Alignments for a candidate for gabT in Cupriavidus basilensis 4G11

GapMind for catabolism of small carbon sources

Alignments for a candidate for gabT in Cupriavidus basilensis 4G11

Align 4-aminobutyrate-2-oxoglutarate transaminase (EC 2.6.1.19) (characterized)
to candidate RR42_RS26240 RR42_RS26240 4-aminobutyrate aminotransferase

Query= BRENDA::Q0K2K2
         (423 letters)



>FitnessBrowser__Cup4G11:RR42_RS26240
          Length = 420

 Score =  600 bits (1548), Expect = e-176
 Identities = 293/421 (69%), Positives = 344/421 (81%), Gaps = 1/421 (0%)

Query: 3   MKNLELNQRRTLATPRGVGVMCDFYADRAENATLWDVEGRAYTDFAAGIAVLNTGHRHPR 62
           MKN +L +R+  ATPRGVGVMC+FYA+RAEN+ LWDVEG  Y DFAAGIAVLNTGHRHPR
Sbjct: 1   MKNADLVRRKDAATPRGVGVMCNFYAERAENSELWDVEGNRYIDFAAGIAVLNTGHRHPR 60

Query: 63  VMQAIAAQLERFTHTAYQIVPYQGYVTLAERINALVPIQGLNKTALFTTGAEAVENAIKI 122
           ++QA+ AQ+ERFTHTAYQIVPY  Y+ LAE+INA  P     KTA FTTGAEAVENAIKI
Sbjct: 61  LVQAMQAQMERFTHTAYQIVPYASYIELAEKINARAPGAFAKKTAFFTTGAEAVENAIKI 120

Query: 123 ARAHTGRPGVIAFSGAFHGRTLLGMALTGKVAPYKIGFGPFPSDIYHAPFPSALHGVSTE 182
           ARA TGRPGVIAFSG FHGRT++GMALTGKV PYK+GFGPFP D++HAP+P  LHGVS +
Sbjct: 121 ARAATGRPGVIAFSGGFHGRTMMGMALTGKVVPYKVGFGPFPGDVFHAPYPYGLHGVSVQ 180

Query: 183 RALQALEGLFKTDIDPARVAAIIVEPVQGEGGFQAAPADFMRGLRAVCDQHGIVLIADEV 242
            ++ AL  LFK D+DP RVAAII EPVQGEGGF  APA+F+R LRA+CD+HGI+L+ADEV
Sbjct: 181 DSINALHQLFKADVDPKRVAAIIFEPVQGEGGFNVAPAEFVRALRAICDEHGILLVADEV 240

Query: 243 QTGFGRTGKMFAMSHHDVEPDLITMAKSLAGGMPLSAVSGRAAIMDAPLPGGLGGTYAGN 302
           QTGFGRTGK+FAM H+DV PDL TMAKSLAGGMPLSAV GRA IMDAP PGGLGGTYAGN
Sbjct: 241 QTGFGRTGKLFAMEHYDVTPDLTTMAKSLAGGMPLSAVCGRAEIMDAPAPGGLGGTYAGN 300

Query: 303 PLAVAAAHAVIDVIEEEKLCERSASLGQQLREHLLAQRKHCPAMAEVRGLGSMVAAEFCD 362
           PLAVA+A AV+DV+E EKL ER A+LGQ+L++ L   +   P + EVRG+G+M+A EF  
Sbjct: 301 PLAVASALAVLDVLESEKLIERGAALGQRLQDKLDGLKSRVPEIGEVRGVGAMIAVEF-R 359

Query: 363 PATGQPSAEHAKRVQTRALEAGLVLLTCGTYGNVIRFLYPLTIPQAQFDAALAVLTQALA 422
            A G+P  E  ++VQ RALE GL+LL+CG YGNV+RFL+PLTIP A  D  L +L  AL 
Sbjct: 360 KADGRPDPEFTRQVQDRALERGLLLLSCGVYGNVVRFLFPLTIPDAVMDEGLGILEAALL 419

Query: 423 E 423
           +
Sbjct: 420 Q 420


Lambda     K      H
   0.321    0.136    0.400 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 631
Number of extensions: 16
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 423
Length of database: 420
Length adjustment: 32
Effective length of query: 391
Effective length of database: 388
Effective search space:   151708
Effective search space used:   151708
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 50 (23.9 bits)

Align candidate RR42_RS26240 RR42_RS26240 (4-aminobutyrate aminotransferase)
to HMM TIGR00700 (gabT: 4-aminobutyrate transaminase (EC 2.6.1.19))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.carbon/TIGR00700.hmm
# target sequence database:        /tmp/gapView.22357.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00700  [M=420]
Accession:   TIGR00700
Description: GABAtrnsam: 4-aminobutyrate transaminase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   7.9e-198  643.1   0.6   9.1e-198  642.9   0.6    1.0  1  lcl|FitnessBrowser__Cup4G11:RR42_RS26240  RR42_RS26240 4-aminobutyrate ami


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__Cup4G11:RR42_RS26240  RR42_RS26240 4-aminobutyrate aminotransferase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  642.9   0.6  9.1e-198  9.1e-198       2     418 ..       9     418 ..       8     420 .] 0.99

  Alignments for each domain:
  == domain 1  score: 642.9 bits;  conditional E-value: 9.1e-198
                                 TIGR00700   2 rraaavskGvgvtlrvlaakaegaelkdvdGnrlidlaagiavlnvGhshPkvveavkrqveelthtaf 70 
                                               r++aa+++Gvgv+++++a +ae++el dv+Gnr+id+aagiavln+Gh+hP++v+a++ q+e++thta+
  lcl|FitnessBrowser__Cup4G11:RR42_RS26240   9 RKDAATPRGVGVMCNFYAERAENSELWDVEGNRYIDFAAGIAVLNTGHRHPRLVQAMQAQMERFTHTAY 77 
                                               889****************************************************************** PP

                                 TIGR00700  71 qvvpyesyvelaeklnaiaPgsgekkavllnsGaeavenavkiarkytgrpgvvafsrgfhGrtnltma 139
                                               q+vpy sy+elaek+na aPg+  kk++++++Gaeavena+kiar+ tgrpgv+afs+gfhGrt + ma
  lcl|FitnessBrowser__Cup4G11:RR42_RS26240  78 QIVPYASYIELAEKINARAPGAFAKKTAFFTTGAEAVENAIKIARAATGRPGVIAFSGGFHGRTMMGMA 146
                                               ********************************************************************* PP

                                 TIGR00700 140 ltakvkPykiGfGPfapevyraPlpydyrdialdkqeslddelaaiealfvadveaeqvaavvlePvqG 208
                                               lt+kv Pyk+GfGPf+++v++aP+py +++ ++  q+s++    a+++lf+adv++++vaa+++ePvqG
  lcl|FitnessBrowser__Cup4G11:RR42_RS26240 147 LTGKVVPYKVGFGPFPGDVFHAPYPYGLHGVSV--QDSIN----ALHQLFKADVDPKRVAAIIFEPVQG 209
                                               ******************************999..88877....************************* PP

                                 TIGR00700 209 eGGfivpakelvaavaslckehgivliadevqtGfartGklfaieheddkPdlitvaksladGlPlsgv 277
                                               eGGf+v++ e+v a++ +c+ehgi+l+adevqtGf rtGklfa+eh+d++Pdl t+aksla+G+Pls+v
  lcl|FitnessBrowser__Cup4G11:RR42_RS26240 210 EGGFNVAPAEFVRALRAICDEHGILLVADEVQTGFGRTGKLFAMEHYDVTPDLTTMAKSLAGGMPLSAV 278
                                               ********************************************************************* PP

                                 TIGR00700 278 vGraeildapapGglGGtyaGnPlavaaalavldiieeeglieraeqigklvkdklielkeevpaigdv 346
                                                Graei+dapapGglGGtyaGnPlava+alavld++e+e lier   +g++++dkl  lk  vp ig+v
  lcl|FitnessBrowser__Cup4G11:RR42_RS26240 279 CGRAEIMDAPAPGGLGGTYAGNPLAVASALAVLDVLESEKLIERGAALGQRLQDKLDGLKSRVPEIGEV 347
                                               ********************************************************************* PP

                                 TIGR00700 347 rglGamiavelvdpdttePdaalaekiaaaalaaGlllltaGifGniirlltPltisdelldeglkile 415
                                               rg Gamiave+ ++d   Pd   +++++ +al++Gllll++G++Gn++r+l Plti+d+ +degl ile
  lcl|FitnessBrowser__Cup4G11:RR42_RS26240 348 RGVGAMIAVEFRKADG-RPDPEFTRQVQDRALERGLLLLSCGVYGNVVRFLFPLTIPDAVMDEGLGILE 415
                                               ************9986.6*************************************************** PP

                                 TIGR00700 416 aal 418
                                               aal
  lcl|FitnessBrowser__Cup4G11:RR42_RS26240 416 AAL 418
                                               *98 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (420 nodes)
Target sequences:                          1  (420 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.01
# Mc/sec: 9.13
//
[ok]

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources