GapMind for catabolism of small carbon sources

 

Aligments for a candidate for icd in Cupriavidus basilensis 4G11

Align isocitrate dehydrogenase (NAD+) (EC 1.1.1.41) (characterized)
to candidate RR42_RS29535 RR42_RS29535 isocitrate dehydrogenase

Query= BRENDA::Q945K7
         (374 letters)



>lcl|FitnessBrowser__Cup4G11:RR42_RS29535 RR42_RS29535 isocitrate
           dehydrogenase
          Length = 346

 Score =  269 bits (688), Expect = 7e-77
 Identities = 146/338 (43%), Positives = 208/338 (61%), Gaps = 11/338 (3%)

Query: 44  ITATLFPGDGIGPEIAESVKKVFTTAGVPIEWEEHYVGTEIDPRTQSFLTWESLESVRRN 103
           I  TL PGDGIGPE+ E+  KV    G P  W+    G          L   +L+S+RR 
Sbjct: 9   IPTTLIPGDGIGPEVVEATVKVLDALGAPFAWDIQQAGMAGIECGGDPLPDATLDSIRRT 68

Query: 104 KVGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSL-PGYKTRYDDVDLITIRENTE 162
            + LKGP+ TP+G G RS+N+ LR+  NLYANVRP  +L PG   R++D+DL+ +REN  
Sbjct: 69  GLALKGPLTTPVGGGFRSVNVRLREAFNLYANVRPARTLVPG---RFEDIDLVLVRENVG 125

Query: 163 GEYSGLEHQVVRG-----VVESLKIITRQASLRVAEYAFLYAKTHGRERVSAIHKANIMQ 217
           G Y   ++ +  G     V  S    TR A  R+A +AF YA  +GR++++ +HKANI++
Sbjct: 126 GFYVAHDYYIPVGDDPHAVAVSTGTNTRDACRRIARFAFEYAVRNGRKKITVVHKANILK 185

Query: 218 KTDGLFLKCCREVAEKYPE-ITYEEVVIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCA 276
              G+FL   REVA+ Y + +  ++ ++D C M LV NP  FD+L+  NL+GDI+SD  A
Sbjct: 186 ALTGIFLDAAREVAKDYADRVIMDDRIVDACAMQLVLNPWQFDMLLCTNLFGDILSDQIA 245

Query: 277 GLVGGLGLTPSCNIGEDGVALAEAVHGSAPDIAGKNLANPTALLLSGVMMLRHLKFNEQA 336
           GLVGGLG+ P  NIGE   A+ EAVHGSAPDIAGK +ANP +L+L+  +ML H+   + A
Sbjct: 246 GLVGGLGMAPGANIGEHA-AIFEAVHGSAPDIAGKGIANPISLMLAAGLMLEHVGRQDMA 304

Query: 337 EQIHSAIINTIAEGKYRTADLGGSSTTTEFTKAICDHL 374
            ++  AI  T+   + +T DL G+++T  F  A+   +
Sbjct: 305 LRLRQAIEQTLNVDQVKTGDLKGTASTLHFADAVAKRI 342


Lambda     K      H
   0.318    0.134    0.389 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 307
Number of extensions: 15
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 374
Length of database: 346
Length adjustment: 29
Effective length of query: 345
Effective length of database: 317
Effective search space:   109365
Effective search space used:   109365
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 49 (23.5 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory