Potential Gaps in catabolism of small carbon sources in Cupriavidus basilensis 4G11
Found 51 low-confidence and 44 medium-confidence steps on the best paths for 62 pathways.
| Pathway | Step | Best candidate | 2nd candidate |
|---|
| arabinose | araE: L-arabinose:H+ symporter | | |
| arabinose | xacB: L-arabinose 1-dehydrogenase | RR42_RS30145 | RR42_RS25125 |
| arabinose | xacC: L-arabinono-1,4-lactonase | RR42_RS30140 | RR42_RS33665 |
| arginine | artJ: L-arginine ABC transporter, periplasmic substrate-binding component ArtJ/HisJ/ArtI/AotJ/ArgT | RR42_RS29645 | RR42_RS34315 |
| arginine | rocF: arginase | RR42_RS02455 | RR42_RS14625 |
| cellobiose | mglB: glucose ABC transporter, substrate-binding component | | |
| cellobiose | mglC: glucose ABC transporter, permease component (MglC) | RR42_RS03365 | RR42_RS32890 |
| citrulline | AO353_03040: ABC transporter for L-Citrulline, ATPase component | RR42_RS29660 | RR42_RS34305 |
| citrulline | AO353_03045: ABC transporter for L-Citrulline, permease component 2 | RR42_RS29655 | RR42_RS04400 |
| citrulline | AO353_03050: ABC transporter for L-Citrulline, permease component 1 | RR42_RS29650 | RR42_RS04400 |
| citrulline | AO353_03055: ABC transporter for L-Citrulline, periplasmic substrate-binding component | RR42_RS29645 | RR42_RS31745 |
| citrulline | arcC: carbamate kinase | | |
| D-serine | cycA: D-serine:H+ symporter CycA | RR42_RS28305 | RR42_RS33495 |
| deoxyinosine | deoB: phosphopentomutase | RR42_RS13475 | |
| deoxyinosine | deoC: deoxyribose-5-phosphate aldolase | | |
| deoxyinosine | deoD: deoxyinosine phosphorylase | | |
| deoxyinosine | nupC: deoxyinosine:H+ symporter NupC | | |
| deoxyribonate | deoxyribonate-transport: 2-deoxy-D-ribonate transporter | RR42_RS25070 | RR42_RS04270 |
| deoxyribose | deoxyribonate-transport: 2-deoxy-D-ribonate transporter | RR42_RS25070 | RR42_RS04270 |
| deoxyribose | drdehyd-beta: 2-deoxy-D-ribose dehydrogenase, beta subunit | RR42_RS09125 | RR42_RS25790 |
| deoxyribose | drdehyd-cytc: 2-deoxyribose-D dehydrogenase, cytochrome c component | RR42_RS10775 | RR42_RS21615 |
| fucose | fdh: L-fucose 1-dehydrogenase | RR42_RS24995 | RR42_RS21135 |
| fucose | fucD: L-fuconate dehydratase | RR42_RS26650 | RR42_RS00275 |
| fucose | fuconolactonase: L-fucono-1,5-lactonase | | |
| fucose | fucU: L-fucose mutarotase FucU | | |
| fucose | HSERO_RS05255: ABC transporter for L-fucose, permease component | RR42_RS03365 | RR42_RS32890 |
| fucose | HSERO_RS05260: ABC transporter for L-fucose, substrate-binding component | | |
| galactose | dgoA: 2-dehydro-3-deoxy-6-phosphogalactonate aldolase | RR42_RS28865 | |
| galactose | dgoD: D-galactonate dehydratase | RR42_RS33950 | RR42_RS18585 |
| galactose | dgoK: 2-dehydro-3-deoxygalactonokinase | RR42_RS28860 | RR42_RS09470 |
| galactose | galdh: D-galactose 1-dehydrogenase (forming 1,4- or 1,5-lactones) | RR42_RS30145 | RR42_RS25125 |
| galactose | galP: galactose:H+ symporter GalP | | |
| glucosamine | nagB: glucosamine 6-phosphate deaminase (isomerizing) | RR42_RS09880 | RR42_RS01460 |
| glucosamine | SLC2A2: glucosamine transporter SLC2A2 | | |
| glucose | mglB: glucose ABC transporter, substrate-binding component | | |
| glucose | mglC: glucose ABC transporter, permease component (MglC) | RR42_RS03365 | RR42_RS32890 |
| glucose-6-P | uhpT: glucose-6-phosphate:phosphate antiporter | | |
| glycerol | glpD: glycerol 3-phosphate dehydrogenase (monomeric) | RR42_RS13815 | RR42_RS16760 |
| lactose | dgoA: 2-dehydro-3-deoxy-6-phosphogalactonate aldolase | RR42_RS28865 | |
| lactose | dgoD: D-galactonate dehydratase | RR42_RS33950 | RR42_RS18585 |
| lactose | dgoK: 2-dehydro-3-deoxygalactonokinase | RR42_RS28860 | RR42_RS09470 |
| lactose | galdh: D-galactose 1-dehydrogenase (forming 1,4- or 1,5-lactones) | RR42_RS30145 | RR42_RS25125 |
| lactose | lacP: lactose permease LacP | | |
| lactose | lacZ: lactase (homomeric) | RR42_RS30175 | RR42_RS26860 |
| lysine | patA: cadaverine aminotransferase | RR42_RS26850 | RR42_RS26160 |
| lysine | patD: 5-aminopentanal dehydrogenase | RR42_RS27780 | RR42_RS26255 |
| maltose | mglB: glucose ABC transporter, substrate-binding component | | |
| maltose | mglC: glucose ABC transporter, permease component (MglC) | RR42_RS03365 | RR42_RS32890 |
| maltose | susB: alpha-glucosidase (maltase) | | |
| mannitol | mt2d: mannitol 2-dehydrogenase | RR42_RS34965 | RR42_RS09405 |
| mannitol | PLT5: polyol transporter PLT5 | | |
| mannose | man-isomerase: D-mannose isomerase | RR42_RS28130 | |
| myoinositol | iolG: myo-inositol 2-dehydrogenase | | |
| myoinositol | iolM: 2-inosose 4-dehydrogenase | RR42_RS09405 | RR42_RS35005 |
| myoinositol | iolN: 2,4-diketo-inositol hydratase | | |
| myoinositol | iolO: 5-dehydro-L-gluconate epimerase | | |
| myoinositol | PS417_11885: myo-inositol ABC transporter, substrate-binding component | RR42_RS03370 | |
| myoinositol | PS417_11895: myo-inositol ABC transporter, permease component | RR42_RS03365 | RR42_RS32890 |
| myoinositol | uxaE: D-tagaturonate epimerase | | |
| myoinositol | uxuB: D-mannonate dehydrogenase | RR42_RS34965 | |
| NAG | nagA: N-acetylglucosamine 6-phosphate deacetylase | | |
| NAG | nagB: glucosamine 6-phosphate deaminase (isomerizing) | RR42_RS09880 | RR42_RS01460 |
| NAG | nagEcba: N-acetylglucosamine phosphotransferase system, EII-CBA components | | |
| phenylacetate | ppa: phenylacetate permease ppa | RR42_RS06400 | |
| phenylalanine | PPDCalpha: phenylpyruvate decarboxylase, alpha subunit | RR42_RS33045 | RR42_RS10655 |
| phenylalanine | PPDCbeta: phenylpyruvate decarboxylase, beta subunit | RR42_RS33050 | RR42_RS10660 |
| propionate | lctP: propionate permease | RR42_RS21280 | RR42_RS14610 |
| putrescine | patA: putrescine aminotransferase (PatA/SpuC) | RR42_RS27405 | RR42_RS01575 |
| putrescine | patD: gamma-aminobutyraldehyde dehydrogenase | RR42_RS26255 | RR42_RS21485 |
| putrescine | puuP: putrescine:H+ symporter PuuP/PlaP | | |
| rhamnose | aldA: lactaldehyde dehydrogenase | RR42_RS25010 | RR42_RS27780 |
| rhamnose | LRA1: L-rhamnofuranose dehydrogenase | RR42_RS21790 | RR42_RS33710 |
| rhamnose | LRA2: L-rhamnono-gamma-lactonase | | |
| rhamnose | LRA3: L-rhamnonate dehydratase | RR42_RS23065 | RR42_RS26650 |
| rhamnose | LRA4: 2-keto-3-deoxy-L-rhamnonate aldolase | RR42_RS26640 | RR42_RS29120 |
| rhamnose | rhaT: L-rhamnose:H+ symporter RhaT | | |
| ribose | rbsK: ribokinase | RR42_RS28860 | |
| ribose | rbsU: probable D-ribose transporter RbsU | | |
| sorbitol | sdh: sorbitol dehydrogenase | RR42_RS21790 | RR42_RS31670 |
| sorbitol | SOT: sorbitol:H+ co-transporter SOT1 or SOT2 | | |
| sucrose | ams: sucrose hydrolase (invertase) | | |
| thymidine | deoB: phosphopentomutase | RR42_RS13475 | |
| thymidine | deoC: deoxyribose-5-phosphate aldolase | | |
| thymidine | nupG: thymidine permease NupG/XapB | | |
| trehalose | mglB: glucose ABC transporter, substrate-binding component | | |
| trehalose | mglC: glucose ABC transporter, permease component (MglC) | RR42_RS03365 | RR42_RS32890 |
| tryptophan | antA: anthranilate 1,2-dioxygenase (deaminating, decarboxylating), large subunit AntA | RR42_RS23500 | |
| tryptophan | antB: anthranilate 1,2-dioxygenase (deaminating, decarboxylating), small subunit AntB | RR42_RS23495 | |
| tryptophan | antC: anthranilate 1,2-dioxygenase (deaminating, decarboxylating), electron transfer component AntC | RR42_RS23490 | RR42_RS32665 |
| xylitol | fruI: xylitol PTS, enzyme IIABC (FruI) | | |
| xylitol | x5p-reductase: D-xylulose-5-phosphate 2-reductase | RR42_RS09405 | RR42_RS35005 |
| xylose | HDOP-hydrol: 5-hydroxy-2,4-dioxopentanonate hydrolase | RR42_RS00385 | RR42_RS05150 |
| xylose | xad: D-xylonate dehydratase | RR42_RS18585 | RR42_RS33950 |
| xylose | xylF: ABC transporter for xylose, substrate binding component xylF | | |
| xylose | xylH: ABC transporter for xylose, permease component xylH | RR42_RS03365 | RR42_RS32890 |
Confidence: high confidence medium confidence low confidence
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Links
Downloads
Related tools
About GapMind
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using
ublast (a fast alternative to protein BLAST)
against a database of manually-curated proteins (most of which are experimentally characterized) or by using
HMMer with enzyme models (usually from
TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
- ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
- HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.
where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").
Otherwise, a candidate is "medium confidence" if either:
- ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
- ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
- HMMer finds a hit (regardless of coverage or other bits).
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps."
For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways.
For diverse bacteria and archaea that can utilize a carbon source, there is a complete
high-confidence catabolic pathway (including a transporter) just 38% of the time, and
there is a complete medium-confidence pathway 63% of the time.
Gaps may be due to:
- our ignorance of proteins' functions,
- omissions in the gene models,
- frame-shift errors in the genome sequence, or
- the organism lacks the pathway.
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory