Alignments for a candidate for hcl in Cupriavidus basilensis 4G11

GapMind for catabolism of small carbon sources

Alignments for a candidate for hcl in Cupriavidus basilensis 4G11

Align Benzoate--CoA ligase; Benzoyl-CoA synthetase; EC 6.2.1.25 (characterized)
to candidate RR42_RS06210 RR42_RS06210 AMP-binding protein

Query= SwissProt::Q8GQN9
         (527 letters)



>FitnessBrowser__Cup4G11:RR42_RS06210
          Length = 577

 Score =  206 bits (524), Expect = 2e-57
 Identities = 162/506 (32%), Positives = 238/506 (47%), Gaps = 31/506 (6%)

Query: 49  SYTYDELALRVNRCGSALRTTLGLQPKDRVLVCVLDGIDFPTTFLGAIKGGVVPIAINTL 108
           SY+Y  +    +R   ALRT LG+   DRV + +   I+     +   + G + + ++ L
Sbjct: 62  SYSYAFIQAEADRLCRALRT-LGVARGDRVGIVMPQRIETVIAHMAIYQLGAIAMPLSML 120

Query: 109 LTESDYEYMLTDSAARVAVVSQELLPLFAPMLGKVPTLEHLVVAG---GAGEDSLAALLA 165
                  Y L  S A VA+  +  +        + P L  ++ AG   G  E    ALLA
Sbjct: 121 FGPEALAYRLQHSEACVAIADESSIDNVLAARAECPALATVIGAGAAVGRAEHDWDALLA 180

Query: 166 TGSEQFEAAPTRPDDHCFWLYSSGSTGAPKGTVHIHSDLIHTAELYARPILGIREGDVVF 225
                F A  T+ D+    +Y+SG+TG PKG +  H  LI     +          D VF
Sbjct: 181 AELPAFTAVVTKADEAAVLIYTSGTTGPPKGALIPHRALIGNLPGFVCSQNWFPHADDVF 240

Query: 226 -SAAKLFFAYGLGNGLIFPLAVGATAVLMAERPTPAAVFERLRRHQPDIFYGVPTLYASM 284
            S A   +  GL + L+  L  G T V    R +    FE + R++    +  PT    M
Sbjct: 241 WSPADWAWTGGLWDALMPTLYFGKTIVGYQGRFSAQTAFELMARYRVTNTFLFPTALKQM 300

Query: 285 LANPDCPKEG-ELRLRACTSAGEALPEDVGRRWQARFGVDILDGIGSTEMLHIF----LS 339
           +     P+   +L LRA  SAGEA+ E V    +   GV + +  G TE+ +I       
Sbjct: 301 MKTCPAPRAHYQLHLRALMSAGEAVGETVFAWCRDALGVVVNEMFGQTEINYIVGNCTAQ 360

Query: 340 NRAGDVHY----GTSGKPVPGYRLRLIDEDGAEITTAGVAGELQISG--------PSSAV 387
           N  G   +    G+ G+P PG+R+ ++D++G     AG  GE+ +          P   +
Sbjct: 361 NDDGQPGWPARPGSMGRPYPGHRVAVVDDEGRPCA-AGEDGEVAVCSTDVHGHPDPVFFL 419

Query: 388 MYWNNPEKTAATFMGE----WTRSGDKYLVNDEGYYVYAGRSDDMLKVSGIYVSPIEVES 443
            YW N + TA  ++      W R+GD   ++++GY  Y GR+DD+ K SG  + P E+E+
Sbjct: 420 GYWRNDDATAGKYLARDGLHWCRTGDLARIDEDGYLWYQGRADDVFKSSGYRIGPSEIEN 479

Query: 444 ALIAHEAVLEAAVVGWEDEDHLIKPKAFIVLKPGY----GAGEALRTDLKAHVKNLLAPY 499
            L+ H AV   AVV   D +     KAFIVL P       A  AL  +L+ HV+  LAPY
Sbjct: 480 CLLKHPAVSNCAVVPSPDPERGAVVKAFIVLVPSIVRSAQADAALVAELQQHVRGQLAPY 539

Query: 500 KYPRWIEFVDDLPKTATGKIQRFKLR 525
           +YP+ IEF+  LP T TGKIQR  LR
Sbjct: 540 EYPKVIEFIAQLPMTTTGKIQRRVLR 565


Lambda     K      H
   0.319    0.138    0.412 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 817
Number of extensions: 47
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 527
Length of database: 577
Length adjustment: 36
Effective length of query: 491
Effective length of database: 541
Effective search space:   265631
Effective search space used:   265631
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources