Align Benzoate--CoA ligase; Benzoyl-CoA synthetase; EC 6.2.1.25 (characterized)
to candidate RR42_RS35150 RR42_RS35150 4-hydroxybenzoate--CoA ligase
Query= SwissProt::Q8GQN9 (527 letters) >FitnessBrowser__Cup4G11:RR42_RS35150 Length = 523 Score = 525 bits (1352), Expect = e-153 Identities = 286/510 (56%), Positives = 345/510 (67%), Gaps = 13/510 (2%) Query: 22 YNAADDLIGRNLLAGRGGKTVYIDDAGSYTYDELALRVNRCGSALRTTLGLQPKDRVLVC 81 +N A LI N A R GKT YIDD G+ +Y ELA R+ R +AL+ + G+ ++RVL+ Sbjct: 14 FNFAQHLIACN--ARRPGKTAYIDDHGAMSYGELAERIRRVAAALQAS-GIHREERVLLL 70 Query: 82 VLDGIDFPTTFLGAIKGGVVPIAINTLLTESDYEYMLTDSAARVAVVSQELLPLFAPMLG 141 + DG D+P FLGA+ G+VP+A+NTLLT DY YML S A+ +VS LLP+ + Sbjct: 71 MHDGNDWPVCFLGAMYAGIVPVAVNTLLTADDYAYMLQHSRAQAVLVSGALLPVLQQAMA 130 Query: 142 KVPTLEHLVVAG---GAGEDSLAAL---LATGSEQFEAAPTRPDDHCFWLYSSGSTGAPK 195 + V+ G D + AL + + A T PDD FWLYSSGSTG PK Sbjct: 131 QGGHEAGTVIVSQPAGPLPDGMIALDGWIDRTAPLAAPASTSPDDPGFWLYSSGSTGRPK 190 Query: 196 GTVHIHSDLIHTAELYARPILGIREGDVVFSAAKLFFAYGLGNGLIFPLAVGATAVLMAE 255 G +H + TAELYA+P+L + E D+ FSAAKL+FAYGLGN L FPL+VGAT VLMAE Sbjct: 191 GVLHSQGNPYWTAELYAKPVLSLGEADICFSAAKLYFAYGLGNALTFPLSVGATVVLMAE 250 Query: 256 RPTPAAVFERLRRHQPDIFYGVPTLYASMLANPDCPKEGELRLRACTSAGEALPEDVGRR 315 RPTP A F R +QP +F+G PT YA MLA P P ++ LR C+SAGEALP D+G R Sbjct: 251 RPTPEATFRRWLDYQPTVFFGAPTGYAGMLAAPGLPTREQVSLRLCSSAGEALPADLGER 310 Query: 316 WQARFGVDILDGIGSTEMLHIFLSNRAGDVHYGTSGKPVPGYRLRLIDEDGAEITTAGVA 375 + A FG +I+DGIGSTEMLHIFLSNR G V YGT+G PVPGY + L DEDG + G Sbjct: 311 FTAHFGCEIIDGIGSTEMLHIFLSNRPGQVRYGTTGWPVPGYAVELRDEDGHAVPD-GDV 369 Query: 376 GELQISGPSSAVMYWNNPEKTAATFMGEWTRSGDKYLVNDEGYYVYAGRSDDMLKVSGIY 435 G+L I GPS+A+MYW N EK+ TF G WT+SGDKY N +G Y YAGRSDDMLKVSGIY Sbjct: 370 GDLYIQGPSAALMYWGNREKSRETFRGGWTKSGDKYARNADGSYSYAGRSDDMLKVSGIY 429 Query: 436 VSPIEVESALIAHEAVLEAAVVGWEDEDHLIKPKAFIVLKPGYGAGEALRTDLKAHVKNL 495 VSP EVE+ L H AVLEAAV+G D L K KAF+VLKPG E T+LKA VK Sbjct: 430 VSPFEVEATLAQHPAVLEAAVIGVPDPAGLTKTKAFVVLKPGAQVSE---TELKAFVKER 486 Query: 496 LAPYKYPRWIEFVDDLPKTATGKIQRFKLR 525 LAPYKYPR IEF+ LPKTATGKIQRF+LR Sbjct: 487 LAPYKYPRVIEFMGALPKTATGKIQRFRLR 516 Lambda K H 0.319 0.138 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 862 Number of extensions: 34 Number of successful extensions: 5 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 527 Length of database: 523 Length adjustment: 35 Effective length of query: 492 Effective length of database: 488 Effective search space: 240096 Effective search space used: 240096 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 52 (24.6 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory