3952
          Length = 478

 Score =  422 bits (1085), Expect = e-122
 Identities = 239/470 (50%), Positives = 297/470 (63%), Gaps = 6/470 (1%)

Query: 53  AAFDAYRETSLEQRAEFLEAIATQIEALGDALIDRAVIETGLPKARIQGERGRTCTQLRT 112
           AAF+ Y+ TSL +RA FL  IA Q+EA+ +ALI  A  E+ LP+ RI GE GRT  Q+R 
Sbjct: 12  AAFEQYKITSLAERAAFLRKIADQLEAIKEALIPTACEESHLPEGRITGELGRTTGQIRL 71

Query: 113 FARTVRAGEWLDVRIDSALPERQPLPRADLRQRQVALGPVAVFGASNFPLAFSVAGGDTA 172
           FA+ V  G WL+V ID   PER P P+ DLR+  V LGPVAVFGASNFPLAFS AGGD+ 
Sbjct: 72  FAKYVEEGTWLEVTIDHGDPERTPAPKPDLRRMLVPLGPVAVFGASNFPLAFSTAGGDSI 131

Query: 173 SALAAGCPVVVKAHSAHPGTSELVGQAVAQAVKQCGLPEGVFSLLYGSGREVGIALVSDP 232
           SALAAGC VV K H  HP TS +V +A+ QA+   GLPEGVF  + G G   G  LV  P
Sbjct: 132 SALAAGCTVVYKGHPGHPKTSLMVFEAIQQAIAAAGLPEGVFQHVEG-GISEGQTLVQHP 190

Query: 233 RIKAVGFTGSRSGGMALCQAAQARPEPIPVYAEMSSINPVFLFDAALQARAEALAQGFVA 292
            IKAVGFTGS  GG AL   A +RPEPIPVYAEM SINP+  F++AL A +E +A  +  
Sbjct: 191 AIKAVGFTGSFKGGKALFDLANSRPEPIPVYAEMGSINPIIAFESAL-ANSEQVAGQYAQ 249

Query: 293 SLTQGAGQFCTNPGLVIARQGPALQRFITAAAGYVQQGAAQTMLTPGIFSAYQAGIAALA 352
           SLT GAGQFCTNPG++      A + F       +   A Q ML  GI +AY   +  LA
Sbjct: 250 SLTLGAGQFCTNPGVIFVPADMA-EAFAQNTGKVLADAAGQAMLHEGIQTAYHQSLDHLA 308

Query: 353 DNPHAQAITSGQAGQGPNQCQAQLFVTQAEAFLADPALQAEVFGAASLVVACTDDEQVRQ 412
           D    + I    A    +     L +T  + ++  P+LQ EVFG   +VVA      + +
Sbjct: 309 DTGGLKWIQKAAAKDAGHPA---LALTDLDTWIKSPSLQEEVFGPFGIVVAYDSIAALLK 365

Query: 413 VAEHLEGQLTATLQLDEADIDSARALLPTLERKAGRILVNGWPTGVEVCDAMVHGGPFPA 472
            A+ L+GQLT TL   E+++    AL+ TL+ K GR+L  G PTGVEV  AM HGGPFPA
Sbjct: 366 AAKTLQGQLTITLWASESELTDQAALINTLQDKCGRLLFGGVPTGVEVGHAMQHGGPFPA 425

Query: 473 TSDARTTSVGTAAILRFLRPVCYQDVPDALLPQALKHGNPLQLRRLLDGK 522
           T+D+R+TSVG  AI RF RP  YQ+ PD LLP+ LK  NPL + R +DG+
Sbjct: 426 TTDSRSTSVGVYAIKRFARPFAYQNCPDGLLPKELKEANPLDIIRTVDGE 475


Lambda     K      H
   0.319    0.134    0.391 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 686
Number of extensions: 37
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 525
Length of database: 478
Length adjustment: 34
Effective length of query: 491
Effective length of database: 444
Effective search space:   218004
Effective search space used:   218004
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources