Align aromatic-amino-acid transaminase (EC 2.6.1.57) (characterized)
to candidate Echvi_1046 Echvi_1046 Glutamate decarboxylase and related PLP-dependent proteins
Query= BRENDA::H7CE71 (509 letters) >FitnessBrowser__Cola:Echvi_1046 Length = 477 Score = 347 bits (889), Expect = e-100 Identities = 180/487 (36%), Positives = 278/487 (57%), Gaps = 21/487 (4%) Query: 22 LDPEEFRKQGHMVIDFIADYYQNIEKYPVLSRVEPGYLKKCLPESAPYDPEPISTILKDV 81 +D +EFRK+ H V+D++ADY + Y V V+PG + LP AP PE I +D Sbjct: 3 MDKQEFRKRAHQVVDWMADYMEQKAHYRVTPEVQPGDIFGQLPNQAPEQPESFDDIFEDF 62 Query: 82 QDHIVPGLTHWQSPNFFAYFSSTASTAGFLGEILTTGFNVVGFNWVSSPAATELENIVMD 141 ++ I+PG+THWQ P FF YF + S L E+L + +W++SPAATELE V++ Sbjct: 63 KEVILPGMTHWQHPAFFGYFPANNSEPSILAEMLMSTLGAQCMSWLTSPAATELEEKVIN 122 Query: 142 WLGDMLQLPKSFHFSGNGGGVLHGSTCEAIVCTMVAARDQM----LRRIGSENLGKLVVY 197 WL D L S+ GV+ + A +C ++AAR++ + G VY Sbjct: 123 WLRDAKGLDASWK------GVIQDTASTATLCALLAARERASGFSINAEGFSGQENYRVY 176 Query: 198 GSDQTHSTLQKATQIVGINTENFRAIKTTKSTGFALSPEMLRLTISSDLEKGLVPLFLCA 257 S+ HS++ KAT+I G+ N +K F + PE L I +DLE G P+ + + Sbjct: 177 SSEHAHSSVDKATRIAGLGLANL--VKIPVDEDFKMLPEALEEAILADLENGFTPICVVS 234 Query: 258 TIGTTATTAIDPLEALCHVAKEYGVWVHVDAAYAGSACICPEFRHFINGVEGANSFSFNP 317 +GTT++ AIDP+EA+ +A + +W H+DAAYAG+A + PEFR I G E A+S+ FNP Sbjct: 235 ALGTTSSGAIDPIEAIGKIAHRHSLWHHIDAAYAGTALLLPEFRWMIKGHELADSYVFNP 294 Query: 318 HKWLFTGMDCCCLWVKNPSVLASSLSTNPEFLRNKASDSKQVVDYKDWQIALSRRFRALK 377 HKW+FT DC L++KN + S PE+L+ D V +Y+DW I L RRFRALK Sbjct: 295 HKWMFTNFDCSVLFIKNAEAFIHTFSMTPEYLKTTQDD--HVHNYRDWGIQLGRRFRALK 352 Query: 378 LWLVLRSYGVANLRNFIRIHVNMAKTFEGLVRMDKRFEILVPRNFSLVCFRISPSALISS 437 LW+V+RS+G+ +R +R H+ + K + V ++ I P + +++CFR +L ++ Sbjct: 353 LWMVIRSFGLEGIREKLRHHLALTKMAKKRVEIEDNLIIEAPTDLNVICFRFVEKSLSTT 412 Query: 438 NEDDEIGMVNEVNCKLLEAINASGKAYMTHAVVGGLYVLRCAVGATLSEEKHIVEAWKVV 497 ++N +N L+ +N +G+ + TH V+ G YV+R +G T ++HI AW V+ Sbjct: 413 -------VLNALNQAWLQRVNQTGRVFFTHTVLDGKYVIRWVIGQTDVLQEHIDLAWTVL 465 Query: 498 QDHAKAI 504 + + + Sbjct: 466 MEELEKV 472 Lambda K H 0.321 0.135 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 652 Number of extensions: 37 Number of successful extensions: 6 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 509 Length of database: 477 Length adjustment: 34 Effective length of query: 475 Effective length of database: 443 Effective search space: 210425 Effective search space used: 210425 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 52 (24.6 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory