Aligments for a candidate for pccA1 in Echinicola vietnamensis KMM 6221, DSM 17526

GapMind for catabolism of small carbon sources

Aligments for a candidate for pccA1 in Echinicola vietnamensis KMM 6221, DSM 17526

Align acyl CoA carboxylase biotin carboxylase subunit (EC 2.1.3.15; EC 6.4.1.3; EC 6.3.4.14) (characterized)
to candidate Echvi_3962 Echvi_3962 Acetyl/propionyl-CoA carboxylase, alpha subunit

Query= metacyc::MONOMER-13597
         (509 letters)



>lcl|FitnessBrowser__Cola:Echvi_3962 Echvi_3962 Acetyl/propionyl-CoA
           carboxylase, alpha subunit
          Length = 499

 Score =  432 bits (1110), Expect = e-125
 Identities = 229/499 (45%), Positives = 317/499 (63%), Gaps = 8/499 (1%)

Query: 1   MPPFSRVLVANRGEIATRVLKAIKEMGMTAIAVYSEADKYAVHTKYADEAYYIGKAPALD 60
           MP   ++LVANRGEIA R+++ I+EMG+ ++AVYSE DK A H  YADE+Y +G AP+  
Sbjct: 1   MPKIRKILVANRGEIALRIMRTIREMGLKSVAVYSEVDKNAPHVLYADESYCLGPAPSHK 60

Query: 61  SYLNIEHIIDAAEKAHVDAIHPGYGFLSENAEFAEAVEKAGITFIGPSSEVMRKIKDKLD 120
           SYL  E II+A +    DAIHPGYGFLSEN  FA+ V  AG+ FIGPS E +  + DKL 
Sbjct: 61  SYLLGERIIEACQALGADAIHPGYGFLSENTAFAKKVADAGLIFIGPSPESIEIMGDKLA 120

Query: 121 GKRLANMAGVPTAPGSDGPVTSIDEALKLAEKIGYPIMVKAASGGGGVGITRVDNQDQLM 180
            K+  +   +P  PG+D  +  I EA K A +IGYPI++KA++GGGG G+  V ++ +  
Sbjct: 121 AKKAVSHYDIPMVPGTDHAILDIQEAKKTAVEIGYPILIKASAGGGGKGMRIVQDEGEFE 180

Query: 181 DVWERNKRLAYQAFGKADLFIEKYAVNPRHIEFQLIGDKYGNYVVAWERECTIQRRNQKL 240
           +  +R    A  AFG   +FIEKY  +PRHIE Q++ D++GNY+  +EREC++QRR+QK+
Sbjct: 181 EQMKRAVSEAQSAFGDGAVFIEKYITSPRHIEIQILADQHGNYLHLFERECSVQRRHQKV 240

Query: 241 IEEAPSPALKMEERESMFEPIIKFGKLINYFTLGTFETAFSDVSRDFYFLELNKRLQVEH 300
           IEEAPS  +  E R++M +  I   K   Y+  GT E    D + +FYFLE+N RLQVEH
Sbjct: 241 IEEAPSAVVNQEMRKAMGQAAIDVAKACQYYGAGTVE-FIVDEALNFYFLEMNTRLQVEH 299

Query: 301 PTTELIFRIDLVKLQIKLAAGEHLPFSQEDLNKRVRGTAIEYRINAEDALNNFTGSSGFV 360
           P TE+I   DLV+ QI +A G+ L F+Q+DL   + G AIE R+ AED  NNF    G +
Sbjct: 300 PVTEMITGKDLVREQIFIAEGQALSFAQDDLT--ILGHAIETRVYAEDPTNNFLPDIGKL 357

Query: 361 TYYREPTGPGVRVDSGIESGSYVPPYYDSLVSKLIVYGESREYAIQAGIRALADYKIGGI 420
             YR P GPG+RVD G   G  +P YYD +++KL+ + E R  AIQ  +RA+ DY I GI
Sbjct: 358 ATYRLPQGPGIRVDDGFREGMEIPIYYDPMIAKLVTFEEDRPKAIQKMVRAIDDYHITGI 417

Query: 421 KTTIELYKWIMQDPDFQEGKFSTSYISQ---KTDQFVKYLREQEEIKAAIAAEIQSRGLL 477
            TT+   +++M  P FQ G+F T ++ +    +     +  E+EEI A IAA +      
Sbjct: 418 STTLSFARFVMLHPAFQSGEFDTKFVEKHFAPSKLAENFSEEEEEILATIAAYLLPNA-- 475

Query: 478 RTSSTDNKGKAQSKSGWKT 496
           +  ST+  G+ Q+ S WKT
Sbjct: 476 KQPSTNVNGQDQNNSKWKT 494


Lambda     K      H
   0.317    0.135    0.385 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 603
Number of extensions: 34
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 509
Length of database: 499
Length adjustment: 34
Effective length of query: 475
Effective length of database: 465
Effective search space:   220875
Effective search space used:   220875
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources