GapMind for catabolism of small carbon sources

 

Aligments for a candidate for cycA in Cupriavidus basilensis 4G11

Align D-serine/D-alanine/glycine transporter (characterized)
to candidate RR42_RS14635 RR42_RS14635 proline-specific permease

Query= SwissProt::P0AAE0
         (470 letters)



>lcl|FitnessBrowser__Cup4G11:RR42_RS14635 RR42_RS14635
           proline-specific permease
          Length = 466

 Score =  362 bits (928), Expect = e-104
 Identities = 182/453 (40%), Positives = 282/453 (62%), Gaps = 4/453 (0%)

Query: 17  QSLRRNLTNRHIQLIAIGGAIGTGLFMGSGKTISLAGPSIIFVYMIIGFMLFFVMRAMGE 76
           Q L+R L+ RHI+ +A+G AIGTGLF GS   I  AGP+++  Y+I G  ++ VMRA+GE
Sbjct: 6   QGLQRGLSARHIRFMALGSAIGTGLFYGSASAIQTAGPAVLLAYVIGGAAVYMVMRALGE 65

Query: 77  LLLSNLEYKSFSDFASDLLGPWAGYFTGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDW 136
           + + N    SF  +AS  LGP AG+  GWTY F  +V  +ADV A   Y  FWFPD+  W
Sbjct: 66  MAVRNPVAGSFGQYASTGLGPLAGFLLGWTYAFEMIVVCLADVTAFGIYMGFWFPDVPRW 125

Query: 137 VASLAVIVLLLTLNLATVKMFGEMEFWFAMIKIVAIVSLIVVGLVMVAMHF-QSPTGVEA 195
           +  L ++ L+  LNL +VK+FGE+EFW +++K+ AIV++I  G+ ++   F  +  GV  
Sbjct: 126 IWVLGIVFLIGGLNLLSVKVFGELEFWLSLLKVGAIVAMIGGGVAIMLFGFGMADGGVAT 185

Query: 196 SFAHLWNDGGWFPKGLSGFFAGFQIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIR 255
              +LW  GG+ P G++G  A F + +FAF GIE++G TA E +DP++ +PRAIN++P+R
Sbjct: 186 GIHNLWTHGGFMPNGIAGVIASFAVVMFAFGGIEIIGITAGEARDPQRVIPRAINAVPLR 245

Query: 256 IIMFYVFALIVIMSVTPWSSVVPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGV 315
           I++FYV  L V+MS+ PW  +  E SPFV++F  +G+ +AA+V+N VV+++A S+ NS +
Sbjct: 246 ILLFYVLTLAVLMSLYPWHRIGSEGSPFVQIFSRLGIGSAAAVLNIVVISAAVSAINSDI 305

Query: 316 FSTSRMLFGLAQEGVAPKAFAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMI 375
           F   RML+G+A +  AP+ FA +S++ VP   +      LL GVV+ YV P  +  FT+I
Sbjct: 306 FGAGRMLYGMAVQRQAPRIFASVSRKGVPWMTVVVMAFALLAGVVLNYVMPEDV--FTLI 363

Query: 376 TTVSAILFMFVWTIILCSYLVYRKQRPHLHEKSI-YKMPLGKLMCWVCMAFFVFVVVLLT 434
            +++    ++VW +IL S +  R++       ++ + +P   +      AF +F+  +L 
Sbjct: 364 ASIATFATVWVWLMILLSQVALRRRMSRAEVAALKFPVPFWPVAPLAATAFMLFIFGVLG 423

Query: 435 LEDDTRQALLVTPLWFIALGLGWLFIGKKRAAE 467
               TR ALLV  +W + L + W   G+ R  E
Sbjct: 424 WFPQTRAALLVGAVWLLLLVVAWWCWGRPRGQE 456


Lambda     K      H
   0.329    0.141    0.440 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 669
Number of extensions: 48
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 470
Length of database: 466
Length adjustment: 33
Effective length of query: 437
Effective length of database: 433
Effective search space:   189221
Effective search space used:   189221
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory