Align 4-hydroxybenzoate 3-monooxygenase (EC 1.14.13.2) (characterized)
to candidate RR42_RS21940 RR42_RS21940 4-hydroxybenzoate 3-monooxygenase
Query= reanno::Cup4G11:RR42_RS21940 (389 letters) >FitnessBrowser__Cup4G11:RR42_RS21940 Length = 389 Score = 779 bits (2011), Expect = 0.0 Identities = 389/389 (100%), Positives = 389/389 (100%) Query: 1 MRTQVAIIGAGPAGLLLGQLLAKAGIDAVIVEQRSPDYILGRIRAGILESVTADALERAG 60 MRTQVAIIGAGPAGLLLGQLLAKAGIDAVIVEQRSPDYILGRIRAGILESVTADALERAG Sbjct: 1 MRTQVAIIGAGPAGLLLGQLLAKAGIDAVIVEQRSPDYILGRIRAGILESVTADALERAG 60 Query: 61 VAQRLQHDGLVHHGIELSYGGERHRIDFHALIGRSVTVYGQTEVTRDLMAARQAGNAVTI 120 VAQRLQHDGLVHHGIELSYGGERHRIDFHALIGRSVTVYGQTEVTRDLMAARQAGNAVTI Sbjct: 61 VAQRLQHDGLVHHGIELSYGGERHRIDFHALIGRSVTVYGQTEVTRDLMAARQAGNAVTI 120 Query: 121 YDAQDVSVHDFDTATPMVRYRKGGQQYELRCDFIAGCDGFHGVTRRSVPEPSRRIFERVY 180 YDAQDVSVHDFDTATPMVRYRKGGQQYELRCDFIAGCDGFHGVTRRSVPEPSRRIFERVY Sbjct: 121 YDAQDVSVHDFDTATPMVRYRKGGQQYELRCDFIAGCDGFHGVTRRSVPEPSRRIFERVY 180 Query: 181 PFGWLGVLADTPPVANELIYASHERGFALCSMRSLTRSRYYVQVSADERVEDWSDQRFWD 240 PFGWLGVLADTPPVANELIYASHERGFALCSMRSLTRSRYYVQVSADERVEDWSDQRFWD Sbjct: 181 PFGWLGVLADTPPVANELIYASHERGFALCSMRSLTRSRYYVQVSADERVEDWSDQRFWD 240 Query: 241 ELRSRLDTSAAEALVTGASIEKSIAPLRSFVCEPMRFGNLFLAGDAAHIVPPTGAKGLNL 300 ELRSRLDTSAAEALVTGASIEKSIAPLRSFVCEPMRFGNLFLAGDAAHIVPPTGAKGLNL Sbjct: 241 ELRSRLDTSAAEALVTGASIEKSIAPLRSFVCEPMRFGNLFLAGDAAHIVPPTGAKGLNL 300 Query: 301 AASDVLYLADGLIARYLRDDASELDAYSQKCLRRVWKAERFSWWMTSLLHRFPDADDFAL 360 AASDVLYLADGLIARYLRDDASELDAYSQKCLRRVWKAERFSWWMTSLLHRFPDADDFAL Sbjct: 301 AASDVLYLADGLIARYLRDDASELDAYSQKCLRRVWKAERFSWWMTSLLHRFPDADDFAL 360 Query: 361 RIQQAELDYLAGSRAAQMSLAENYVGLPY 389 RIQQAELDYLAGSRAAQMSLAENYVGLPY Sbjct: 361 RIQQAELDYLAGSRAAQMSLAENYVGLPY 389 Lambda K H 0.323 0.137 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 623 Number of extensions: 12 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 389 Length of database: 389 Length adjustment: 30 Effective length of query: 359 Effective length of database: 359 Effective search space: 128881 Effective search space used: 128881 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 50 (23.9 bits)
Align candidate RR42_RS21940 RR42_RS21940 (4-hydroxybenzoate 3-monooxygenase)
to HMM TIGR02360 (pobA: 4-hydroxybenzoate 3-monooxygenase (EC 1.14.13.2))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR02360.hmm # target sequence database: /tmp/gapView.32356.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR02360 [M=390] Accession: TIGR02360 Description: pbenz_hydroxyl: 4-hydroxybenzoate 3-monooxygenase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.2e-203 662.1 0.0 1.4e-203 661.9 0.0 1.0 1 lcl|FitnessBrowser__Cup4G11:RR42_RS21940 RR42_RS21940 4-hydroxybenzoate 3 Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__Cup4G11:RR42_RS21940 RR42_RS21940 4-hydroxybenzoate 3-monooxygenase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 661.9 0.0 1.4e-203 1.4e-203 1 390 [] 1 389 [] 1 389 [] 1.00 Alignments for each domain: == domain 1 score: 661.9 bits; conditional E-value: 1.4e-203 TIGR02360 1 mktqvaiigaGpsGlllgqllhkaGidavilerksrdyvlgriraGvleqgtvdlleeagvderldreg 69 m+tqvaiigaGp+Glllgqll kaGidavi+e++s+dy+lgriraG+le++t+d le+agv++rl+++g lcl|FitnessBrowser__Cup4G11:RR42_RS21940 1 MRTQVAIIGAGPAGLLLGQLLAKAGIDAVIVEQRSPDYILGRIRAGILESVTADALERAGVAQRLQHDG 69 9******************************************************************** PP TIGR02360 70 lvheGveiafegekvrvdlkkltggksvlvyGqtevtrdlyeareaaglktvyeadevrlhdlesdrpk 138 lvh+G+e+++ ge++r+d+++l g +sv+vyGqtevtrdl+ ar+a ++ t+y+a++v +hd+++ +p lcl|FitnessBrowser__Cup4G11:RR42_RS21940 70 LVHHGIELSYGGERHRIDFHALIG-RSVTVYGQTEVTRDLMAARQAGNAVTIYDAQDVSVHDFDTATPM 137 **********************99.******************************************** PP TIGR02360 139 vtfekdgeekrldcdfiaGcdGfhGvsrksipaeklkefekvypfGwlGilsetppvsdeliysnserG 207 v ++k g++++l cdfiaGcdGfhGv+r+s+p+ ++ fe+vypfGwlG+l++tppv++eliy+ +erG lcl|FitnessBrowser__Cup4G11:RR42_RS21940 138 VRYRKGGQQYELRCDFIAGCDGFHGVTRRSVPEPSRRIFERVYPFGWLGVLADTPPVANELIYASHERG 206 ********************************************************************* PP TIGR02360 208 falcslrsetrsryyvqvsltdkvedwsddrfweelkrrldeeaaeklvtgpsieksiaplrsfvaepm 276 falcs+rs trsryyvqvs +++vedwsd+rfw+el++rld+ aae lvtg sieksiaplrsfv+epm lcl|FitnessBrowser__Cup4G11:RR42_RS21940 207 FALCSMRSLTRSRYYVQVSADERVEDWSDQRFWDELRSRLDTSAAEALVTGASIEKSIAPLRSFVCEPM 275 ********************************************************************* PP TIGR02360 277 ryGrlflaGdaahivpptGakGlnlaasdvaylyealleaykekdsaglerysakalarvwkaerfsww 345 r+G lflaGdaahivpptGakGlnlaasdv yl ++l+++y d++ l++ys+k+l+rvwkaerfsww lcl|FitnessBrowser__Cup4G11:RR42_RS21940 276 RFGNLFLAGDAAHIVPPTGAKGLNLAASDVLYLADGLIARYLRDDASELDAYSQKCLRRVWKAERFSWW 344 ********************************************************************* PP TIGR02360 346 ltsllhrfpdedefdkkiqqaeleylleseaaqktlaenyvGlpy 390 +tsllhrfpd d+f +iqqael+yl++s+aaq +laenyvGlpy lcl|FitnessBrowser__Cup4G11:RR42_RS21940 345 MTSLLHRFPDADDFALRIQQAELDYLAGSRAAQMSLAENYVGLPY 389 ********************************************9 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (390 nodes) Target sequences: 1 (389 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.02 # Mc/sec: 7.50 // [ok]
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory