Align ATP-binding component of a broad range amino acid ABC transporter (characterized, see rationale)
to candidate RR42_RS00210 RR42_RS00210 ABC transporter ATP-binding protein
Query= uniprot:Q1MCU3 (247 letters) >FitnessBrowser__Cup4G11:RR42_RS00210 Length = 241 Score = 216 bits (550), Expect = 3e-61 Identities = 116/231 (50%), Positives = 154/231 (66%), Gaps = 2/231 (0%) Query: 12 LQVNGVETYYGNIRALAGVDVHVNKGEIVSLIGANGAGKSTLMMTICGSPQARTGSVVFE 71 L V G+ T Y + L V + V G+I ++GANGAGKSTL+ I G R G V+++ Sbjct: 10 LSVKGLTTGYDKVNVLHDVSIDVAPGKITCILGANGAGKSTLIRAILGLTPPRQGQVLWD 69 Query: 72 GRDITRMPTHEIARLRIAQSPEGRRIFPRMTVLENLQMGAGLD-NLKHFAEDVEKIFTLF 130 G+D+ TH I IA PEGR+IFPRMTV ENL +GA L+ + + + K++ +F Sbjct: 70 GKDLAGEKTHNIIATGIACIPEGRKIFPRMTVAENLALGAYLETDAARVRDRLAKVYDIF 129 Query: 131 PRLKERHAQRGGTLSGGEQQMLSIGRALMARPKLLLLDEPSLGLAPLIVKGIFEAIRKLN 190 PRLKER Q GT+SGGEQ M+SIGR LMA PKLL++DEPSLGL+PL VK F+ I+++N Sbjct: 130 PRLKERATQLAGTMSGGEQAMVSIGRGLMAEPKLLVIDEPSLGLSPLYVKENFKVIKQIN 189 Query: 191 EAEGLTVFLVEQNAFAALRLSHRAYVMVNGKVTMSGSGKELLANPEVRAAY 241 A G+TV LVEQNA L +SH YV+ G+V G+ + L +N EVR+AY Sbjct: 190 -ALGITVLLVEQNARQTLAISHYGYVLSQGRVVAQGTAEALASNDEVRSAY 239 Lambda K H 0.320 0.137 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 190 Number of extensions: 8 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 247 Length of database: 241 Length adjustment: 24 Effective length of query: 223 Effective length of database: 217 Effective search space: 48391 Effective search space used: 48391 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 46 (22.3 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory