GapMind for catabolism of small carbon sources

 

Protein RR42_RS06530 in Cupriavidus basilensis 4G11

Annotation: RR42_RS06530 C4-dicarboxylate ABC transporter

Length: 434 amino acids

Source: Cup4G11 in FitnessBrowser

Candidate for 6 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
L-malate catabolism dctM med C4-dicarboxylate TRAP transporter large permease protein DctM (characterized) 36% 100% 268.1 TRAP transporter DctM subunit, component of The possible disulfide 3,3'-dithiodipropionic acid (DTDP) tripartite transporter, DctMPQ 35% 252.7
fumarate catabolism dctM med C4-dicarboxylate TRAP transporter large permease protein DctM (characterized) 36% 100% 268.1 TRAP transporter DctM subunit, component of The possible disulfide 3,3'-dithiodipropionic acid (DTDP) tripartite transporter, DctMPQ 35% 252.7
succinate catabolism dctM med C4-dicarboxylate TRAP transporter large permease protein DctM (characterized) 36% 100% 268.1 TRAP transporter DctM subunit, component of The possible disulfide 3,3'-dithiodipropionic acid (DTDP) tripartite transporter, DctMPQ 35% 252.7
D-gluconate catabolism gntB lo TRAP-type large permease component (characterized, see rationale) 38% 100% 266.5 C4-dicarboxylate TRAP transporter large permease protein DctM 36% 268.1
D-glucuronate catabolism dctM lo Putative TRAP dicarboxylate transporter, DctM subunit (characterized, see rationale) 35% 99% 261.2 C4-dicarboxylate TRAP transporter large permease protein DctM 36% 268.1
2-oxoglutarate (alpha-ketoglutarate) catabolism dctM lo dicarboxylate TRAP transporter (succinate, fumarate, L-malate, and alpha-ketoglutarate), large permease component (characterized) 31% 100% 212.2 TRAP transporter DctM subunit, component of The possible disulfide 3,3'-dithiodipropionic acid (DTDP) tripartite transporter, DctMPQ 35% 252.7

Sequence Analysis Tools

View RR42_RS06530 at FitnessBrowser

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search PFam (including for weak hits, up to E = 1)

Predict protein localization: PSORTb (Gram negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the SEED with FIGfam search

Fitness BLAST: loading...

Sequence

MTLIAIILFVVFLGLMMLGVPIGVSLGLGGLVAIGLSNLDTQMFGLLAVPQNFYAGLGKY
PLLAIPMFVLVGSIFDRSGVAQRLVTFAIAIVGRGPGMLPLVAILVAMFLGGISGSGPAN
AAAVGGVMIAAMSRAGYPGAYSAAVVGAAAATDILIPPSVAFIIYSVLVPGASVPALFAA
GMIPGILAGVALIVPAVWLARKHNMGAIEAGLPRPPFWKSLREAAWGLVAPFLILGGMRA
GWFTPTEAAVVAVVYGLFVGMVIYRSISMRDLFVIFQEAAETSAVILLVVALAGIFAYAL
STLGVIDPLANAIAHSGLGEYGVLALIVALLMTVGMFLDGISIFLIFVPLLLPIANAFHW
NPVWFGVVLTLKVALGQFTPPLAVNLMVSCRIARVRMEETVPWVVWMLLAMFVAMLLVLA
FPPLATWLPDYLGY

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

Links

Downloads

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory