Alignments for a candidate for nagF in Cupriavidus basilensis 4G11

GapMind for catabolism of small carbon sources

Alignments for a candidate for nagF in Cupriavidus basilensis 4G11

Align N-acetylglucosamine-specific PTS system, I, HPr, and IIA components (nagF) (characterized)
to candidate RR42_RS01795 RR42_RS01795 phosphoenolpyruvate-protein phosphotransferase

Query= reanno::pseudo5_N2C3_1:AO356_17540
         (837 letters)



>FitnessBrowser__Cup4G11:RR42_RS01795
          Length = 591

 Score =  305 bits (780), Expect = 6e-87
 Identities = 205/578 (35%), Positives = 304/578 (52%), Gaps = 41/578 (7%)

Query: 278 LHGVCAAPGLVTGPLVRLSGIQLPEDIGGHAIEEQR-----QRLSDALAQVRGEIHLTLE 332
           LHG+  + G+  G    L+   L  D+  + ++E R     +RL  A A VR E+ +TL+
Sbjct: 5   LHGIPVSRGVAIGRAHLLAPAAL--DVSHYLVDEDRLDAEVERLRSARAAVRAEL-VTLK 61

Query: 333 HARARQHRDEEAIF-SAHLALLEDPVLLDAADLFIEQGSA-APHAWSRSIDTQCQVLQQL 390
               R+  +E   F   H  +L+D  L    +  I      A  A +  ++   +   ++
Sbjct: 62  RDLPREAPEELGAFLDVHAMILDDEALAREPEALIRNRRYNAEWALTTRLEELMRQFDEI 121

Query: 391 GSTLLAERANDLRDLRQRVLRVLLGEAW-------------QFDVAAGAIVAAQELTPSD 437
               L ER  D+R + +R+L+VL G A                + A G IV A +++P+D
Sbjct: 122 EDEYLRERKADIRQVVERILKVLAGAAVLAPAPVPPSSLAPDGEPAPGVIVVAHDISPAD 181

Query: 438 LLQLSAQGVAGVCMVEGGATSHVAILARGKGLPCLVALGDELLAQEQGQAVVLDADGGRL 497
           +LQ       G     GG TSH AI+AR   +P  V +        Q   V++D D G +
Sbjct: 182 MLQFRHTVFHGFVTDLGGRTSHTAIVARSLDIPAAVGVQSASELIRQDDWVIIDGDAGLV 241

Query: 498 ELTPTVERLAQVRQAQTRRTALRAQQQSLAHTPARTVDGVEVEVAANVASSAEAGESLAN 557
            + P+   L + R  Q+ R   + + Q L HTPA T+DG+E+++ AN+  + +A  +L  
Sbjct: 242 IVDPSAIILEEYRHRQSERALEKKRLQRLRHTPAVTLDGLEIDLLANIEMAEDATAALTA 301

Query: 558 GADGVGLLRTEFLFVDRH-TAPDEEEQRQAYQAVLEAMGDKPVIIRTIDVGGDKQLDYLP 616
           GA GVGL R+EFLF++R    PDEEEQ +AY+  ++AM   PV IRTID+G DK LD   
Sbjct: 302 GAVGVGLFRSEFLFMNRRGELPDEEEQFEAYRGAVDAMHGLPVTIRTIDIGADKPLDGRD 361

Query: 617 --------LPSEANPVLGLRGIRLAQVRPDLLDQQLRALLQTRPLDRCRILLPMVT---E 665
                     +  NP LGLR IR +   P +   QLRALL+       R+L+PM+    E
Sbjct: 362 GRDGRGDDFETALNPALGLRAIRWSLSEPAMFLTQLRALLRASAFGPVRLLIPMLAHARE 421

Query: 666 VDELLHI----RKRLDALGSELGLSERPQLGVMVEVPAAALLAEQLAEHADFLSIGTNDL 721
           +D+ L +    +++LDA G         ++G M+E+PAA L+        DFLSIGTNDL
Sbjct: 422 IDQTLDLIAQAKRQLDARGQ--AFDPNIKVGAMIEIPAAVLMLPLFLRRMDFLSIGTNDL 479

Query: 722 SQYTLAMDRDHAGLAARVDALHPALLRLIAQTCAGAAKHGRWVGVCGALASDPLATPVLI 781
            QYTLA+DR    +A   D LHPA+L+LIA+T   A + G  V VCG +A DP  T +L+
Sbjct: 480 IQYTLAIDRADNAVAHLYDPLHPAVLQLIARTIREANRAGVPVAVCGEMAGDPAMTRLLL 539

Query: 782 GLGVRELSVSPPQIGEIKDRVRHLDAAQCARLSNELLN 819
           G+G+RE S+ P Q+  +K  V H D  +  RL  ++L+
Sbjct: 540 GMGLREFSMHPSQLLRVKQEVLHADCERLERLVEQVLS 577


Lambda     K      H
   0.319    0.134    0.386 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1023
Number of extensions: 43
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 837
Length of database: 591
Length adjustment: 39
Effective length of query: 798
Effective length of database: 552
Effective search space:   440496
Effective search space used:   440496
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources