Align ABC transporter for L-Glutamine, L-Histidine, and other L-amino acids, ATPase component (characterized)
to candidate Echvi_1582 Echvi_1582 ABC-type antimicrobial peptide transport system, ATPase component
Query= reanno::Smeli:SMc02121 (258 letters) >FitnessBrowser__Cola:Echvi_1582 Length = 225 Score = 136 bits (342), Expect = 4e-37 Identities = 85/224 (37%), Positives = 134/224 (59%), Gaps = 13/224 (5%) Query: 19 IEITNMNKWYGD----FHVLRDINLRVMRGERIVVAGPSGSGKSTMIRCINRLEEHQKGK 74 + I N++K Y VL DINL V G+ I + GPSGSGK+T++ L+ G Sbjct: 4 LSIENVSKIYQSGSRTLTVLEDINLSVKAGDSIAIVGPSGSGKTTLLGLCAGLDSASSGS 63 Query: 75 IVVDG--IELTNDLKKIDEVRREVGMVFQHFNLFPHLTILENCTLAPIWVRKMPKKEAEQ 132 + ++G +E ++ ++ +E+G +FQ+F L P LT LEN + P+ ++K +K+A+Q Sbjct: 64 VALNGHRLEGLSEDQRAAVRSQEIGFIFQNFQLLPTLTALEN-VMVPLELKK--RKDAKQ 120 Query: 133 VAMHFLERVKIPEQALKYPGQLSGGQQQRVAIARSLCMRPKILLFDEPTSALDPEMVKEV 192 A L++V + ++ YP QLSGG+QQRV+IAR+ PKIL DEPT LD E E+ Sbjct: 121 KATELLQQVGLGDRMTHYPTQLSGGEQQRVSIARAFANEPKILFADEPTGNLDTE-TGEL 179 Query: 193 LDTMVGLAEE--GMTMICVTHEMGFARQVANRVIFMDQGQIVEQ 234 ++T++ + G T+I VTH+ A + NR+I + G+I E+ Sbjct: 180 IETLIFDLNKALGTTLILVTHDTDLAAK-TNRIIHIKGGKIQEE 222 Lambda K H 0.322 0.136 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 132 Number of extensions: 8 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 258 Length of database: 225 Length adjustment: 23 Effective length of query: 235 Effective length of database: 202 Effective search space: 47470 Effective search space used: 47470 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 46 (22.3 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory