Alignments for a candidate for rocE in Cupriavidus basilensis 4G11

GapMind for catabolism of small carbon sources

Alignments for a candidate for rocE in Cupriavidus basilensis 4G11

Align Amino-acid permease RocE (characterized)
to candidate RR42_RS11100 RR42_RS11100 gamma-aminobutyrate permease

Query= SwissProt::P39137
         (467 letters)



>FitnessBrowser__Cup4G11:RR42_RS11100
          Length = 509

 Score =  373 bits (958), Expect = e-108
 Identities = 194/466 (41%), Positives = 285/466 (61%), Gaps = 18/466 (3%)

Query: 9   NQLQRTMKSRHLFMISLGGVIGTGFFLGTGFTINQAGPLGAVLSYLVGGFIMFLTMLCLG 68
           + LQR +K+RHL MI++GG +GTG F+ +G +I+QAGP GA+L Y + G +++  M  LG
Sbjct: 16  DDLQRKLKARHLTMIAIGGAVGTGLFVASGASISQAGPGGALLMYCLIGLMVYCLMTSLG 75

Query: 69  ELAVAFPVSGSFQTYATKFISPAFGFAFGWLYWLGWAVTCAIEFLSAGQLMQRWFPHIDV 128
           ELAV  PV+GSF TY+  ++   FGFA GW YW   AVT A+E  +A  +MQ WFPH+  
Sbjct: 76  ELAVHMPVAGSFVTYSALYVEEGFGFALGWSYWFSLAVTIAVELAAAQLVMQYWFPHVSG 135

Query: 129 WIWCLVFAALMFILNAITTKAFAESEFWFSGIKILIILLFIILGGAAMFGLIDLKGGEQA 188
            +W   F  LMF LNA + + F E+E+WF+ IK+  IL+F+  G   +FG+  ++GG Q+
Sbjct: 136 VVWSAGFLLLMFGLNAFSVRGFGEAEYWFALIKVATILIFLAAGLMMIFGI--MQGGPQS 193

Query: 189 PFLTHFYEDGLFPNGIKAMLITMITVNFAFQGTELIGVAAGESEDPEKTIPRSIKQTVWR 248
            +      D  F  GI AM    +   F+FQGTE +GVAAGE+ DP +TIPR+I+QT WR
Sbjct: 194 GWHNFTLGDAPFVGGIPAMFGVAMIAGFSFQGTETVGVAAGEAADPARTIPRAIRQTFWR 253

Query: 249 TLVFFVLSIIVIAGMIPWKQAGVVE--------SPFVAVFEQIGIPYAADIMNFVILIAL 300
            L+F+VL+I++I  +IP+    ++         SPF  VF   G+ +AA +MN V+L AL
Sbjct: 254 ILLFYVLAILIIGVLIPYTDPSLLRNDVTDIGVSPFALVFRHAGLAFAAGLMNAVVLTAL 313

Query: 301 LSVANSGLYASTRILYAMANEGQAFKALGKTNQRGVPMYSLIVTMAVACLSLLTKFAQAE 360
           LS   S +YASTRILY +A  G+A +AL + +  GVP  +L  T AV  L  L+     +
Sbjct: 314 LSAGTSSMYASTRILYGLAVSGRAPRALARLSANGVPYVALFATTAVGALCFLSSLFGDK 373

Query: 361 TVYMVLLSLAGMSAQVGWITISLSQIMFRRKYIREGGKIEDLKFKTPLYPVLPLIGLTLN 420
            VY+ LL+ +GM+  + W+ I++S   FRR  + +G K  DL +++PLYP  PL  + L 
Sbjct: 374 AVYLWLLNTSGMTGFIAWLGIAISHYRFRRGLVHQGYKPSDLAYRSPLYPFGPLFAIVLC 433

Query: 421 TVVLISLAF----DPEQR----IALYCGVPFMIICYIIYHVVIKKR 458
            V+++   +    D   R    +  Y GVP  ++ ++ Y +V K R
Sbjct: 434 VVIVLGQNYQAFSDVRGRWLEIVGTYIGVPLFLVLWLGYRLVKKTR 479


Lambda     K      H
   0.329    0.142    0.438 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 678
Number of extensions: 29
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 467
Length of database: 509
Length adjustment: 34
Effective length of query: 433
Effective length of database: 475
Effective search space:   205675
Effective search space used:   205675
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources