Align Alpha-ketoglutarate permease of the major facilitator superfamily protein (characterized, see rationale)
to candidate RR42_RS21430 RR42_RS21430 alpha-ketoglutarate permease
Query= uniprot:D8J257 (457 letters) >FitnessBrowser__Cup4G11:RR42_RS21430 Length = 436 Score = 591 bits (1524), Expect = e-173 Identities = 296/422 (70%), Positives = 348/422 (82%), Gaps = 1/422 (0%) Query: 10 PLSAAEYRKRIFAILGASSGNLVEWFDFYVYSFCAIYFAPAFFPKGDPTSQLLNTAGVFA 69 P SA E RKR++AI+ ASSGNLVEWFDFYVY+FCAIYFA +FFPK DPT+QLLNTAGVFA Sbjct: 12 PPSAEEKRKRVYAIVAASSGNLVEWFDFYVYAFCAIYFAGSFFPKSDPTAQLLNTAGVFA 71 Query: 70 AGFLMRPIGGWLFGRIADKHGRKTSMLISVLMMCGGSLAVAVMPTYATIGAWAPALLLLA 129 AGFLMRPIGGWLFGRIAD+HGRK S+LISV MMC GSL +A +PTY +IGAWAPALLL+A Sbjct: 72 AGFLMRPIGGWLFGRIADRHGRKNSLLISVTMMCCGSLLIASLPTYNSIGAWAPALLLVA 131 Query: 130 RLFQGLSVGGEYGTSATYMSEVAPNGRRGFFASFQYVTLIGGQLLAVLVLFGMQQWLTKA 189 RL QGLSVGGEYGT+ATYMSEVA G+RGFF+SFQYVTLIGGQLLAVLV+ +QQ+L +A Sbjct: 132 RLLQGLSVGGEYGTTATYMSEVALKGQRGFFSSFQYVTLIGGQLLAVLVVVVLQQFLDEA 191 Query: 190 ELMAWGWRVPFVLGAVGALVAMYLRSSLAETSSAGARKKKDAGTLKGLLQ-HKRAFLNVV 248 EL AWGWR+PFV+GA+ A+VA++LR +L ET+SA R K+AGTL L + HK AF V+ Sbjct: 192 ELKAWGWRIPFVIGAITAVVALFLRRTLHETTSAATRASKEAGTLTELFRNHKAAFFTVL 251 Query: 249 GFTAGGSLMFYTFTTYMQKYLVNTAGMDPKVANGVMTGALFVYMILQPIFGAISDKIGRR 308 G+TAGGSL+FYTFTTYMQKYLVN+AGM K A+ VMTG LF YM +QP FGA+SD+IGRR Sbjct: 252 GYTAGGSLIFYTFTTYMQKYLVNSAGMSIKTASYVMTGCLFFYMCMQPFFGALSDRIGRR 311 Query: 309 NSMLCFAFFGMVGTFPILHFLKDVSSPGVAMALAILALTIVSFYTSISGLIKAEMFPPEV 368 +ML F G + T PIL L V SP VA AL LAL IVS YTSISG++KAEMFP E+ Sbjct: 312 TNMLLFGALGTLATVPILTALGSVKSPFVAFALISLALAIVSLYTSISGIVKAEMFPTEI 371 Query: 369 RALGVGLSYAVGNAIFGGSAEFVALSLKSAGIESAFYWYVSALCLVALIISLRMPDPQRD 428 RALGVGL+YAV NAIFGGSAE+VAL LKS G ES+FYWYV+A+ ++ ++S R+P R Sbjct: 372 RALGVGLAYAVANAIFGGSAEYVALDLKSLGHESSFYWYVTAMMVIVFLVSFRLPRQARY 431 Query: 429 GH 430 H Sbjct: 432 LH 433 Lambda K H 0.325 0.138 0.418 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 726 Number of extensions: 35 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 457 Length of database: 436 Length adjustment: 33 Effective length of query: 424 Effective length of database: 403 Effective search space: 170872 Effective search space used: 170872 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 51 (24.3 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory