GapMind for catabolism of small carbon sources

 

Alignments for a candidate for artP in Cupriavidus basilensis 4G11

Align Arginine transport ATP-binding protein ArtM (characterized)
to candidate RR42_RS34305 RR42_RS34305 glutamine ABC transporter ATP-binding protein

Query= SwissProt::P54537
         (240 letters)



>FitnessBrowser__Cup4G11:RR42_RS34305
          Length = 252

 Score =  261 bits (668), Expect = 7e-75
 Identities = 132/240 (55%), Positives = 170/240 (70%)

Query: 1   MIKVEKLSKSFGKHEVLKNISTTIAEGEVVAVIGPSGSGKSTFLRCLNLLEKPNGGTITI 60
           MI+++ L K F  H VL+ +S  +  GEVV +IGPSGSGKST LRC+N LE   GG I  
Sbjct: 1   MIRIDNLQKYFDDHHVLRGVSLHVDAGEVVCLIGPSGSGKSTVLRCINGLESYEGGEIRA 60

Query: 61  KDTEITKPKTNTLKVRENIGMVFQHFHLFPHKTVLENIMYAPVNVKKESKQAAQEKAEDL 120
               + +   +  ++R  +GMVFQ F+LFPH++VLEN+M  PV VK ES+  A+ +A +L
Sbjct: 61  FGERVDRGGKDIHRLRSRMGMVFQRFNLFPHRSVLENVMEGPVYVKGESRDKARSEAMEL 120

Query: 121 LRKVGLFEKRNDYPNRLSGGQKQRVAIARALAMNPDIMLFDEPTSALDPEMVKEVLQVMK 180
           L KVGL  K   YP +LSGGQ+QRVAIARALAM P+ MLFDEPTSALDPE+V +VL+VM+
Sbjct: 121 LEKVGLAAKAQAYPPQLSGGQQQRVAIARALAMRPEAMLFDEPTSALDPELVGDVLEVMR 180

Query: 181 ELVETGMTMVIVTHEMGFAKEVADRVLFMDQGMIVEDGNPKEFFMSPKSKRAQDFLEKIL 240
            L   GMTM++VTHEMGFA+EVADRV F+  G IVE+G   +   +P   R QDFL ++L
Sbjct: 181 NLAREGMTMIVVTHEMGFAREVADRVCFLHSGTIVEEGAAAQVLGAPSHARTQDFLRRVL 240


Lambda     K      H
   0.317    0.134    0.368 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 186
Number of extensions: 3
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 240
Length of database: 252
Length adjustment: 24
Effective length of query: 216
Effective length of database: 228
Effective search space:    49248
Effective search space used:    49248
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 46 (22.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory