GapMind for catabolism of small carbon sources

 

Alignments for a candidate for PS417_17605 in Cupriavidus basilensis 4G11

Align ATP-binding cassette domain-containing protein; SubName: Full=Amino acid transporter; SubName: Full=Histidine ABC transporter ATP-binding protein; SubName: Full=Histidine transport system ATP-binding protein (characterized, see rationale)
to candidate RR42_RS16445 RR42_RS16445 hypothetical protein

Query= uniprot:A0A1N7U8S3
         (276 letters)



>FitnessBrowser__Cup4G11:RR42_RS16445
          Length = 252

 Score =  237 bits (605), Expect = 2e-67
 Identities = 128/252 (50%), Positives = 175/252 (69%), Gaps = 3/252 (1%)

Query: 27  LQVEGIHKRYGEHEVLKGVSLNARQGDVISLIGASGSGKSTMLRCINFLEQPDAGVITLD 86
           + V  ++K +   + L GV+L  ++G+V+ LIG SGSGKST+LR IN+LE+P  G I++D
Sbjct: 2   IDVRDLYKHFDAVKALNGVNLEVQRGEVVCLIGPSGSGKSTLLRSINYLERPTRGDISID 61

Query: 87  GISI-EMRQGRAGTRAPHQDQLQNLRTRLAMVFQHFNLWSHMTVLENITMAPRRVLDVSA 145
           G ++  + Q     R     +L  +R+ + MVFQ F LW H++VLEN+T+    V  V  
Sbjct: 62  GQTVGHVNQAGKKARPMSGRELARVRSEVGMVFQMFYLWPHLSVLENVTLGMVEVRGVRL 121

Query: 146 AEAEKRARMYLDKVGLPSRVADQYPAFLSGGQQQRVAIARALAMEPEIILFDEPTSALDP 205
           A+A +R R  L KVGL  +  D YP  LSGGQ+QRVAIARALAM+P+IILFDEPTSALDP
Sbjct: 122 ADAAQRGRQLLAKVGLSGKY-DAYPEQLSGGQRQRVAIARALAMDPKIILFDEPTSALDP 180

Query: 206 ELVGEVLKVIQTLAEEGRTMLMVTHEMGFARQVSSQVLFLHQGRVEEHGD-ARILDQPNS 264
           E VGEVL+ ++T+A EG TM++ THEMGFAR+V+ +V+F+ +GRV E G    I D+P S
Sbjct: 181 ERVGEVLQTMETVAAEGMTMIVATHEMGFARRVADRVVFMDEGRVVEAGSPGDIFDRPQS 240

Query: 265 ERLQQFLSNRLK 276
            RL+QFL   L+
Sbjct: 241 ARLKQFLDKVLQ 252


Lambda     K      H
   0.319    0.133    0.372 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 232
Number of extensions: 12
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 276
Length of database: 252
Length adjustment: 25
Effective length of query: 251
Effective length of database: 227
Effective search space:    56977
Effective search space used:    56977
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 47 (22.7 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory