Align 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35); enoyl-CoA hydratase (EC 4.2.1.17) (characterized)
to candidate Echvi_1069 Echvi_1069 3-hydroxyacyl-CoA dehydrogenase
Query= BRENDA::Q0KEG0 (807 letters) >FitnessBrowser__Cola:Echvi_1069 Length = 801 Score = 490 bits (1262), Expect = e-142 Identities = 319/838 (38%), Positives = 441/838 (52%), Gaps = 77/838 (9%) Query: 6 VKKVAVLGAGVMGAQIAAHLINARVPVVLFDLPAKEG----PKNGIALR----------- 50 ++KVA+LG+GVMG++IA H N V V+L D+ KE K G++L Sbjct: 5 IQKVAILGSGVMGSRIACHFANIGVKVLLLDILPKEPNEAESKKGLSLEDKAVRNRLVNE 64 Query: 51 AIENLKKLSPAPLGIKEEAGLIQAANYEDDIALLKECDLVIEAIAERMDWKHDLYKKVAP 110 A+E K P+P+ + A IQ N++DD+ +K+ D VIE + ER+D K LY+KV Sbjct: 65 ALEKTLKSKPSPIYDRAFADRIQTGNFDDDLPKIKDYDWVIEVVVERLDIKQSLYEKVEQ 124 Query: 111 HLASHAIFATNTSGLSITALSDGFDADLKSRFCGVHFFNPPRYMHLVELIPTATTQPQIL 170 H + +NTSG+ + L +G D ++ FCG HFFNPPRY+ L+E+IP T P I+ Sbjct: 125 HRKPGTLITSNTSGIPMQMLCEGRSDDFQANFCGTHFFNPPRYLRLLEIIPGPKTDPAII 184 Query: 171 DQLEAFLTTTLGKGVVRAKDTPNFIANRVGIFSILAVFAEAEKFGIPFDVVDDLTGSKLG 230 D L + LGK V KDTP FIANR+G++++++ E + VD LTG+ +G Sbjct: 185 DFLMDYGDRHLGKETVLCKDTPAFIANRIGVYAMMSSMHIIESMKMGVSEVDKLTGTVIG 244 Query: 231 RAKSATFRTADVVGLDTMAHVIKTMQDTLHDDPFAPVYKTPAVLKGLVDAGALGQKTGAG 290 RAKSATFRT DVVGLDTM +V + L +D +K P + L + LG KTG G Sbjct: 245 RAKSATFRTMDVVGLDTMVNVANNLSKALQNDESKDKFKLPKTVAYLSEKKWLGDKTGQG 304 Query: 291 FY-----KKEGKAIKVLDAKTGQYVDAGK-KADEIVVRMLKKDAAERIKLLRESTNPQAQ 344 F+ K K +K +D +T +Y A K K + D ERIK L + Sbjct: 305 FFHMIRHKDGSKELKEIDLQTHEYKPAEKPKFKALEASKEINDLKERIKFLVNFDDEAGA 364 Query: 345 FLWAVFRDVFHYIAVYLEQIAGSAADIDLAIRWGFGWNSGPFEDWQSAGWKQV------- 397 F A F D+F Y + + +I+ ID A+ GFGW GPFE+W G K Sbjct: 365 FYRATFYDLFRYCSFRIPEISDELYRIDQAVCAGFGWEYGPFENWDILGLKDTIKKMEEA 424 Query: 398 ----AEWVKEDVEAGKALSAAPLPAWVFEGPVAENQGVHAAAGSWSPATQSFVARSKLPV 453 AEW+ E ++AG + E + + + + P Q F+ L Sbjct: 425 DQRPAEWIYEMLDAGNE------HFYRVEDGRRQYYDIPSKSYKDIPGQQEFILLDTL-- 476 Query: 454 YQRQAFRAAIKGTAAADPRKAGRTVEENDAVRIWVSEGQDDVLVVSFKSKMNTIGPDVID 513 +A I G A A G D+V+ + F +KMN++G +VI+ Sbjct: 477 ---KAANKKIWGNAGATIYDMG-----------------DEVIGLEFHTKMNSLGQEVIE 516 Query: 514 GLTRAIDLAEAGYKGLVVWQPTSLQLGAPGGPFSAGANLEAAMPAFMMGGAK---GIEPF 570 G+ AI +AE YKGLV+ G G FSAGANL FM G + I Sbjct: 517 GINTAITMAEKDYKGLVI--------GNEGANFSAGANLAM---LFMFAGDQEFDEINLM 565 Query: 571 VKRFQDGMMRVKYASVPVVSAASGIALGGGCELMLHSASRVAALETYIGLVEVGVGLVPA 630 + +FQ MMRV+Y+SVPVV A +ALGGGCE+ LH+ + A E Y+GLVE GVGL+PA Sbjct: 566 IAQFQKTMMRVRYSSVPVVVAPHNMALGGGCEMSLHADAVQAHAELYMGLVEFGVGLIPA 625 Query: 631 GGGLKEAALAAARAAQAAGSTNILQFLTSRFQSAAMAKVSASALEARQMGYLQPSDKIVF 690 GGG KE L + A +G + + L F + A AKVS SA EAR +GYLQ D I Sbjct: 626 GGGTKEMILRFSNAIH-SGDVEVNR-LQEYFMNIATAKVSTSAEEARGLGYLQAKDDITL 683 Query: 691 NVHELLYVAQNEVRALASAGYRAPL-PTLVPVAGRSGIATIKASLVNMRDGGFISTHDFL 749 N A+ +V L GY P+ T + V G++ +A +A + MR G +IS HD Sbjct: 684 NRKRQFAEAKAKVIELFDEGYTQPVQQTNIKVLGKTSLALFEAGITGMRYGAYISEHDAK 743 Query: 750 IASRIAEAVCGGDVEAGSLVSEDWLLALERKAFVDLLGTGKTQERIMGMLQTGKPVRN 807 IA ++A + GGD+ + VSE +LL LER+AF+ L G KT ERI +L GKP+RN Sbjct: 744 IARKLAWVMSGGDLSQANEVSEQYLLELEREAFLSLTGEQKTLERIQSILFKGKPLRN 801 Lambda K H 0.319 0.134 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1267 Number of extensions: 52 Number of successful extensions: 8 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 2 Number of HSP's successfully gapped: 2 Length of query: 807 Length of database: 801 Length adjustment: 41 Effective length of query: 766 Effective length of database: 760 Effective search space: 582160 Effective search space used: 582160 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 56 (26.2 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory