Alignments for a candidate for ald-dh-CoA in Cupriavidus basilensis 4G11

GapMind for catabolism of small carbon sources

Alignments for a candidate for ald-dh-CoA in Cupriavidus basilensis 4G11

Align Acetaldehyde dehydrogenase 2; EC 1.2.1.10; Acetaldehyde dehydrogenase [acetylating] 2 (uncharacterized)
to candidate RR42_RS31855 RR42_RS31855 acetaldehyde dehydrogenase

Query= curated2:Q396N9
         (299 letters)



>FitnessBrowser__Cup4G11:RR42_RS31855
          Length = 303

 Score =  509 bits (1310), Expect = e-149
 Identities = 252/292 (86%), Positives = 271/292 (92%)

Query: 1   MKKIKCALIGPGNIGTDLLYKLRRSTVLEPVWMVGVDPASDGLARAREFGLKTTDKGVDG 60
           M KIKCALIGPGNIGTDLLYKLRRST+LEPVWMVGVDPASDGLARARE GLKTTD+G+DG
Sbjct: 1   MAKIKCALIGPGNIGTDLLYKLRRSTILEPVWMVGVDPASDGLARARELGLKTTDQGLDG 60

Query: 61  LLPHVAADEIRIAFDATSAYVHRDNSDKLTALGVKMIDLTPAAIGPYCVPPVNLDAHLDS 120
           LL H+AAD+IRIAFDATSAYVHR +SD+LTA GV++IDLTPAAIGP+CVPPVNLD HL S
Sbjct: 61  LLAHLAADDIRIAFDATSAYVHRQHSDQLTARGVRVIDLTPAAIGPFCVPPVNLDEHLGS 120

Query: 121 AQTNVNMVTCGGQATIPMVYAVSRVQPVAYGEIVATVSSRSVGPGTRKNIDEFTRTTSGA 180
              NVNMVTCGGQATIPMVYAVSRVQ VAYGEIVATVSSRSVGPGTR+NIDEFTRTT+GA
Sbjct: 121 NAMNVNMVTCGGQATIPMVYAVSRVQAVAYGEIVATVSSRSVGPGTRRNIDEFTRTTAGA 180

Query: 181 IEQVGGARKGKAIIVINPAEPPLIMRDTIHCLTDGPPDVDAITASVHAMVKEVQRYVPGY 240
           IEQVGGAR+GKAIIVINPAEPPLIMRDTIHCLT+  PDVDAITASVH M+ EV++YVPGY
Sbjct: 181 IEQVGGARQGKAIIVINPAEPPLIMRDTIHCLTEDEPDVDAITASVHDMIAEVRQYVPGY 240

Query: 241 TLKNGPVFDGNRVSVFMEVEGLGDYLPKYAGNLDIMTAAAAATAERFAEQML 292
           TLKNGPVFDG RVS+FMEVEGLGDYLP+YAGNLDIMTAAAA TAER    ML
Sbjct: 241 TLKNGPVFDGKRVSIFMEVEGLGDYLPRYAGNLDIMTAAAARTAERIGAAML 292


Lambda     K      H
   0.318    0.134    0.391 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 389
Number of extensions: 8
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 299
Length of database: 303
Length adjustment: 27
Effective length of query: 272
Effective length of database: 276
Effective search space:    75072
Effective search space used:    75072
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 48 (23.1 bits)

Align candidate RR42_RS31855 RR42_RS31855 (acetaldehyde dehydrogenase)
to HMM TIGR03215 (acetaldehyde dehydrogenase (acetylating) (EC 1.2.1.10))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.carbon/TIGR03215.hmm
# target sequence database:        /tmp/gapView.14169.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR03215  [M=285]
Accession:   TIGR03215
Description: ac_ald_DH_ac: acetaldehyde dehydrogenase (acetylating)
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   1.4e-146  473.2   0.3   1.6e-146  473.1   0.3    1.0  1  lcl|FitnessBrowser__Cup4G11:RR42_RS31855  RR42_RS31855 acetaldehyde dehydr


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__Cup4G11:RR42_RS31855  RR42_RS31855 acetaldehyde dehydrogenase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  473.1   0.3  1.6e-146  1.6e-146       1     283 [.       3     291 ..       3     293 .. 0.98

  Alignments for each domain:
  == domain 1  score: 473.1 bits;  conditional E-value: 1.6e-146
                                 TIGR03215   1 kvkvaiiGsGnigtdllikllrsevlelallvGidpesdGlararelgvetsaeGvdalleee...did 66 
                                               k+k+a+iG+Gnigtdll+kl rs+ le++++vG+dp+sdGlararelg++t+++G+d+ll++    di+
  lcl|FitnessBrowser__Cup4G11:RR42_RS31855   3 KIKCALIGPGNIGTDLLYKLRRSTILEPVWMVGVDPASDGLARARELGLKTTDQGLDGLLAHLaadDIR 71 
                                               79*********************************************************9987778*** PP

                                 TIGR03215  67 ivfdatsakahaenaklleelgkividltPaavGpyvvPavnleevldak..nvnlvtCgGqatiPiva 133
                                               i+fdatsa++h++++++l++ g++vidltPaa+Gp++vP+vnl+e+l ++  nvn+vtCgGqatiP+v+
  lcl|FitnessBrowser__Cup4G11:RR42_RS31855  72 IAFDATSAYVHRQHSDQLTARGVRVIDLTPAAIGPFCVPPVNLDEHLGSNamNVNMVTCGGQATIPMVY 140
                                               **********************************************977666***************** PP

                                 TIGR03215 134 avsrvakvkyaeivasiasksaGpgtranideftettskaleqvgGakkgkaiiilnPaePpllmrdtv 202
                                               avsrv+ v+y+eiva+++s+s+Gpgtr+nideft+tt+ a+eqvgGa++gkaii++nPaePpl+mrdt+
  lcl|FitnessBrowser__Cup4G11:RR42_RS31855 141 AVSRVQAVAYGEIVATVSSRSVGPGTRRNIDEFTRTTAGAIEQVGGARQGKAIIVINPAEPPLIMRDTI 209
                                               ********************************************************************* PP

                                 TIGR03215 203 yalv.eeadeeaieasveemveevqkyvpGyrlkqevvldgekvsvlleveGagdylPkyaGnldilta 270
                                               ++l+ +e+d +ai+asv++m++ev++yvpGy+lk+ +v+dg++vs+++eveG gdylP+yaGnldi+ta
  lcl|FitnessBrowser__Cup4G11:RR42_RS31855 210 HCLTeDEPDVDAITASVHDMIAEVRQYVPGYTLKNGPVFDGKRVSIFMEVEGLGDYLPRYAGNLDIMTA 278
                                               ****899************************************************************** PP

                                 TIGR03215 271 aalavaeklaeel 283
                                               aa+++ae++ +++
  lcl|FitnessBrowser__Cup4G11:RR42_RS31855 279 AAARTAERIGAAM 291
                                               *******998776 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (285 nodes)
Target sequences:                          1  (303 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.00
# Mc/sec: 9.22
//
[ok]

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources