Alignments for a candidate for hcrA in Cupriavidus basilensis 4G11

GapMind for catabolism of small carbon sources

Alignments for a candidate for hcrA in Cupriavidus basilensis 4G11

Align 4-hydroxybenzoyl-CoA reductase subunit alpha; 4-HBCR subunit alpha; EC 1.3.7.9 (characterized)
to candidate RR42_RS02365 RR42_RS02365 carbon monoxide dehydrogenase

Query= SwissProt::O33819
         (769 letters)



>FitnessBrowser__Cup4G11:RR42_RS02365
          Length = 793

 Score =  281 bits (719), Expect = 1e-79
 Identities = 236/777 (30%), Positives = 352/777 (45%), Gaps = 59/777 (7%)

Query: 24  VTGKAKYTADIAAPDALVGRILRSPHAHARILAIDTSAAEALEGVIAVCTGAETP----- 78
           +TG  +YT DI  P    G  LRSPHAHARI++ID + A A  GV+A+ TG +       
Sbjct: 23  LTGNGQYTDDIVLPQQSYGYFLRSPHAHARIVSIDKTEALASPGVVAIFTGDDVAADKVG 82

Query: 79  -VPFGVL-------PIAENEYP-LARDKVRYRGDPVAAVAAIDEVTAEKALALIKVDYEV 129
            +P G L       P+ E  +P LA+ K R+ GD VA V A     A+ A   I V Y+ 
Sbjct: 83  GLPCGWLIHSIDGSPMKEPAHPVLAQGKARHVGDQVALVIAETLQQAKDAAEKIDVQYDE 142

Query: 130 LPAYMTPKAAMKAGAIALHDDKPNNILREV-HAEFGDVAAAFAEADLIREKTYTFAEVNH 188
           LPA +    A  A ++ +HDD P N      H +     AAFA+A  +         +  
Sbjct: 143 LPAVVNTAHAASATSL-VHDDVPENTSYVWGHGDRAATDAAFAKAAHVTTLEIINNRLIP 201

Query: 189 VHMELNATLAEYDPVRDMLTLNTTTQVPYYVHLKVAA-CLQMDSARIRVIKPFLGGGFGA 247
             +E  A  A Y    D  T+  + Q P+   L + A  L +  +R+R+I P +GGGFG+
Sbjct: 202 NAIEPRAVNASYTRQDDSYTVYVSNQNPHVERLLMGAFVLGLPESRLRIIAPDVGGGFGS 261

Query: 248 RTEGLHFEIIAGLLARKAKGTVRLLQTREETFIAHRGRPWTEVKMKIGLKKDGKIAALAL 307
           +      ++     ++K K  ++    R E+F+          K ++ +  DGK  A+ +
Sbjct: 262 KIFLYAEDVALTWASKKIKRPIKWTAERSESFLTDAHGRDHVTKAELAMDADGKFLAMRV 321

Query: 308 EATQAGGAYAGY---GIITILYTGALMHGLYHIPAIKHDAWRVYTNTPPCGAMRGHGTVD 364
             T   GAY       + TILY   L+ G Y  PAI  +   V+TNT P  A RG G  +
Sbjct: 322 HTTANMGAYLSTFASSVPTILYA-TLLAGQYATPAIYAEVRAVFTNTAPVDAYRGAGRPE 380

Query: 365 TRAAFEALLTEMGEELGIDSLKIRQINMLPQIPYVTMYAQRVMSYGVPECLEK------V 418
                E L+     E+ +D  +IR+ N +   PY T       +     CL +      V
Sbjct: 381 ATYVVERLVEAAAREMKMDPAQIRRKNFIHDFPYATPVGLTYDTGDYEPCLARAQELADV 440

Query: 419 KAASGWEERKGKLPKGRGLGIALSHFVSGTSTPKHWTGEPHATVNL------KLDFDGGI 472
           K      E   +  K RGLG +      G + P +  G   A   L      ++   G +
Sbjct: 441 KGFPARREEAKQRGKLRGLGYSCYIEACGLA-PSNIAGALGARAGLFEVGEIRVHPTGTV 499

Query: 473 TLLTGAADIGQGSNTMASQVAAEVLGVRLSRIRVISADSALTPKDNGSYSSRVTFMVGNA 532
           T+ TG+   GQG  T  +Q+ A+ LG+ L  + V+  D+   P   G+Y SR   + G+A
Sbjct: 500 TVFTGSHSHGQGHETTFAQIVADRLGIALDAVEVVHGDTGRVPFGMGTYGSRSLSVGGSA 559

Query: 533 SISAAEELKGVLVKAAAKKLDAREEDIEVIDEMFMVSGSQDPGLSFQEVVKAAMVDSGTI 592
            + A ++++    K AA  L+A + DIE  +  F V+G+ D   +F EV   A V     
Sbjct: 560 IMKALDKIEAKAKKIAAHLLEASDADIEFKNGTFSVAGT-DRSKTFGEVALTAYV----- 613

Query: 593 TVKGTYTCPTEFQGDKKIRG---SAIGATMGFCY--AAQVVEASVDEITGKVTAHKVWVA 647
                   P  +  DK   G   +A      F Y   A + E  VD  TG+    K    
Sbjct: 614 --------PHNYPLDKLEPGLNENAFYDPTNFTYPSGAYICEVEVDPDTGESKVIKFTAV 665

Query: 648 VDVGKALNPLAVEGQTQGGVWMGMGQALSEETVY--DNGRMVHGNILDYRVPTIVESPDI 705
            D G  +NP+ VEGQ  GG+  G+GQA+ E+ VY  D+G+++ G+ +DY +P   + PD 
Sbjct: 666 DDFGNIINPMIVEGQVHGGIGQGLGQAMLEQCVYDDDSGQLLTGSYMDYAMPRAGDLPDF 725

Query: 706 EVIIVESMD-PNGPFGAKEASEGMLAGFLPAIHEAVYEA---VGVRATDFPLSPDRI 758
            V   +     + P G K   E    G  PA   A+ +A   +GV     P +P R+
Sbjct: 726 TVETAKGTPCTHNPLGVKGCGEAGAIGSPPAFINALVDALSPLGVHDIQMPATPHRV 782


Lambda     K      H
   0.317    0.134    0.386 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1464
Number of extensions: 75
Number of successful extensions: 8
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 769
Length of database: 793
Length adjustment: 41
Effective length of query: 728
Effective length of database: 752
Effective search space:   547456
Effective search space used:   547456
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 55 (25.8 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources