Alignments for a candidate for araG in Cupriavidus basilensis 4G11

GapMind for catabolism of small carbon sources

Alignments for a candidate for araG in Cupriavidus basilensis 4G11

Align L-arabinose ABC transporter, ATP-binding protein AraG; EC 3.6.3.17 (characterized)
to candidate RR42_RS30800 RR42_RS30800 sugar ABC transporter ATP-binding protein

Query= CharProtDB::CH_014279
         (504 letters)



>FitnessBrowser__Cup4G11:RR42_RS30800
          Length = 530

 Score =  236 bits (602), Expect = 1e-66
 Identities = 161/494 (32%), Positives = 251/494 (50%), Gaps = 25/494 (5%)

Query: 5   TPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGAGKSTLLKILSGNYAPTTGSV 64
           TP L   GI K F  + A  DIS +   G+V AL+GENGAGKSTL+ IL G+Y    G+V
Sbjct: 3   TPILRLAGITKRFGPLVANDDISLELQRGEVLALLGENGAGKSTLVSILFGHYVADAGTV 62

Query: 65  VINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAENIYLGQLPHKGGIVNRSLLNYEA 124
            ++GQ +      AAL AG+ +++Q   L   ++V +NI LG  P     ++      + 
Sbjct: 63  EVDGQPLPPGQPRAALTAGIGMVHQHFTLADNLSVLDNIMLGTQPLWQWRLDGHAARGKV 122

Query: 125 GLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALARNAKIIAFDEPTSSLSAREIDNLFR 184
               +  G+ + P   +  LS+G+ Q VEI KAL R A+++  DEPT+ L+  E + LF 
Sbjct: 123 LALAERFGLAVRPQARVGELSVGERQRVEIVKALYRGARVLILDEPTAVLTPHEAETLFS 182

Query: 185 VIRELRKEGRVILYVSHRMEEIFALSDAITVFKDGRYVKTFTDMQQVDHDALVQAMVGRD 244
            + +L  EG  ++++SH+++E+  +SD I V + G+ V      Q    + L + MVGR 
Sbjct: 183 TLAQLIAEGLSVIFISHKLDEVLRVSDRIAVLRGGKLVALCAAAQTTKAE-LAELMVGRV 241

Query: 245 I-------------GDIYGWQPRSYGEERLRLDAVKAPGVRTPISLAVRSGEIVGLFGLV 291
           +             G      P     E +   A     +   +SL VR+GEIVG+ G+ 
Sbjct: 242 VAMPERVARRSAEDGANGNAAPPVLALEHVGARAANGRALLREVSLQVRAGEIVGIAGVS 301

Query: 292 GAGRSELMKGMFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMMLCPEDRKAEGIIPVHSV 351
           G G++ L +   G  + + G++ +  +P+   KP   I AG+   PEDR A G++   +V
Sbjct: 302 GNGQAALAELASGMLEASEGRITLAGKPMS-AKPRAWIGAGVARVPEDRHAIGVVGDLAV 360

Query: 352 RDNI---NISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPGAEQLIMNLSGGNQQKA 408
            +N     +S  R    G  VI     +  A   +   +++T G +     +SGGN QK 
Sbjct: 361 WENAVSEQLSEPRFSRWG--VIRRAAAQRFARDLVARFDVRTAGIDVPARTMSGGNMQKL 418

Query: 409 ILGRWLS-----EEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGVAVLFASSDLPEVL 463
           ILGR LS        ++++  +PT G+D+GA   +   +   A +G AVL  S DL E+ 
Sbjct: 419 ILGRALSVRGEGSAPRLVVASQPTWGLDIGAVAYVRARLLDAAREGAAVLLISEDLDELH 478

Query: 464 GVADRIVVMREGEI 477
            +ADRI VM  G +
Sbjct: 479 ALADRIAVMHAGHL 492



 Score = 80.9 bits (198), Expect = 1e-19
 Identities = 66/233 (28%), Positives = 110/233 (47%), Gaps = 23/233 (9%)

Query: 275 ISLAVRSGEIVGLFGLVGAGRSELMKGMFGGTQITAGQVYIDQQPIDIRKPSHAIAAGM- 333
           ISL ++ GE++ L G  GAG+S L+  +FG     AG V +D QP+   +P  A+ AG+ 
Sbjct: 24  ISLELQRGEVLALLGENGAGKSTLVSILFGHYVADAGTVEVDGQPLPPGQPRAALTAGIG 83

Query: 334 MLCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPGA 393
           M+      A+ +    SV DNI +  +             W+     H  R   +     
Sbjct: 84  MVHQHFTLADNL----SVLDNIMLGTQPL-----------WQWRLDGHAARGKVLALAER 128

Query: 394 EQLIMN-------LSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALA 446
             L +        LS G +Q+  + + L    +V++LDEPT  +       +++ +  L 
Sbjct: 129 FGLAVRPQARVGELSVGERQRVEIVKALYRGARVLILDEPTAVLTPHEAETLFSTLAQLI 188

Query: 447 AQGVAVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKV 499
           A+G++V+F S  L EVL V+DRI V+R G++       Q  + +   L + +V
Sbjct: 189 AEGLSVIFISHKLDEVLRVSDRIAVLRGGKLVALCAAAQTTKAELAELMVGRV 241


Lambda     K      H
   0.319    0.136    0.391 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 663
Number of extensions: 37
Number of successful extensions: 9
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 504
Length of database: 530
Length adjustment: 35
Effective length of query: 469
Effective length of database: 495
Effective search space:   232155
Effective search space used:   232155
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources