Align Acetate/haloacid transporter, Dehp2, with a possible atypical topology (characterized)
to candidate RR42_RS03060 RR42_RS03060 MFS transporter
Query= TCDB::F8SVK1 (552 letters) >FitnessBrowser__Cup4G11:RR42_RS03060 Length = 566 Score = 774 bits (1998), Expect = 0.0 Identities = 386/561 (68%), Positives = 458/561 (81%), Gaps = 10/561 (1%) Query: 1 MATVSGQISHAPMTKEEKRVIFASSLGTVFEWYDFYLAGSLAAFISKSFFSGVNPTAAFI 60 +A G ++ MT EE++VIFASSLGTVFEWYDFYL GSLAA I+K FFSG++PTAAFI Sbjct: 7 VAIPGGNAQNSAMTAEERKVIFASSLGTVFEWYDFYLYGSLAAIIAKQFFSGLDPTAAFI 66 Query: 61 FTLLGFAAGFAVRPFGALVFGRLGDMVGRKYTFLITIVIMGLSTCVVGFLPGYAAIGMAS 120 F LL FAAGF VRPFGALVFGRLGDM+GRKYTFL+TI+IMG ST +VG LPG+A+IG A+ Sbjct: 67 FALLAFAAGFIVRPFGALVFGRLGDMIGRKYTFLVTILIMGASTFIVGLLPGFASIGWAA 126 Query: 121 PVIFIAMRLLQGLALGGEYGGAATYVAEHAPANRRGFYTAWIQTTATLGLFLSLLVILGV 180 P+I I +R+LQGLALGGEYGGAATYVAEHAP +RG YTAWIQTTATLGLFLSL+VIL Sbjct: 127 PIILIGLRMLQGLALGGEYGGAATYVAEHAPHGKRGAYTAWIQTTATLGLFLSLIVILVC 186 Query: 181 RTAMGEDAFGAWGWRIPFVASLVLLGISVWIRMQLHESPAFERIKAEGKTSKAPLSEAFG 240 R G + F WGWRIPF+ S++LLG+SV+IR+ + ESPAF+++KAEGKTSKAPL+EAFG Sbjct: 187 RNMTGAN-FEVWGWRIPFLVSILLLGVSVYIRLSMSESPAFQKMKAEGKTSKAPLTEAFG 245 Query: 241 QWKNLKIVILALIGVTAGQAVVWYTGQFYALFFLTQTLKVDGASANILIAIALLIGTPFF 300 QW+NLKIVILALIG+TAGQAVVWYTGQFY+LFFLTQ LKVD +AN+LIA AL++GTPFF Sbjct: 246 QWRNLKIVILALIGLTAGQAVVWYTGQFYSLFFLTQVLKVDATTANLLIAGALVVGTPFF 305 Query: 301 LFFGSLSDRIGRKPIILAGCLIAALTYFPLFKALTHYANPALEAATQKSPIVVIANPDEC 360 +FFGSLSD++GRK II+AGC +A LTYFPLFKALTHYANPALE A Q + IVV A+P +C Sbjct: 306 IFFGSLSDKVGRKWIIMAGCALAMLTYFPLFKALTHYANPALERAQQSAQIVVTADPKQC 365 Query: 361 SFQFNPVGTS-KFTSSCDIAKSALSKAGLNYDNVAAPAGTLAQIKVGDTTIDTYDGKAAD 419 SFQ +P+ F SSCDI K L++A +Y+ V APAGT+A +K+GD I +++ Sbjct: 366 SFQGSPIAREIDFRSSCDIVKRTLAQASASYEVVEAPAGTVASVKIGDKEIASFNASLVP 425 Query: 420 A----KDAGK----AFDKNLGTALKAASYPPKADPSQLNWPMTVVILTILVIYVTMVYGP 471 A DA K AF K +G ++ +A YP KADP+Q+N M +V+L ILVIYVTMVYGP Sbjct: 426 AGHSFDDASKKQIAAFKKQVGESMASAGYPTKADPAQMNTIMVLVVLVILVIYVTMVYGP 485 Query: 472 IAAMLVEMFPTRIRYTSMSLPYHIGNGWFGGFLPATAFAIVAAKGNIYSGLWYPIIIALA 531 IAAMLVEMFPTRIRYTSMSLPYHIGNGWFGG LP +FA+VA GNIY GLWYPIIIA Sbjct: 486 IAAMLVEMFPTRIRYTSMSLPYHIGNGWFGGLLPTISFALVAQNGNIYYGLWYPIIIAGV 545 Query: 532 TFVIGLLFVRETKDSNIYAQD 552 T V+G LF+RETKD +IYA D Sbjct: 546 TLVLGSLFIRETKDVDIYAND 566 Lambda K H 0.325 0.139 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 890 Number of extensions: 36 Number of successful extensions: 8 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 552 Length of database: 566 Length adjustment: 36 Effective length of query: 516 Effective length of database: 530 Effective search space: 273480 Effective search space used: 273480 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory