Align ABC transporter for L-Arginine, putative ATPase component (characterized)
to candidate RR42_RS29660 RR42_RS29660 amino acid transporter
Query= reanno::BFirm:BPHYT_RS07685 (263 letters) >FitnessBrowser__Cup4G11:RR42_RS29660 Length = 264 Score = 449 bits (1156), Expect = e-131 Identities = 223/263 (84%), Positives = 244/263 (92%) Query: 1 MLHTTQTEACKLAVQDIHKRYGDNEVLKGVSLNANKGDVISIIGASGSGKSTFLRCINFL 60 ++ TTQ +CKLAVQDIHK +GDNEVLKGVSL A KGDVISIIGASGSGKSTFLRCIN+L Sbjct: 2 LMKTTQAMSCKLAVQDIHKSFGDNEVLKGVSLTAKKGDVISIIGASGSGKSTFLRCINYL 61 Query: 61 ERPNAGQIVVDGEMVKTKTDRAGNLEVADHKQLQRIRTKLAMVFQHFNLWAHMNVLENIV 120 ERP +GQI +DGE ++TK D+ G+L V + KQLQR+RTKL+MVFQHFNLWAHMNVLENI+ Sbjct: 62 ERPKSGQIFLDGEEIRTKKDKTGDLVVVEPKQLQRMRTKLSMVFQHFNLWAHMNVLENII 121 Query: 121 EAPIHVLGLKRKEAEDRAREYLEKVGLAPRLEKQYPSHLSGGQQQRVAIARALAMNPDVM 180 EAP HVLGL RKEAE+RAREYLEKVGL PR+EKQYPSHLSGGQQQRVAIARALAMNPDVM Sbjct: 122 EAPTHVLGLSRKEAEERAREYLEKVGLPPRVEKQYPSHLSGGQQQRVAIARALAMNPDVM 181 Query: 181 LFDEPTSALDPELVGEVLKVMQKLAEEGRTMIVVTHEMGFARNVSNHVMFLHQGRTEEEG 240 LFDEPTSALDPELVGEVLKVMQKLAEEGRTMIVVTHEMGFARNVSNHVMFLHQGRTEE+G Sbjct: 182 LFDEPTSALDPELVGEVLKVMQKLAEEGRTMIVVTHEMGFARNVSNHVMFLHQGRTEEQG 241 Query: 241 LPAEVLSAPRSERLKQFLSGSLK 263 P EVL++PRSERL+QFLSGSLK Sbjct: 242 PPEEVLNSPRSERLRQFLSGSLK 264 Lambda K H 0.318 0.133 0.374 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 325 Number of extensions: 6 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 263 Length of database: 264 Length adjustment: 25 Effective length of query: 238 Effective length of database: 239 Effective search space: 56882 Effective search space used: 56882 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 47 (22.7 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory