Alignments for a candidate for lacL in Echinicola vietnamensis KMM 6221, DSM 17526

GapMind for catabolism of small carbon sources

Alignments for a candidate for lacL in Echinicola vietnamensis KMM 6221, DSM 17526

Align β-galactosidase L+M (LacL+LacM) (EC 3.2.1.23) (characterized)
to candidate Echvi_2143 Echvi_2143 Beta-galactosidase/beta-glucuronidase

Query= CAZy::AAA25267.1
         (626 letters)



>FitnessBrowser__Cola:Echvi_2143
          Length = 1036

 Score =  337 bits (863), Expect = 2e-96
 Identities = 211/633 (33%), Positives = 326/633 (51%), Gaps = 52/633 (8%)

Query: 9   DDPEVFRVNQLPAHSDHHYYHDTAEFKTGSRFIKS----LNGAWRFNFAKTPAERPVDFY 64
           ++P V   N+LP  + +  Y + A  + G +        LNG W+F +     + P DFY
Sbjct: 30  ENPHVQEENRLPMRASYFPYENEALAEKGEKAASDRFFDLNGTWKFFWTDHYTKLPKDFY 89

Query: 65  QPDFDATDFDTIQVPGHIELAGYGQIQYINTLYPWEGKIYR-RPPYTLNQDQLTPGLFSD 123
              FD   +D  QVP + E  GYG   Y+N  +P+E  +    PP+  N+          
Sbjct: 90  ATGFDDAAWDDFQVPANWEFNGYGTPIYVN--HPFEFAVDNPNPPFIENEK--------- 138

Query: 124 AADNTVGSYLKTFDLDDVFKGQRIIIQFQGVEEALYVWLNGHFIGYSEDSFTPSEFDLTP 183
              N  G Y +   L D ++ Q++ I    V+ A  +++NG ++G  EDS   SEF+LT 
Sbjct: 139 ---NPAGVYRRKISLPDGWEDQQVFIHLGAVKSAFRLYVNGVYVGLGEDSKLESEFELTD 195

Query: 184 YIQDQGNVLAVRVYKHSTAAFIEDQDMFRFSGIFRDVNILAEPASHITDLDIRPVPNANL 243
           Y+Q   N++ + V +    +++E QD +R SGI RDV + + P  H  DL ++     + 
Sbjct: 196 YVQPGENLITIEVRRWHDGSYLEAQDFWRISGIERDVYLYSRPKVHFYDLFVKSPLTNSY 255

Query: 244 KSGELNITTKV-------TGEPATLALTVKDHDGRVLTSQTQT--------GSGSVTFDT 288
           + G+L++T  +        GE   +A  + D +G V+  QTQ         G   + F T
Sbjct: 256 RDGKLDVTVDLWNRTDEQQGENTVMA-KLLDPNGNVVFEQTQATIGLKRKQGKTVLNFAT 314

Query: 289 MLFDQLHLWSPQTPYLYQLTIEVYDADHQLLEVVPYQFGFRTVELRDDKVIYVNNKRLVI 348
            +   +  WS +TP LYQL + + +A  + LEVV    GFRT E+  ++ + VN K ++ 
Sbjct: 315 EI-PSVKAWSAETPTLYQLELVLANAAGEPLEVVRQNVGFRTYEVVGNQFL-VNGKAVLF 372

Query: 349 NGVNRHEWNAHTGRVISMADMRADIQTMLANNINADRTCHYPDQLPWYQLCDEAGIYLMA 408
            GVNRHE +  T  VI+   M  D++ M   N+NA R  HYP+   WY+LCD+ G Y++ 
Sbjct: 373 KGVNRHESHPETHHVITKEQMETDVRIMKELNMNAVRLSHYPNDPYWYELCDKYGFYVID 432

Query: 409 ETNLESHGSWQKMGAIEPSYNVPGDNPHWPAAVIDRARSNYEWFKNHPSIIFWSLGNESY 468
           E N+ESHG +       P   + G+ P W  A + R +      KNHPS++ WS+GNE+ 
Sbjct: 433 EANIESHGMY-----YSPERTL-GNAPEWLHAHMLRIKRMVFRDKNHPSVVAWSMGNEAG 486

Query: 469 AGEDIAAMQAFYKEHDDSRLVHYEGVFYTPELKDRISDVESRMYEKPQNIVAYLEDNPTK 528
            G +      + K +D SR V YE   Y     +  +D+    Y  P +++ Y E NP +
Sbjct: 487 NGYNFYKAYEWIKAYDPSRPVQYERSTY-----EWNTDIIVPQYPHPNSMIRYAESNPKR 541

Query: 529 PFLNCEYMHDMGNSLGGMQSYNDLIDKYPMYQGGFIWDFIDQALFVHDPITDQDVLRYGG 588
           P++  EY H MGNS+G  + Y ++I  YPM QGG+IWD++DQ   ++  I  + V  YGG
Sbjct: 542 PYIMSEYAHAMGNSMGNFREYWEVIKAYPMLQGGYIWDWVDQG--IYKEIDGKRVFGYGG 599

Query: 589 DF--DERHSDYAFSGNGLMFADRTPKPAMQEVK 619
           D+  +   SD  F  NG++ ADR   P   EV+
Sbjct: 600 DWGPEGTPSDDNFLINGVIMADRRWNPHAYEVR 632


Lambda     K      H
   0.320    0.138    0.430 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1921
Number of extensions: 115
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 626
Length of database: 1036
Length adjustment: 41
Effective length of query: 585
Effective length of database: 995
Effective search space:   582075
Effective search space used:   582075
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 56 (26.2 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources