GapMind for catabolism of small carbon sources

 

Aligments for a candidate for acdH in Echinicola vietnamensis KMM 6221, DSM 17526

Align 2-methylbutanoyl-CoA dehydrogenase (EC 1.3.8.5) (characterized)
to candidate Echvi_1473 Echvi_1473 Acyl-CoA dehydrogenases

Query= reanno::pseudo6_N2E2:Pf6N2E2_1146
         (375 letters)



>lcl|FitnessBrowser__Cola:Echvi_1473 Echvi_1473 Acyl-CoA
           dehydrogenases
          Length = 380

 Score =  327 bits (838), Expect = 3e-94
 Identities = 164/372 (44%), Positives = 238/372 (63%)

Query: 4   TEEQTQIRDMARQFAEERLKPFAAEWDREHRFPREAIDEMAELGFFGMLVPEQWGGCDTG 63
           TE Q+ I  M R F  + + PF  EWD    FP     ++ ELG  G+L+P ++GG   G
Sbjct: 7   TENQSMIAQMIRDFGAKEITPFRKEWDDHQTFPLPLFKKLGELGLMGVLIPSEYGGSGFG 66

Query: 64  YLAYAMTLEEIAAGDGACSTIMSVHNSVGCVPILKFGNDEQKAKFLTPLASGAMLGAFAL 123
           YL Y   + E+A  D      M+ HNS+    I+ FG++EQK K+L  LAS   LGA+ L
Sbjct: 67  YLEYVTAILELAKLDPGIGLSMAAHNSLCSGHIMLFGSEEQKQKYLPKLASCEFLGAWGL 126

Query: 124 TEPQAGSDASSLKTRARLEGDHYVLNGCKQFITSGQNAGVVIVFAVTDPSAGKRGISAFI 183
           TEP  GSDA+++KT A  EGD++VLNG K FIT G +  V +V A T     K G++AF+
Sbjct: 127 TEPNTGSDAANMKTTATAEGDYFVLNGAKNFITHGVSGDVAVVIARTGEVGDKHGMTAFV 186

Query: 184 VPTDSPGYSVARVEDKLGQHASDTCQILFEDLKVPVGNRLGEEGEGYKIALANLEGGRVG 243
           V   + G+   R EDKLG   S+T +++FED +V     LGE GEG+  ++  L+GGR+ 
Sbjct: 187 VEKGTEGFRGGRKEDKLGMRTSETAELIFEDCRVHKSQILGEVGEGFIQSMKVLDGGRIS 246

Query: 244 IAAQAVGMARAAFEAARDYARERSSFGKPIIEHQAVAFRLADMATQIAVARQMVHYAAAL 303
           IAA ++G+A  AFEAA  Y++ER  F KPI  +Q ++F+LADMAT++  A+ +   AA L
Sbjct: 247 IAALSLGIAEGAFEAALSYSKERQQFQKPISAYQGISFKLADMATKLEAAKLLTFKAADL 306

Query: 304 RDSGQPALVEASMAKLFASEMAEKVCSMALQTLGGYGYLNDFPLERIYRDVRVCQIYEGT 363
           ++ G+   + ++ AK +ASE+A ++ + A+Q  GGYG+  D+P+E+ YRDV++C I EGT
Sbjct: 307 KNRGEYVTLASAQAKYYASEIAVELANEAVQVYGGYGFTKDYPVEKYYRDVKLCTIGEGT 366

Query: 364 SDIQRMVISRNL 375
           S+IQ++VISR L
Sbjct: 367 SEIQKIVISREL 378


Lambda     K      H
   0.320    0.134    0.388 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 335
Number of extensions: 13
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 375
Length of database: 380
Length adjustment: 30
Effective length of query: 345
Effective length of database: 350
Effective search space:   120750
Effective search space used:   120750
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory